Novel EM (effective microbiota) stock solution and use method thereof

Abstract

The invention discloses a novel EM (effective microbiota) stock solution and a use method thereof. The stock solution consists of a photosynthetic bacteria group, a lactic acid bacteria group, a saccharomycetes group, an actinomycetes group, a filamentous bacteria group of a fermentation system, bifidobacteria and bacillus. The method can be applied to the fields of cultivation, aquatic products, planting, environmental protection and the like; the organic fertilizer has the characteristics of preventing diseases, promoting growth of animals and plants, improving vitality, shortening the growth cycle and improving the yield in agriculture, and has the effects of inhibiting bacteria, deodorizing and improving the soil environment in the fields of environmental protection and environment; effective strains of the EM stock solution have extremely high deodorization and decomposition capabilities, deodorization work can be completed within 20 seconds, and decomposition work of harmful microorganisms and harmful substances can be achieved within 3 minutes; moreover, the environment resistance is extremely high, 95% of activity (30 minutes) can be kept at the high temperature of 115 DEG C, and the activity is still enough at the low temperature of-27 DEG C.

Description

Technical Field

[0001] The invention belongs to the technical field of effective microorganisms, and relates to a novel EM (effective microorganism) stock solution and a use method thereof. Background Art

[0002] EM is the abbreviation of effective microorganisms, which is composed of photosynthetic bacteria, lactic acid bacteria, yeast and other microorganisms. EM technology is the most widely used bioengineering technology in the world. Compared with general biological preparations, it has the advantages of complex structure, stable performance and complete functions. EM bacteria technology can be used in human home health care, industry, agriculture, animal husbandry and environmental protection; however, the EM stock solution in the existing technology requires different strain ratios for different application fields; in actual use, a single strain ratio can only be used in a single field, and cannot be used in multiple fields. Summary of the invention

[0003] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0004] A new type of EM (effective microbial community) stock solution, composed of photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, filamentous bacteria of the fermentation system, bifidobacteria and bacillus.

[0005] A novel method for using EM (effective microbiome) stock solution, including the following usage:

[0006] Direct dilution method: According to the purpose of use, the EM stock solution is directly diluted with water in a specific ratio and directly used in work including but not limited to: spraying on crop leaves, irrigation of farmland, drinking for livestock and poultry, environmental deodorization, etc.

[0007] Preparation of active liquid: dilute the EM stock solution and an equal amount of molasses with water in different proportions of 10-50 times, and place in a sealed container for anaerobic fermentation; when the pH drops to about 3.5 and there is a mellow fermentation sour taste, the fermentation is complete; when using, dilute the active liquid with water in a specific proportion.

[0008] Preparation of EM fermented feed: for every 100 portions of solid feed, use 0.1-0.3 portions of EM stock solution and an equal amount of molasses or brown sugar, dilute with 20-30 portions of water and mix evenly into the solid feed; seal and ferment; when the mellow aroma of the koji appears, the fermentation is complete.

[0009] Making EM compost: Cow dung, pig dung and other livestock manure are mixed with water-absorbing materials such as straw, rice husks and sawdust; dissolve EM stock solution and molasses in water, spray evenly on the cow dung mixture, and turn it over while spraying. After the dung pile is piled to a certain height, cover it with plastic film, use straw curtains to block light, and ferment. During the fermentation process, turn the pile over appropriately. When white mycelium appears on the surface of the dung pile and there is a fragrance of distiller's yeast, the fermentation is successful.

[0010] Make EM insect repellent: prepare EM stock solution, molasses, vinegar, liquor (above 30°), and water in a ratio of 1:1:1:1:10; mix the EM stock solution and molasses, put them into a container and cover tightly, ferment for 2-3 days, after gas is produced and swells, open the lid to release gas, add vinegar and liquor in the same amount as the EM stock solution, mix well, cover tightly, and ferment for about two more weeks;

[0011] During this period, if the container swells due to gas production, it is necessary to release the gas in time and cover it tightly immediately. When gas is no longer produced and the sweet and sour ester aroma can be smelled, it indicates that the fermentation is successful.

[0012] When using, dilute it at a standard dilution multiple of 1000-500.

