153 results about "Candida pelliculosa" patented technology

The invention relates to a biotechnology dealing agricultural discard stem with an highly effective stem decomposing composite bacterial, which dissolves the problem of single species of applying microbiological bacterial, weak breaking up, low total degradability rate of cellulose, long developmental cycle. The composite bacterial comprises the following parts: yellow archespore wool bacterium leather bacterial, mushroom bacterial, haci trichoderma, green trichoderma, coninic trichoderma, aspergillus niger, bacillus subtilis, feed bacillus, bacillus megaterium, candida, distillery yeast, food-yeast, brown sphere azotobacter, thermoactinomyces, and so on. The composite bacterial can overall degrade lignin, cellulose, hemicellulose and other organic material in the stem, has a more than 80% degrading rate, a 10-15 days short cycle, keeps a long time for providing a solid bacteria.

The invention relates to candida Antarctica lipase B gene and applications thereof in yeast display. Coded protein of improved candida Antarctica B and wild type candida Antarctica lipase protein have the same function on the amino acid level; the heat resistance capacity of the enzyme is 50-80 DEG C, and the half-lift is 3-24 hours; a nucleotide sequence is hybridized with SEQ.ID.NO2 from 1st to 978th of nucleotide under the moderate precise condition; and a preservation number of colon bacillus DH5Alpha / Puc57-CALB (Escherichia coliDH5Alpha / pUC57-CALB) which carries the plasmids is CCTCC M 209081. The candida Antarctica lipase B gene is transferred into pichia pastoris host bacteria so as to realize the high-efficient display expression of the candida Antarctica lipase B in pichia pastoris; and the provided pichia pastoris bacteria can effectively display candida Antarctica lipase B, can be widely applied to the synthesis of ethyl caproate, has different melting points and does not contain triglyceride of various fatty acids, a plurality of structured lipids, and the like.

The invention discloses a microbial deodorizing inoculant which is used in refuse sites, public toilets and livestock / poultry cultivation farms. The microbial deodorizing inoculant contains Candida utilis of which the collection number is ACCC 20060, Lactobacillus plantarum of which the collection number is ACCC 11016, lactic streptococci of which the collection number is ACCC 10653, cerevisiae fermentum of which the collection number is ACCC 21135, Streptococcus thermophilus of which the collection number is ACCC 10651, Bacillus licheniformis of which the collection number is ACCC 01870, and Bacillus natto of which the collection number is ACCC 19833. The microbial deodorizing inoculant absorbs and degrades generated malodorous gas hydrogen sulfide and ammonia gas, eliminates the malodor of the garbage and garbage leachate, inhibits the putrefaction bacteria from growth, basically prevents the malodorous gas from generation, and forms a disease-spread-free refuse piling environment capable of eliminating the malodor.

Methods and compositions useful in the detection and identification of species of Candida are disclosed. These species include Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis, each of which is a causative agent for vaginal candidiasis. The compositions of the invention are combinations of oligonucleotides. These oligonucleotides include pairs of forward and reverse primers for polymerase chain reactions, wherein each primer pair is capable of priming the synthesis of an amplicon specific to one of Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis, but preferably is not capable of priming the synthesis of an amplicon specific to any of the other three species. In preferred embodiments, the forward primers of the primer pairs have identical sequences, while each reverse primer of the primer pairs has a unique sequence relative to all of the other reverse primers; or the reverse primers of the primer pairs have identical sequences, while each forward primer of the primer pairs has a unique sequence relative to all of the other forward primers. These unique primer sequences account for the species specificity of the resultant amplicons. The oligonucleotides also include probes capable of detecting these amplicons, and sequencing primers for determining, in primer extension reactions, the nucleotide sequences contained within the amplicons. In preferred methods of the invention, a biological sample is tested for the presence of at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis by isolating nucleic acid from the sample, attempting a polymerase chain reaction in a mixture containing this nucleic acid and a plurality of these primer pairs, ascertaining whether any amplicon is produced in the mixture using an oligonucleotide probe, and determining the sequence of any resultant amplicon using the sequencing primers. The detection of an amplicon indicates that the sample contains at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, or Candida tropicalis, and the nucleotide sequence data is used to determine which of these four Candida species is present.

