Method for controlling pH value and supplementing high-density fermentation acetobacter aceti in double-feeding mode

A high-density fermentation and Acetobacter technology, which is applied in the field of pH control and double-flow feeding and high-density fermentation of Acetobacter, can solve the problems of long cultivation period and achieve the effect of shortening the production period

Inactive Publication Date: 2018-05-08
SOUTH CHINA INST OF COLLABORATIVE INNOVATION
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese patent with the publication number CN103820368A discloses "Preparation method and application of acetic acid bacteria freeze-dried bacterial powder", which uses 5-25 wt% sodium hydroxide aqueous solution to maintain the pH of the fermentation medium, but the culture period is long and takes 20-30 hours

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for controlling pH value and supplementing high-density fermentation acetobacter aceti in double-feeding mode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of high-density fermentation method that controls pH and feeds double flow and adds acetic acid bacteria, comprises the steps:

[0019] (1) Strain activation: pick well-growing Acetobacter seeds from the preservation medium and inoculate them on the agar medium, and cultivate them at 30°C for 24 hours; the composition of the agar medium is: yeast extract 1%, glucose 1%, agar 2%, ethanol 2%, pH5.5~6.0, percentage is mass percentage, balance is water;

[0020] (2) Shake flask culture: inoculate the bacteria activated in step (1) into a shake flask, shake and cultivate for 18 hours, and obtain a seed liquid; shake flask medium formula: yeast extract 1%, glucose 1%, KH 2 PO 4 0.25%, ethanol 2%, pH 5.5-6.0, the percentages are mass percentages, and the balance is water; the liquid volume in a 250mL shaker flask is 50mL; culture conditions: shaker flask speed 150rpm, temperature 30°C;

[0021] (3) Seed tank expansion: the liquid cultivated in the shake flask is inse...

Embodiment 2

[0024] A kind of high-density fermentation method that controls pH and feeds double flow and adds acetic acid bacteria, comprises the steps:

[0025] (1) strain activation and shaking flask culture are the same as in Example 1;

[0026] (2) Seed tank expansion: the liquid cultivated in the shake flask is inserted into a 10L seed tank with an inoculation amount of 10% as the seed liquid; the liquid volume of the 10L seed tank is 60%; the composition of the medium: yeast extract 4%, glucose 5 %, KH 2 PO 4 0.75%, ethanol 2%, pH 5.5-6.0; culture conditions: stirring speed 300rpm, ventilation volume 1vvm, temperature 30°C, adding 5M sodium hydroxide, controlling the pH of the fermentation broth to 5.5-6.0, culture time 10h;

[0027] (3) Fermentation tank fermentation: Transplant by pressure difference, the seed liquid in the seed tank is inserted in the fermentation tank by 10% inoculum amount and fermented; 100L fermentation tank liquid volume 70%; Culture medium composition: ye...

Embodiment 3

[0029] A kind of high-density fermentation method that controls pH and feeds double flow and adds acetic acid bacteria, comprises the steps:

[0030] (1) Strain activation and shake flask cultivation are the same as in Example 1, and seed tank expansion is the same as in Example 2

[0031] (2) Fermentation tank fermentation: Transplant by pressure difference, the seed liquid in the seed tank is inserted in the fermentation tank by 10% inoculum amount and fermented; 100L fermentation tank liquid volume 70%; Culture medium composition: yeast extract 4 %, Glucose 5%, KH 2 PO 4 0.75%, ethanol 2%, pH 5.5-6.0; culture conditions: stirring speed 300rpm, air flow 1vvm, temperature 30°C, add 5M sodium hydroxide, control the pH of the fermentation broth to 5.5-6.0, and feed yeast at the same time The extract and corn steep liquor were cultured for 12 hours, and the yeast extract and corn steep liquor were added in an amount of 5 g / L, and the addition was completed within 5 hours afte...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of food biotechnology, and discloses a method for controlling a pH value and supplementing high-density fermentation acetobacter aceti in a double-feeding mode. Themethod comprises the following steps: (1) activating thallus; (2) performing shake-flask culture; (3) performing seed tank propagation; (4) performing fermentation tank fermentation: inoculating a seed solution obtained in step (3) on a culture medium in a fermentation tank to culture according to an inoculation amount of 1%-20%, wherein culture conditions are as follows: stirring rotation speed of 100-300 rpm, throughput of 0.1-1.0 vvm, a temperature of 28-30 DEG C; and feeding 5-10 M of sodium hydroxide, controlling the pH value of fermentation liquor to 5.5-6.0, and feeding yeast extract and corn pulp to culture for 8-12 hours. A way of controlling the pH value and adopting yeast extract and corn pulp to supplement in a double-feeding mode is adopted, so that the production period is greatly shortened, and therefore, more thallus can be enriched more quickly, and high-density fermentation of 1.56-3.34*10<-9> cfu/mL is realized.

Description

technical field [0001] The invention relates to a method for controlling pH and feeding double flow and adding high-density fermented acetic acid bacteria, which belongs to the field of food biotechnology. Background technique [0002] Acetobacter, Gram-negative, does not produce spores, can be used to make vinegar, aerobic respiration, and can oxidize ethanol (alcohol) into acetic acid under aerobic, neutral and acidic conditions. The content of alcohol and base acid in the fermentation medium had a significant effect on the growth and acid production of bacteria in acetic acid fermentation. Although Acetobacter has a certain tolerance to acetic acid, as the fermentation time prolongs, the acid production gradually increases, and Acetobacter may be killed by the acid produced by itself. [0003] Compared with the rapid development of the dairy industry, the research on Acetobacter inoculum in my country is relatively backward. The traditional fermentation process often ado...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/02
CPCC12N1/20
Inventor 杨继国刘艺婷任杰刘玮
Owner SOUTH CHINA INST OF COLLABORATIVE INNOVATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap