Compound probiotics preparation for geese and method for preparing same

A technology of compound probiotics and preparations, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of single effect, inability to improve poultry physiological functions, single bacterial agent, etc., to promote growth and improve immunity force, improve the effect of stability

Inactive Publication Date: 2012-09-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a compound probiotic preparation for goose, which solves the problem that the current probiotic additives for goose are mostly single bacterial agents, which have a single effect and cannot comprehensively improve the various physiological functions of poultry.

Method used

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  • Compound probiotics preparation for geese and method for preparing same
  • Compound probiotics preparation for geese and method for preparing same
  • Compound probiotics preparation for geese and method for preparing same

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The present invention will be further described below by embodiment, test example, but should not be interpreted as limitation of the present invention: the preparation of embodiment 1 bacillus cereus ATCC 11778 solid culture

[0019] 1. Activation of strains

[0020] Transfer Bacillus cereus ATCC 11778 to beef extract peptone agar slant medium (recipe), culture at 30°C for 24 hours, and set aside.

[0021] 2. First-level training

[0022] Add 10ml of sterile water to each tube to dilute the activated slant strains into a suspension, transfer to a 250ml Erlenmeyer flask containing 100ml nutrient broth medium (recipe), place in a shaker, 30°C , 130rpm shaking culture for 24h.

[0023] 3. Secondary expansion training

[0024] Take 10ml of the first-grade seed solution, put them into a 250ml Erlenmeyer flask filled with 115ml of nutrient broth medium (recipe), place them in a shaker, and cultivate them with shaking at 30°C and 130rpm for 24h.

[0025] 4. Solid fermenta...

Embodiment 2

[0029] The preparation of embodiment 2 Lactobacillus acidophilus ATCC 4356 solid culture

[0030] 1. Activation of strains

[0031] Transfer Lactobacillus acidophilus ATCC 4356 to MRS agar slant medium (recipe), culture in an anaerobic incubator at 30°C for 24 hours, and set aside.

[0032] 2. First-level training

[0033] Add 10ml of sterile water to each tube to dilute the activated slant bacteria into a suspension, transfer it to a 250ml Erlenmeyer flask containing 100ml of nutrient broth medium (recipe), put it in an anaerobic shaker, 30°C, 130rpm shaking culture for 24h.

[0034] 3. Secondary expansion training

[0035] Take 10ml of the first-grade seed solution, put them into a 250ml Erlenmeyer flask filled with 115ml of MRS medium (recipe), put them in an anaerobic shaker, and cultivate them with shaking at 30°C and 130rpm for 24h.

[0036] 4. Solid fermentation

[0037] According to the weight ratio of 48.875% rice bran, 10% corn, 20% soybean meal, DDGS 20%, 0.1% di...

Embodiment 3

[0040] The preparation of embodiment 3 Candida utilis ATCC 22023 solid culture

[0041] 1. Activation of strains

[0042] Transfer Candida utilis ATCC 22023 to PDA agar slant medium (recipe), culture at 30°C for 24 hours, and set aside.

[0043] 2. First-level training

[0044] Add 10ml of sterile water to each tube to dilute the activated slant strains into a suspension, transfer to a 250ml Erlenmeyer flask containing 100ml nutrient broth medium (recipe), place in a shaker, 30°C , 130rpm shaking culture for 24h.

[0045] 3. Secondary expansion training

[0046] Take 10ml of the first-grade seed solution, put them into a 250ml Erlenmeyer flask filled with 115ml of PDA medium (recipe), put them in a shaker, and cultivate them with shaking at 30°C and 160rpm for 24h.

[0047] 4. Solid fermentation

[0048] According to the weight ratio of 42.4% rice bran, 15% corn flour, 5% rice husk, 15% soybean meal powder, 22.5% DDGS, 0.1% potassium dihydrogen phosphate to prepare fermen...

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Abstract

The invention provides a compound probiotics preparation for geese and a method for preparing the same. The preparation is prepared from solid cultures of bacillus cereus ATCC 11778, solid cultures of lactobacillus acidophilus ATCC 4356 and solid cultures of candida utilis ATCC 22023 in a weight ratio of 1:1:1, wherein the viable count of the solid cultures of bacillus cereus is 2.5*10<11> cfu / g,the viable count of the solid cultures of lactobacillus acidophilus is 3.7*10<10> cfu / g and the viable count of the solid cultures of candida utilis is 1.05*10<10> cfu / g. The invention also provides a method for preparing the compound probiotics preparation for geese. The compound probiotics preparation is the compound bacterial agent and can obviously improve various physiologic indexes of geese; and moreover, the conditions for solid fermentation are further optimized, so that the method can greatly improve the stability and the viable count of micro-ecological preparations.

Description

technical field [0001] The invention belongs to the field of microbial feed additives, and relates to a compound probiotic preparation for goose and a preparation method thereof. Background technique [0002] At present, the problem of antibiotic residues in animal products and the environment has been paid more and more attention. In order to fundamentally remove antibiotics from compound feed, scientists in related fields around the world are exploring alternatives to antibiotics, trying to find a drug-free, pollution-free, residue-free, non-toxic and side-effect-free, safe antibiotic New feed additives. It is in this situation that probiotics are used in feed additives. After the glorious era of antibiotics, there will be a new era of microecological preparations. [0003] In recent years, the development and application of probiotics has played an active role in promoting the development of animal husbandry in my country, and has great potential. It not only has the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/16C12N1/20C12R1/72C12R1/23C12R1/085
Inventor 许丽王秋菊庞晖金慧然
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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