The invention relates to the biological fermentation technology, in particular to a compound microbial starter, and a method which uses the compound microbial starter to produce high-energy protein biological feed. The compound microbial starter selects cellulomonas sp dinitrogen, cellulomonas flavigena, condensate bacillus, lactobacillus acidophilus, bacillus subtilis, lactobacillus starch, compound enzyme bacteria, propionic acid bacteria, candida yeast, saccharomyces diastaticus, saccharomyces cerevisiae, as well as aroma-producing yeast as the bacteria sources, and makes use of the modern biotechnology to prepare various microbial agents; the microbial agents are then treated with anaerobic fermentation with the discarded sweet corn cobs, corn stalks, pea skins, abandoned vegetables and other discarded crops, so as to obtain the high-energy protein biological feed required in livestock and poultry breeding industry. The invention solves the problem of low utilization rate of crop resources; and the high-energy protein biological feed can improve the palatability of feed, increase the enzyme content of the feed, provide an effective way for developing the animal husbandry and solving the food conflicts between people and livestock, as well as reduce the pollution to the environment caused by the traditional crop waste burning.

The invention discloses a fluorescence quantitative PCR primer, a probe and a kit for detecting ordinary pathogenic fungi. A specific primer and a TaqMan probe are independently designed, and a fluorescence PCR detection method which can be used for simultaneously detecting 15 clinically ordinary pathogenic fungi, including 8 candida mycoderma bacteria (candida albicans, candida glabrata, candida parapsilosis, candida kefyr, candida sake, candida kruse, candida guilliermind and candida tropicalis), four aspergilli (aspergillus niger, aspergillus flavus, aspergillus terreus and aspergillus fumigates), cryptococcus, rhizopus oryzae and mucor circinelloides, is established. The method is relatively high in sensitivity and specificity, and has significant meanings for early diagnosis and treatment on invasive infections with fungi.

The invention relates to a microorganism bacterium agent for treating industrial wastewater pollution. The microorganism bacterium agent is prepared from pseudomonas putida, candida utilis, bacillus amyloliquefaciens, clostridium papyrosolvens, nitrosomonas europaea, paracoccus denitrificans and auxiliary materials. The microorganism bacterium agent has the advantages that various bacteria in mutualistic symbiosis are compatible reasonably without antagonism and are effective in degradation of phenyl amines degradation-resistant compounds when being thrown into a wastewater treatment system, thereby being suitable for industrial wastewater treatment and being capable of increasing treatment water quantity, improving treatment water quality, reducing operational cost and promoting up-to-standard discharge.

The invention discloses a candida lipolytica strain and a method for preparing long chain dicarboxylic acid by using the candida lipolytica strain, belonging to the field of microbial fermentation. The candida lipolytica strain is candida lipolytica CX-998 CCTCC NO:M2011094. The method for preparing the long chain dicarboxylic acid comprises the following steps of: in a culture medium in which C10-C18 n-alkane is used as a substrate, using the candida lipolytica strain to perform fermentation; and separating, extracting and recycling the fermentation product to obtain the long chain dicarboxylic acid which is the same as an n-alkane substrate chain. According to the method, all sorts of single and mixed binary acids of C10-C18 can be produced, wherein in a 10-liter fermentation tank, when DC11 is produced from nC11 through fermentation transformation, the DC11 output in 144 hours reaches 165g / L, and the after-treatment yield is 88%; and when the purity of the nC11 is more than 99%, the purity of the DC11 reaches 99%.

The invention relates to new mutants of formate dehydrogenase from Candida boidinii, new gene sequences encoding these and use of the new formate dehydrogenases.The wild type FDH used hitherto in the industrial process for preparing amino acids becomes inactive after a certain time. New mutants of this wild type have been produced by targeted mutagenesis of a recombinant FDH from E. coli. The new mutants are preferably used in an enzymatic process for preparing chiral compounds.