[0013] As a further solution of the present invention: during the dilution work, water without chlorine is used as the dilution water; therefore, the dilution water is preferably well water. If tap water is used, it should be left for a day and night to allow the chlorine to dissipate before use.

[0014] Beneficial effects of the present invention: The novel EM stock solution is composed of photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, filamentous bacteria of the fermentation system, bifidobacteria and bacillus; it can be applied in the fields of breeding, aquaculture, planting and environmental protection;

[0015] It can be used in the breeding industry and is applicable to most livestock and poultry and special breeding. It can improve feed utilization and reduce costs. It can also improve reproduction rate and survival rate, promote animal growth, increase daily weight gain, shorten feeding time, effectively improve the quality and yield of livestock and poultry meat and eggs, reduce or even use antibiotics, and produce green livestock and poultry products.

[0016] It can be used in the planting industry and can be applied to the planting of various crops. It can enhance plant metabolism, promote photosynthesis, strengthen the leaf protective film, and promote the growth of the root system. At the same time, it can produce antibacterial substances, inhibit the reproduction of harmful microorganisms, and ensure stable and high yields of crops.

[0017] At the same time, it can also improve the planting soil, increase soil organic matter to improve soil fertility, thoroughly improve soil properties, eliminate soil pollution, etc.;

[0018] When used in aquaculture, it can control the content of harmful substances such as ammonia and hydrogen sulfide in the water environment, and achieve the effect of regulating the microbial ecological structure of the breeding environment; enhance the immune function of aquatic animals, prevent diseases, improve health, reduce morbidity and mortality, thereby improving the vitality of aquaculture, shortening the growth cycle, and increasing production;

[0019] When used in environmental protection work, it can promote the decomposition of organic pollutants, reduce BOD and COD, and achieve the goal of purifying water quality; it can improve the purification capacity of the sewage treatment system, reduce sludge generation, and reduce costs;

[0020] The effective bacteria of EM stock solution have strong deodorizing and decomposing abilities, and can complete deodorizing work within 20 seconds and decompose harmful microorganisms and harmful substances within 3 minutes. It also has strong environmental resistance, and can maintain 95% activity (30 minutes) at high temperatures of 115°C, and still have sufficient activity at low temperatures of -27°C.

[0021] Among them, the photosynthetic bacteria are used to provide the nutrients required by the original liquid bacteria. The metabolites they produce can be directly absorbed by plants and can also become nutrients for the reproduction of other microbial bacteria.

[0022] Lactic acid bacteria can form lactic acid with strong bactericidal ability, which can effectively inhibit the activities of harmful microorganisms and rapidly decompose organic matter;

[0023] Yeast flora can promote root growth and cell division as an activated substance; it provides important nutrition guarantee for the matrix required for the proliferation of other effective microorganisms;

[0024] Actinomycetes can produce various antibiotics, vitamins and enzymes, which directly inhibit pathogens and are easily absorbed by animals and plants, thus enhancing their resistance and immunity to various diseases.

[0025] The filamentous flora of the fermentation system can have a good effect on the formation of esters in the soil, prevent the occurrence of maggots and other pests, and eliminate bad odors;

[0026] Bifidobacterium can synthesize multiple vitamins, balance the flora in the stock solution, and can also grow and reproduce in the intestines of animals, competing with harmful bacteria for nutrients and maintaining the balance of intestinal microorganisms;

[0027] Bacillus can regulate the balance of animal intestinal flora, improve the intestinal microecological environment, and effectively maintain the health of animal bodies. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present application are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. It should be understood that the present application is not limited to the example embodiments disclosed herein. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0029] In the embodiment of the present invention, a novel EM (effective microorganism) stock solution is composed of photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, filamentous bacteria of fermentation system, bifidobacteria and bacillus.

[0030] Photosynthetic bacteria (aerobic and anaerobic): photosynthetic bacteria and cyanobacteria are independent nutritional microorganisms. The bacteria themselves contain more than 60% protein and are rich in various vitamins. They also contain coenzyme Q10, antiviral substances and growth-promoting factors. The metabolites of photosynthetic bacteria can be directly absorbed by plants and can also become nutrients for the reproduction of other microorganisms. If photosynthetic bacteria proliferate, other beneficial microorganisms will also proliferate.

