Method for extracting taurine and polysaccharides from abalone's viscera simultaneously
A technology of abalone viscera and taurine is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., and can solve the problems of cumbersome process, large pollution, large energy consumption, etc., so as to overcome the cumbersome process and reduce production. cost, and the effect of improving economic efficiency
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Embodiment 1
[0035] Embodiment 1: Extraction of natural taurine and abalone polysaccharide in abalone viscera
[0036] (1) Source of raw materials: 2kg of cleaned abalone viscera;
[0037] (2) Water extraction: Add 6L of water, mash the tissue, heat in a water bath at 80°C for 3 hours, and filter with gauze to obtain 7.50L of filtrate;
[0038] (3) Ultra-fol ultrafiltration membrane treatment: 7.50L of the collected filtrate is pumped into the Ultra-fol ultrafiltration membrane equipment for membrane separation treatment, and 700mL of water is added for top washing to obtain 7.60L of filtrate and membrane liquid 500mL;
[0039] (4) Enzymolysis: Add 0.5% by weight of neutral protease to the obtained 500mL membrane liquid, and enzymatically hydrolyze at 55°C for 2.5h;
[0040](5) Alcohol precipitation: collect the enzymatic solution, add 2 times the volume ratio of 95% ethanol solution, let it settle at room temperature for 12 hours, centrifuge (6000rpm, 20min) to collect the precipitate...
Embodiment 2
[0043] Embodiment 2: Extraction of taurine and abalone polysaccharide in abalone viscera
[0044] (1) Source of raw materials: 5kg of cleaned abalone viscera;
[0045] (2) Water extraction: add 20L of water, mash the tissue, heat in a water bath at 90°C for 2 hours, and filter with gauze to obtain a filtrate of 19.47L;
[0046] (3) Ultra-fol ultrafiltration membrane treatment: 19.47L of the collected filtrate was pumped into the Ultra-fol ultrafiltration membrane equipment for membrane separation treatment, and 1L of water was added for top washing to obtain 19L of filtrate and 1.25 L;
[0047] (4) Enzymatic hydrolysis: add 0.5% by weight of neutral protease to the obtained 1.25L of membrane inner solution, and enzymolyze at 55°C for 2.5h;
[0048] (5) Alcohol precipitation: collect the enzymatic solution, add 4 times the volume ratio of 95% ethanol solution, let it settle at room temperature for 24 hours, centrifuge (6000rpm, 20min) to collect the precipitate, and obtain ...
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