The invention relates to a barton fermentation bed complex microbial inoculant and a preparation method thereof. The invention is characterized in that the complex microbial inoculant comprises the following six beneficial microorganisms in percentage by weight: 15-30 percent of Trichoderma koningii, 10-25 percent of geotrichum candidum, 10-25 percent of Candida utilis, 10-30 percent of lactobacillus plantarum, 10-25 percent of bacillus subtilis and 5-15 percent of bacillus subtilis natto. The complex microbial inoculant is prepared by a fermentation process, and the total number of fermentation viable bacteria in finished products is 2-5*10<9>cfu / ml. The microbial strains adopted by the complex microbial inoculant are safe and reliable, the finished viable bacteria content is high and the bioactivity is high; 1L / m<3> complex microbial inoculant is used on a barton fermentation bed, thus obviously reducing the odor in a barton, decreasing the breeding of mosquitoes and flies, having stronger degradation capability on chicken excrement, achieving the ecologically breeding purposes of no pollution and zero emission, enhancing the digestive absorption function of chicken, improving the chicken immunity, enhancing the broiler quality, increasing the quality of eggs, and ensuring the color of yolks to be brightly red.

The invention relates to the field of complex microorganisms, in particular to a feed additive with complex microorganisms for piglets. The feed additive consists of candida utilis, enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis, and then all the raw materials are cultured in culture medium containing 5-10 percent of epimedium herb for 60-72 hours at the temperature of 35-37 DEG C to obtain culture, namely the feed additive. According to weight part, the candida utilis : enterococcus faecalis : lactobacillus acidophilus : bacillus subtilis is equal to 1-3:1-3:1-3:1-3. The feed additive has multiple nutrient component, oregano extract has the functions of sterilization, bacteriostasis and oxidation resistance, can well inhibit the diarrhea of the piglets, enhance the immunity of the piglets, reduce the death rate and lower the cost.

Belonging to the field of microbial environmental protection, the invention provides a microbial agent for treatment of sewage and sludge. The microbial agent mainly includes Rhodospirillum rubrum, rhodopseudomonas palustris, bacillus subtilis, Streptomyces microflavus, streptococcus thermophilus, candida utilis, Streptomyces viridis, pseudomonas aeruginosa, virgibacillus halodenitrificans, and Paracoccus denitrificans 10 bacteria. Each bacterium is subjected to conventional slant culture, shaking culture and seed tank cultivation respectively, the bacteria in the seed tank are mixed and then put into sewage directly, symbiosis of flora is utilized to reach the purpose of treating sewage and reducing sludge generation. The microbial agent provided by the invention can be applied to large-scale production and direct adding, is low in cost, and can adapt to changeful sewage components. And the flora has good impact resistance to sewage.

The invention discloses a compound microbial inoculum for deodorization, which comprises the following active ingredients: Trichoderma viride, Candida utilis, Saccharomyces cerevisiae and Lactobacillus plantarum. As a green technology, the microbial deodorization technology has the advantages of low environmental impact and ecological sustainability, and can not produce secondary pollution. The microbial inoculum disclosed by the invention has the advantages of favorable deodorization performance, obvious deodorization effect and high practical value.

The invention provides a nucleotide sequence suitable for secretory expression of recombinant candida utilis uricase (uricase, EC1.7.3.3) by virtue of escherichia coli, a carrier containing the nucleotide sequence, an engineering cell containing the carrier, and a method for purifying the recombinant candida utilis uricase from the engineering cell. On the basis of a natural sequence of a uricase gene, a codon of the uricase gene is optimized, the transformed uricase gene is cloned to a prokaryotic expression vector pET42a(+); an escherichia coli Rossetta strain is transformed; the expression level can reach 50% of soluble protein of the escherichia coli by IPTG induction; the expressed protein is soluble protein; and the biological activity of the expressed protein can be up to that of 37.89U / mg recombinant protein. The recombinant candida utilis uricase product can be efficiently and simply obtained at low cost. The invention also comprises an application of the recombinant candida utilis uricase prepared by the method in preparation of medicines, foods, health care products and cosmetics for treating hyperuricemia-related diseases or pathological symptoms of a human body.