[0031] Lactic acid bacteria group (anaerobic): dominated by Lactobacillus acidophilus, which forms lactic acid by absorbing sugars produced by photosynthetic bacteria and yeast; lactic acid has a strong bactericidal ability and can effectively inhibit the activity of harmful microorganisms and the rapid decomposition of organic matter; lactic acid bacteria can decompose lignin and cellulose that are difficult to decompose under normal conditions, and ferment and decompose organic matter; lactic acid bacteria can also inhibit the proliferation of pathogenic bacteria caused by continuous cropping problems.

[0032] Yeast flora (aerobic): can utilize secretions produced by plant roots, amino acids, sugars and other organic substances synthesized by photosynthetic flora to produce fermentation power, and synthesize activating substances that promote root growth and cell division; provide important nutrient guarantee for the matrix (food) required for the proliferation of other effective microorganisms (such as lactic acid bacteria and actinomycetes); in addition, the single-cell protein produced by yeast is an indispensable nutrient for animals.

[0033] Actinomycetes (aerobic): can obtain amino acids and nitrogen from photosynthetic bacteria as matrix, produce various antibiotics, vitamins and enzymes, and directly inhibit pathogens; actinomycetes inhibit the proliferation of harmful molds and bacteria by obtaining the matrix they need for proliferation in advance, and create a living environment for the proliferation of other beneficial microorganisms; the bactericidal effect of the mixture of actinomycetes and photosynthetic bacteria is much greater than the lethality of actinomycetes alone. It has a degrading effect on difficult-to-decompose substances such as lignin, cellulose, chitin, etc., and is easily absorbed by animals and plants, enhancing the resistance and immunity of animals and plants to various diseases; actinomycetes also promote the proliferation of nitrogen-fixing bacteria and VA mycorrhizal bacteria.

[0034] Filamentous flora of the fermentation system (anaerobic): Mainly composed of Aspergillus used in alcohol fermentation, it can coexist with other microorganisms, especially has a good effect on the formation of esters in the soil; because of its strong alcohol production ability, it can prevent the occurrence of maggots and other pests, and can eliminate odors.

[0035] Bifidobacterium: It can synthesize multiple vitamins and balance the flora in the stock solution; it can also grow and reproduce in the intestines of animals, compete with harmful bacteria for nutrients, and maintain the balance of intestinal microorganisms; biologists found that some substances can significantly promote the growth of bifidobacteria when studying in vitro culture of intestinal physiological bacteria, so they are called bifidobacteria factors. These substances include: bifidobacteria factor I (human colostrum), bifidobacteria factor II (peptides and hypoxanthine), carrot bifidobacteria factor and oligosaccharide bifidobacteria factor; oligosaccharide bifidobacteria factors are different types of oligosaccharides, which cannot be used by the body and some harmful bacteria, but can promote the growth of bifidobacteria and some lactic acid bacteria.

[0036] Bacillus: A microecological preparation produced with Bacillus subtilis as the strain, using the domestically pioneered adsorption carrier fermentation technology and low-temperature drying technology; it can regulate the balance of animal intestinal flora, improve the intestinal microecological environment, effectively maintain the health of the animal body, improve the digestion and utilization rate of feed by animals, and improve the body's immunity and anti-stress ability; it reduces the use of or uses less antibiotics, and is a natural, economical green feed additive product; the active substances such as subtilis, polymyxin, nystatin, and gramicidin produced by Bacillus subtilis during its growth process have a significant inhibitory effect on pathogenic bacteria or conditional pathogens of endogenous infection; Bacillus subtilis can quickly consume The free oxygen in the digestive tract environment forms a hypoxic environment in the intestine, promotes the growth of beneficial anaerobic bacteria, and produces organic acids such as lactic acid, which lowers the pH value of the intestine and indirectly inhibits the growth of other pathogenic bacteria; Bacillus subtilis can stimulate the growth and development of animal immune organs, activate lymphocytes, increase the levels of immunoglobulins and antibodies, enhance cellular immunity and humoral immunity, and improve group immunity; Bacillus subtilis can synthesize digestive enzymes such as protease, amylase, lipase, cellulase, etc., which work together with endogenous enzymes in the digestive tract to improve feed digestibility; Bacillus subtilis can synthesize a variety of vitamins and increase the activity of interferon and macrophages in animals.

