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Method for preparing selenium-enriched yeasts by using Candida utilis

A technology of Candida utilis and selenium-enriched yeast, applied in the direction of microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problem of high industrial production cost and low production cost Se-enriched yeast preparation method, low biomass of selenium-enriched yeast, etc., to achieve the effect of low cost

Inactive Publication Date: 2011-08-10
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The selenium content of the selenium-enriched yeast that this method obtains is higher, but the biomass of selenium-enriched yeast is low and the inorganic selenium that uses is many, causes industrial production cost to be higher
[0006] There is no relevant report on the preparation method of selenium-enriched yeast with low production cost, high biomass and high selenium content in the existing literature

Method used

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  • Method for preparing selenium-enriched yeasts by using Candida utilis
  • Method for preparing selenium-enriched yeasts by using Candida utilis
  • Method for preparing selenium-enriched yeasts by using Candida utilis

Examples

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preparation example Construction

[0030] 1.5 Preparation of medium

[0031] 1) Incline medium

[0032] Take 200g of peeled potatoes, cut into small pieces, add 1L of distilled water. Boil on low heat for 45-60 minutes, filter with gauze, then make up the lost water to 1L, and add sucrose: 20g; agar powder: 20g; yeast powder: 2g; peptone: 2g. Dispense it into test tubes while hot, the filling amount is 1 / 3 of the length of the test tube, and sterilize at 121°C for 20 minutes after stoppering. Before the culture medium is solidified, it is placed on a 15° slope, solidified and cultured at a constant temperature of 37°C for 24 hours to identify sterility, and stored in a 4°C refrigerator for later use.

[0033] 2) Natural medium (PS4), seed medium

[0034] Take 200g of peeled potatoes, cut into small pieces, add 1L of distilled water. Boil on low heat for 45-60 minutes, filter with gauze, then make up the lost water to 1L, and add 40g of sucrose at the same time, sterilize at 121°C for 20min, and store in a r...

Embodiment 1

[0040] The determination of embodiment 1 bacterial classification and substratum

[0041] 1. Recovery of strains

[0042] Brewer's yeast (Saccharomyces cerevisiae) and Candida utilis (Candidautilis) were respectively inoculated on the slant medium with an inoculation loop under sterile conditions, cultured at 28-30°C for 24 hours, and the strains were revived for two generations.

[0043] 2. Yeast seed culture

[0044] Fill the seed culture medium in a 250ml Erlenmeyer flask (50ml liquid volume), inoculate the revived strains into the seed culture medium respectively, and cultivate them at 150r / min at 28-30°C for 24h as seeds.

[0045] 3. Fermentation culture

[0046] 50ml of PS4, YPG and YPS liquid medium were filled in a 250ml Erlenmeyer flask respectively, and the two kinds of yeast seeds were inserted into different mediums with 10% inoculation amount, and cultured at 150r / min and 28-30°C for 72h. Harvest the bacterial liquid, centrifuge (5000r / min, 10min), discard the ...

Embodiment 2

[0054] The influence of embodiment 2pH on the growth of Candida utilis

[0055] 1. Recovery of strains

[0056] Under sterile conditions, Candida utilis was inoculated on the slant medium with an inoculation loop, cultured at 28-30°C for 24 hours, and the strains were revived for two generations.

[0057] 2. Yeast seed culture

[0058] Put the seed culture medium in a 250ml Erlenmeyer flask (the volume of liquid is 50ml), inoculate the revived strains into the seed culture medium, and cultivate them at 150r / min at 28-30°C for 24h to serve as seeds.

[0059] 3. Fermentation culture

[0060] 50ml YPS liquid culture medium is packed in the Erlenmeyer flask of 250ml, inserts Candida utilis seed in culture medium with 10% inoculum size, adjusts pH value to be respectively 4,4.5,5,5.5,6,6.5, 7. Cultivate at 150r / min, 28-30°C for 72h. Harvest the bacterial liquid, centrifuge (5000r / min, 10min), discard the supernatant to harvest the bacterial cells, dry the bacterial cells at 65-...

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Abstract

The invention discloses a method for preparing selenium-enriched yeasts by using Candida utilis, which relates to a novel method for preparing selenium-enriched yeasts. The method for preparing the selenium-enriched yeasts comprises the following steps: a, reviving Candida utilis with a collection number of CICC 1422 and preparing seeds; b, inoculating the seeds obtained by the step a onto a fermentation medium to ferment the seeds for 24 to 36 hours; c, adding selenium into fermentation liquor till the concentration of the selenium is 5 to 20mg / L, continuing fermentation for 24 to 48 hours, and collecting bacterium solution; and d, centrifuging the bacterium solution obtained by the step c, collecting thalli, drying and obtaining the selenium-enriched yeasts. In the method, the production cost, the biomass of the selenium-enriched yeasts, the selenium content in the selenium-enriched yeast can be taken into better consideration at the same time, selenium-enriched yeasts with high biomass and high selenium content can be obtained under a condition of small inorganic selenium consumption, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a new method for preparing selenium-enriched yeast, in particular to a method for preparing selenium-enriched yeast by using Candida utilis. Background technique [0002] Selenium is a recognized essential trace element, which plays an important role in the decomposition of peroxides and the scavenging of free radicals, the regulation of the body's immunity, and the antagonism of toxic elements. The current selenium supplement additives include inorganic selenium and organic selenium. Organic selenium is a better selenium supplement additive due to its strong food safety and higher bioavailability. It is a safe and effective method to convert inorganic selenium into organic selenium by biological selenium enrichment, and the production of selenium enriched yeast is relatively simple. Therefore, selenium-enriched yeast is the most commonly used selenium supplement. [0003] There are many studies on selenium-enriched yeast, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/16C12R1/72
Inventor 陈代文何军余冰
Owner SICHUAN AGRI UNIV
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