The present invention provides compositions useful as antimicrobial agents which include mammalian hemoglobin, the alpha and beta chains of hemoglobin free of heme, fragments of the alpha and beta chains that result from cyanogen bromide cleavage of the alpha and beta chains, and synthetic peptides derived therefrom. The compositions exert antimicrobial activity against both bacteria and fungi that is comparable to known antimicrobial peptides from human neutrophils, cathepsin G and azurocidin. Sensitive organisms include Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa, Gram-positive bacteria such as Staphylococcus aureus and Streptococcus faecalis, and the fungus Candida albicans. Methods for preparing the compositions also are provided.

The invention relates to a method for biotransformation of the main component notoginsenoside in the traditional Chinese medicine pseudo-ginseng by using saccharomycete; specifically speaking, the invention relates to a method for biotransformation of notoginsenoside by using Candida, especially by using Candida lambica. After transformation by the saccharomycete, the content of panax notoginseng saponins, especially the content of rare ginsenosides like ginsenoside Rg3 and Rh2, is substantially increased. The notoginsenoside transformed by Candida lambica can be used as a raw material for production of medicines, health products or cosmetics.

The invention discloses an ecological restoration microbial inoculum capable of inhibiting mildews and degrading mycotoxin. The ecological restoration microbial inoculum consists of bacillus subtilis, rhodopseudomonas palustris, enterococcus faecalis, wautersiellfalsenii and candida utilis which are mixed together. A preparation method of the ecological restoration microbial inoculum comprises the following steps: inoculating strains to corresponding sterilized culture medium for fermentation; performing three-stage amplification culture; and finally, adsorbing and solidifying on straw charcoal to prepare a solid microbial inoculum. The ecological restoration microbial inoculum disclosed by the invention has the advantages of being high in density and activity and excellent in characteristic. The bacillus subtilis, the rhodopseudomonas palustris, the enterococcus faecalis and the newly separated wautersiellfalsenii have an effect of inhibiting mildews and degrading mycotoxin, and particularly by cooperation with the candida utilis, the effect of degrading mycotoxin is obviously increased. The ecological restoration microbial inoculum can be used as a feed additive to solve the problem of mildew and mycotoxin pollution in the feed industry, and can also be used as a soil ecological restoration agent to solve the problem of the spreading of a continuous cropping disease in plantation of ginseng and notoginseng.

The invention provides recombinant saccharomyces cerevisiae for producing ethanol by xylose fermentation and a construction method thereof. In the invention, candida boidinii is led to the saccharomyces cerevisiae to metabolize a DNA fragment of a coding gene of related enzymes of the xylose, so that the saccharomyces cerevisiae can secrete and express xylose isomerase the enzymatic reaction conditions of which are close to growth conditions of the saccharomyces cerevisiae, so the recombinant saccharomyces cerevisiae can effectively utilize the xylose to produce the ethanol, thus solving the technical problem that the saccharomyces cerevisiae can not utilize the xylose to produce the ethanol. The invention lays a foundation for industrial application of the saccharomyces cerevisiae for producing the ethanol by using xylose fermentation, thus having great economic benefit and social benefit.