[0037] In an embodiment of the present invention, a method for using the novel EM (effective microorganism) stock solution is also provided, the method mainly comprising: direct dilution method, making active solution, making EM fermented feed, making EM compost and making EM insect repellent;

[0038] Direct dilution method: according to the purpose of use, the EM stock solution is directly diluted with water in a specific ratio and directly used in work including but not limited to: spraying on crop leaves, irrigation of farmland, drinking water for livestock and poultry, environmental deodorization, etc. (the dilution ratio varies for different fields);

[0039] Preparation of active liquid: dilute the EM stock solution with an equal amount of molasses (brown sugar can also be used as a substitute) with water in different proportions of 10-50 times (molasses or brown sugar must be dissolved in hot water first), and place it in a sealed container for anaerobic fermentation (fermentation environment temperature 20-35°C); during the fermentation process, the container swells due to gas production, so it is necessary to release the gas in time; when the pH drops to about 3.5 and there is a mellow fermentation sour taste, the fermentation work is completed; when using, dilute the active liquid with water in a specific proportion.

[0040] Preparation of EM fermented feed: for every 100 parts of solid feed (livestock and poultry feed, aquatic feed, etc.), use 0.1-0.3 parts of EM stock solution and an equal amount of molasses or brown sugar, dilute with 20-30 parts of water and mix evenly into the solid feed; seal and ferment (fermentation environment temperature is above 20°C); wait for the mellow aroma of the wine koji to appear, and the fermentation work is completed;

[0041] In addition, when mixing with solid feed, the moisture content needs to be controlled to a level where it can be formed into a ball when squeezed and falls apart when touched. Too much moisture will cause it to spoil easily, while too little moisture will lead to insufficient fermentation.

[0042] Making EM compost: Cow dung, pig dung and other livestock manure (or fermented products such as soybean meal, rapeseed cake, distiller's grains can also be used to make EM compost in this way) are mixed with water-absorbing materials such as straw, rice husk, sawdust, etc.; ferment and make compost in the following way: Take cow dung as an example: 1000kg of cow dung, 100kg of straw powder or rice husk, sawdust and cow dung are mixed evenly; 2-3kg of EM stock solution, 2-3kg of molasses, 20-30% of water (the amount of water is increased or decreased depending on the dryness and humidity), dissolve the EM stock solution and molasses in water, spray evenly on the cow dung mixture, turn it while spraying, and pile it into a dung pile with a height of 40CM and a width of about 100CM, cover it with plastic film, use straw curtains or the like to properly block light, and ferment; and during the fermentation process, turn the pile appropriately, and when white mycelium appears on the surface of the dung pile and there is a fragrance of distiller's yeast, the fermentation is successful.

[0043] Make EM insect repellent: prepare EM stock solution, molasses, vinegar, liquor (above 30°), and water in a ratio of 1:1:1:1:10; mix the EM stock solution and molasses, put them into a container and cover tightly, ferment for 2-3 days, after gas is produced and swells, open the lid to release gas, add vinegar and liquor in the same amount as the EM stock solution, mix well, cover tightly, and ferment for about two more weeks;

[0044] During this period, if the container swells due to gas production, it is necessary to release the gas in time and cover it tightly immediately. When gas is no longer produced and the sweet and sour ester aroma can be smelled, it indicates that the fermentation is successful.

[0045] When using, dilute it at a standard dilution multiple of 1000-500.

[0046] Furthermore, in the dilution work of the above-mentioned usage method, it is preferred to use water without chlorine as the dilution water; therefore, the dilution water is preferably well water. If tap water is used, it should be left for a day and night to allow the chlorine to dissipate before use.

