Method for preparing glutathione by fed-batch fermentation of Candida utilis

Abstract

The invention belongs to the field of microbial fermentation, and more particularly relates to a method for preparing glutathione by fed-batch fermentation of Candida utilis. The method comprises: taking the Candida utilis SZU 07-01 as original strain, carrying out slant cultivation and seed cultivation on the original strain, and fermenting for at least 42 hour; and fermentation more particularly comprises the steps of: after expansion of cultivation, inoculating seeds into a fermentation medium according to 5-10% of inoculation quantity, and controlling the fermentation temperature within the range of 28-32 DEG C, the stirring rotation speed within the range of 250-300rpm and the ventilation capacity within the range of 0.5-1.5vvm; furthermore, when the fermentation is carried out for 10-12h, feeding the fermentation medium in batch; and manually adjusting the rotation speed and the ventilation capacity, and controlling the concentration of dissolved oxygen to be more than or equal to 35%. By adopting multinomial fed-batch fermentation way, on the basis of meeting the demand for nutrient substance of yeast cells, the method further improves the cell density and GSH yield, obviously reduces the fermentation time and increases the production efficiency.

Description

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for preparing glutathione by fed-batch fermentation of Candida utilis. Background technique

[0002] Since the earliest patent on the production of GSH by yeast was published in 1938, foreign scholars have carried out a lot of research on the production of GSH. Generally speaking, the production methods of glutathione mainly include solvent extraction, chemical synthesis, and enzymatic conversion. and fermentation. The extraction method is mainly to separate and extract GSH from animal and plant tissues containing GSH by means of extraction and precipitation. Since the raw materials are not easy to obtain and the content of GSH is extremely low, the practical application value of this method is not great. GSH is produced by chemical synthesis, that is, L-glutamic acid, L-cysteine ​​and glycine are condensed into GSH. This method was applied to the prod...

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