The invention relates to a composite fungicide for treating sludge and a sludge treatment method. The composite fungicide is prepared from the following components in parts by weight: 3 to 8 parts of purple sulfur bacteria, 3 to 8 parts of green sulfur bacteria, 3 to 8 parts of rhodopseudomonas, 3 to 8 parts of rhodopseudomonas capsulata, 3 to 8 parts of rhodococcus rhodochrous, 3 to 8 parts of white-rot fungi, 3 to 8 parts of phosphorus-accumulating bacteria, 3 to 8 parts of anaerobic ammonium oxidation bacteria, 3 to 8 parts of lactobacillus plantarum, 3 to 8 parts of streptomycete, 3 to 8 parts of candida utilis, as well as saccharomyces cerevisiae, lactobacillus lactis, lactobacillus acidophilus, aspergillus niger, aspergillus oryzae, bacillus subtilis and bacillus licheniformis.

The invention discloses a method for preparing selenium-enriched yeasts by using Candida utilis, which relates to a novel method for preparing selenium-enriched yeasts. The method for preparing the selenium-enriched yeasts comprises the following steps: a, reviving Candida utilis with a collection number of CICC 1422 and preparing seeds; b, inoculating the seeds obtained by the step a onto a fermentation medium to ferment the seeds for 24 to 36 hours; c, adding selenium into fermentation liquor till the concentration of the selenium is 5 to 20mg / L, continuing fermentation for 24 to 48 hours, and collecting bacterium solution; and d, centrifuging the bacterium solution obtained by the step c, collecting thalli, drying and obtaining the selenium-enriched yeasts. In the method, the production cost, the biomass of the selenium-enriched yeasts, the selenium content in the selenium-enriched yeast can be taken into better consideration at the same time, selenium-enriched yeasts with high biomass and high selenium content can be obtained under a condition of small inorganic selenium consumption, and the method is suitable for industrial production.

The invention discloses an expression carrier of an industrial microorganism that is candida utili and simultaneously provides a construction method of the candida utili expression carrier. The invention uses a basic skeleton of a pBR322 carrier and a template of candida utili genomic DNA, obtains a cycloheximide resistance gene with the site-directed mutagenesis method, obtains a rDNA segment and a GAP promoter-terminator segment of the candida utili genomic DNA by adopting the PCR method and the template of the candida utili genomic DNA, and constructs the integrated candida utili expression carrier that is mediated by 18S rDNA and has cycloheximide resistance. The integrated candida utili expression carrier can be used for preparing a foreign candida utili expression protein and can be widely applied into the fields of feedstuff addition agents, food industry, and pharmacy, and the like.

The invention relates to a feed additive, in particular to an oligopolymerization chitosan-astragalus polysaccharide ecological feed additive which comprises Candida utilis, enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis. The feed additive is prepared by culturing the follwing compositions in parts by weight: 1-3 parts of Candida utilis, 1-3 parts of enterococcus faecalis, 1-3 parts of lactobacillus acidophilus and 1-3 parts of bacillus subtilis in a culture medium for 60-72h under the temperature of 28-37 DEG C, wherein the culture medium comprises 1g of agar, 1g of beef extract, 2.5g of yeast extract, 35g of sugar, 1.5g of defoamer, 1g of peptone, 20-50g of oligopolymerization chitosan and 20-50g of astragalus polysaccharide. distilled water is added till the total volume is 1000 ml, and the pH is 6.0, and the mixture is treated by high pressure sterilization for 20m under the temperature of 121 DEG C for later use. The additive can fulfill multiple synergistic function to guarantee the effect and has the advantages of remarkable effect, convenient use and the like.

The invention provides a microecological preparation for breeding laying hens. The microecological preparation includes the following components: lactobacillus acidophilus, rumen bifidobacterial, candida utilis, xylanase, glucose oxidase, bacillus amyloliquefaciens, bacilluslicheniformis, bacillus subtilis, lipase, acidic cellulase, and acid protease. A spore content of bacillus amyloliquefaciensresting body microorganism dry powder is greater than or equal to 10<11> / g; the spore content of bacilluslicheniformis resting body microorganism dry powder is greater than or equal to 10<11> / g; and the spore content of bacillus subtilis resting body microorganism dry powder is greater than or equal to 10<11> / g. The microecological preparation has the characteristics of significantly improving thequality of eggshells of eggs, reducing the yield of the eggs with broken and soft shells, and improving egg production of Jinghong laying hens. Formation of feces of laying hens is significantly improved, and an ammoniacal odour of a hen house is significantly reduced.

