Method for extracting taurine and polysaccharides from abalone's viscera simultaneously

A technology of abalone viscera and taurine is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., and can solve the problems of cumbersome process, large pollution, large energy consumption, etc., so as to overcome the cumbersome process and reduce production. cost, and the effect of improving economic efficiency

Active Publication Date: 2016-01-27
JIMEI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

This kind of process is cumbersome and requires large-scale equipment, the cost is high, the ion exchange is used to remove impurities, the operation is complicated, and the regeneration of the ion exchange resin requires a large amount of acid and alkali, which pollutes the environment greatly.
The authorized patent (patent number: ZL201310283931.2) discloses a simple method for extracting natural taurine, which uses simple methods such as raw material pretreatment, ethanol removal of impurities, concentration, and crystallization to realize the preparation of taurine. The disadvantages are: ethanol impurity removal, crystallization, and recrystallization steps all need to add 3 to 4 times the volume of absolute ethanol, the consumption of ethanol is huge, the production cost is high, ethanol is flammable and explosive, and there are also great potential safety hazards
None of these patents or patent applications has developed and utilized taurine resources in abalone, resulting in a waste of resources, and the process is cumbersome and consumes a lot of energy.

Method used

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  • Method for extracting taurine and polysaccharides from abalone's viscera simultaneously

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Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: Extraction of natural taurine and abalone polysaccharide in abalone viscera

[0036] (1) Source of raw materials: 2kg of cleaned abalone viscera;

[0037] (2) Water extraction: Add 6L of water, mash the tissue, heat in a water bath at 80°C for 3 hours, and filter with gauze to obtain 7.50L of filtrate;

[0038] (3) Ultra-fol ultrafiltration membrane treatment: 7.50L of the collected filtrate is pumped into the Ultra-fol ultrafiltration membrane equipment for membrane separation treatment, and 700mL of water is added for top washing to obtain 7.60L of filtrate and membrane liquid 500mL;

[0039] (4) Enzymolysis: Add 0.5% by weight of neutral protease to the obtained 500mL membrane liquid, and enzymatically hydrolyze at 55°C for 2.5h;

[0040](5) Alcohol precipitation: collect the enzymatic solution, add 2 times the volume ratio of 95% ethanol solution, let it settle at room temperature for 12 hours, centrifuge (6000rpm, 20min) to collect the precipitate...

Embodiment 2

[0043] Embodiment 2: Extraction of taurine and abalone polysaccharide in abalone viscera

[0044] (1) Source of raw materials: 5kg of cleaned abalone viscera;

[0045] (2) Water extraction: add 20L of water, mash the tissue, heat in a water bath at 90°C for 2 hours, and filter with gauze to obtain a filtrate of 19.47L;

[0046] (3) Ultra-fol ultrafiltration membrane treatment: 19.47L of the collected filtrate was pumped into the Ultra-fol ultrafiltration membrane equipment for membrane separation treatment, and 1L of water was added for top washing to obtain 19L of filtrate and 1.25 L;

[0047] (4) Enzymatic hydrolysis: add 0.5% by weight of neutral protease to the obtained 1.25L of membrane inner solution, and enzymolyze at 55°C for 2.5h;

[0048] (5) Alcohol precipitation: collect the enzymatic solution, add 4 times the volume ratio of 95% ethanol solution, let it settle at room temperature for 24 hours, centrifuge (6000rpm, 20min) to collect the precipitate, and obtain ...

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Abstract

The invention provides a method for extracting taurine and polysaccharides from abalone's viscera simultaneously. The method comprises following steps: waste collection of viscera produced in an abalone processing process; water extraction; Ultra-fol ultrafiltration membrane treatment; enzymolysis; ethanol precipitation; concentration; crystallization; acquisition of high-purity taurine crystals after recrystallization. The method overcomes the technical defect that the taurine and the polysaccharides cannot be extracted simultaneously from the abalone's viscera with the conventional method and adopts the technical scheme including water extraction, Ultra-fol ultrafiltration membrane treatment, enzymolysis, ethanol precipitation, concentration and crystallization to extract the taurine and the polysaccharides from abalone's viscera simultaneously, so that the utilization rate of the abalone resources is increased effectively. The method solves the problems of complicated process, higher cost and serious environmental pollution during preparation of natural taurine with a traditional ion exchange method, overcomes the defect of large ethyl alcohol consumption during preparation of the natural taurine with a conventional simple preparation method and solves the problems of complicated process and large consumption of a conventional method for preparing the polysaccharides from abalone.

Description

Technical field [0001] The present invention involves the field of marine products, and it also involves a method of extracting taurine and polysaccharides from abalone internal organs. Background technique [0002] Abalone, with its unique flavor, texture and rich nutritional value, it sells at home and abroad.my country is the world's abalone breeding country. In 2010, the output of Chinese abalone breeding reached 56,500 tons, accounting for about 85%of the world's total output.In 2014, abalone production has exceeded 110,000 tons.At present, abalone is mainly based on fresh and canned products. With the increase of abalone production year by year and the development of abalone processing industry, the deep processing of abalone is particularly important.In the process of abalone processing, a large amount of soups and internal organs are often discarded, which not only causes waste of resources but also brings environmental pollution.On the other hand, with the development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C309/14C07C303/44C08B37/00
Inventor 曹敏杰崔璨章骞张凌晶蔡秋凤刘光明
Owner JIMEI UNIV
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