The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g / L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g / L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity.

The invention discloses a method for producing glutathione through microbial fermentation, which is a method that protolysate and vitamin B complex are added in fermentation medium, and inorganic sulfur source is modified into organic sulfur source to greatly improve the yield of glutathione. The invention adopts Candida utilis as glutathione to produce bacterial strain, wherein the yield of glutathione in the fermentation medium containing protolysate, vitamin B complex and organic sulfur reaches 7 to 10 g / L, and the reduced form is larger than 98 percent. The invention has the advantages that the method greatly improves the yield of glutathione under the same other culture conditions, greatly reduces the production cost, and can change the situation of dependence of imported glutathione.

Disclosed herein are Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, a method of producing a high yield of xylitol using the same, and xylitol produced by the method. More specifically, disclosed are a method for producing a high yield of xylitol, in which a high concentration of xylose contained in a biomass hydrolyzate is converted to xylitol using xylitol dehydrogenase-inactivated mutant of Candida tropicalis, without controlling dissolved oxygen to a low level, as well as xylitol produced according to the method. Also disclosed are a xylitol production method, in which the production of byproduct arabitol, which is produced when using a biomass as a substrate and adversely affects the yield of xylitol, is significantly reduced through the use of Candida tropicalis mutant ara-89 (KCTC 11136bp) having an inhibited activity of arabinose reductase converting arabinose to arabitol, thus increasing xylitol productivity, as well as xylitol produced by the method.

This invention provides a method for preparing Candida utillis autolysate. The method comprises: placing the co-cultured product (endogenous enzyme activity is 60-100 U / g dry fungi) of Candida utillis and Aspergillus oryzae into an insulating container with a stirrer, adjusting the concentration of the solid to 10-30 wt.% of the yeast solution, adding 0.2-2.5 wt.% of NaCl into the yeast solution at a pH value of 4.5-6.8 and a temperature of 45-55 deg.C, then adding 1-5 wt.% of yellow rice wine or white spirit, 0.1 wt.% of thiamine and 2 wt.% of glucose into the yeast solution, and autolyzing under stirring for 24 h to obtain Candida utillis autolysate. The obtained Candida utillis autolysate is a kind of natural flavoring, and can be widely used in foods, healthcare products and industrial culture media.

The invention provides a compound probiotics preparation for geese and a method for preparing the same. The preparation is prepared from solid cultures of bacillus cereus ATCC 11778, solid cultures of lactobacillus acidophilus ATCC 4356 and solid cultures of candida utilis ATCC 22023 in a weight ratio of 1:1:1, wherein the viable count of the solid cultures of bacillus cereus is 2.5*10<11> cfu / g,the viable count of the solid cultures of lactobacillus acidophilus is 3.7*10<10> cfu / g and the viable count of the solid cultures of candida utilis is 1.05*10<10> cfu / g. The invention also provides a method for preparing the compound probiotics preparation for geese. The compound probiotics preparation is the compound bacterial agent and can obviously improve various physiologic indexes of geese; and moreover, the conditions for solid fermentation are further optimized, so that the method can greatly improve the stability and the viable count of micro-ecological preparations.

The invention provides Candida with a nematocidal activity as well as a preparation method and application of Candida and belongs to the technical field of microbial pesticides. The Candida is Candida krusei TY9 and is preserved in the typical culture preservation center in China, and the preservation number of the Candida is CCTCCNO: M2014435. The Candida krusei TY9 strain is prepared by fermentation culture of conventional liquid, is applied to prevention and control of plant-parasitic nematodes, and particularly has a good effect of preventing and controlling panagrellus redivivus and / or bursaphelenchus xylophilus. The Candida has the advantages of low cost, simple preparation process, easiness in raw material obtaining, good in effect of killing nematodes and the like, and is suitable for industrial popularization and application.