[0047] In the embodiment of the present invention, the application of the novel EM (effective microorganism) stock solution in actual breeding, agriculture, aquaculture and environmental protection based on the above-mentioned use method is also provided, specifically including:

[0048] How to use EM stock solution in aquaculture:

[0049] 1. Drinking: dilute the solution by direct dilution at a ratio of 1 (stock solution): 200-500 (water) and feed to the baby;

[0050] 2. Make EM fermented feed: Seal and ferment at a ratio of 1 (stock solution): 500 (feed), with 60% water;

[0051] 3. Make EM compost: Seal and ferment at a ratio of 1 (stock solution): 100-300 (manure), with 60% water content;

[0052] 4. Make the fermentation bed: Select materials: 60% sawdust, 30% rice bran, 10% soil, 0.3% salt. One square meter of fermentation bed requires 1-2 kg of stock solution. Fermentation bed thickness: (livestock: 60-90 cm, poultry: 30-50 cm)

[0053] 5. Deodorization and disinfection should be carried out by spraying the pens regularly at a ratio of 1:500-1000 using the direct dilution method.

[0054] The role of EM bacteria in breeding:

[0055] It improves feed conversion rate, promotes animal growth and development, improves meat and egg quality, inhibits and eliminates odor in the breeding house, improves the internal and external environment of animals, kills pathogenic microorganisms, inhibits the growth of putrefactive bacteria, improves animal disease resistance and fertility, greatly reduces feeding costs, and produces high-quality, pollution-free, healthy and green food.

[0056] 1. Improve feed conversion rate and reduce costs. The macromolecular organic matter (lignin, chitin, etc.) in feed fermented or treated by EM is decomposed into small molecular organic matter (sugars, enzyme acids, etc.). In addition, the existence of the bacteria itself and the active enzymes secreted (synthesized) by it greatly improves the nutritional value of the feed. The total amount of 18 kinds of amino acids is significantly increased, and the increase in different nutrients is between 10-28%. The palatability is better, and the investment cost of EM is low (1 kg can ferment 500-1000 kg of feed).

[0057] 2. It has a unique preventive and therapeutic effect on epidemic diseases. It can reduce or even eliminate the use of veterinary drugs and reduce the incidence of livestock and poultry by more than 70%. It can effectively prevent and treat yellow diarrhea, white diarrhea, gastroenteritis, gastric indigestion, ruminant forestomachal achalasia, rumen bloat, Newcastle disease, coccidiosis, and sudden death of livestock and poultry, and can prevent and treat various ulcers, stomatitis, obstetric inflammation, and cold and fever.

[0058] 3. Promote growth, increase daily weight gain, and shorten feeding time. For example, the daily weight gain of suckling pigs can be increased by more than 40% compared with conventional feeding.

[0059] 4. Significantly improve the reproduction rate. For example, the litter rate of sows can be increased by 30-50% (EM feed should be fed at least 20 days before mating), laying hens can lay eggs about 10 days earlier, the egg production rate can be increased by more than 15%, and the peak egg production period can be extended by two months.

[0060] 5. Eliminate the stench of the barns. The ammonia emission is reduced to about 250PPM (ammonia removal rate reaches 69.7%), gradually eliminating mosquitoes and flies, purifying the environment and reducing the occurrence of diseases.

[0061] 6. Excellent detoxification performance. EM can detoxify 95% of the isocyanate of rapeseed worms. Strictly treating moldy feed with EM can achieve the effect of making the feed taste as good as new.

[0062] 7. Comprehensively improve the quality of meat, eggs and milk, and produce fresh, tender and non-smelly pure natural green food. For example, the National Health Ministry's Food Hygiene Supervision and Inspection Institute found that after testing EM eggs, the protein content increased by 5.56%, the fat decreased by 73.6%, and the cholesterol decreased by 82.2%. There were no chemical hormone components, and the egg yolk was deep and the egg white was viscous.

[0063] How to use EM stock solution in agriculture:

[0064] 1. Land preparation:

[0065] Paddy fields: During spring ploughing, for fields with fertilizer grass and rice straw returned to the fields, spray 1000 times diluted EM stock solution per mu for fermentation (about 1-2 kg of EM stock solution is needed); for fields without fertilizer grass and rice straw returned to the fields, apply 50-100 kg of EM compost per mu.

[0066] Dry land: When preparing the land, apply 50-100 kg of EM compost per mu, till it into the soil about 20 cm, and evenly spray it with 500-1000 times diluted EM stock solution.

[0067] 2. Seed soaking: Soak the seeds in 200 times diluted EM stock solution for 2-4 hours before sowing (adjust the time according to the seed size and seed coat hardness);

[0068] 3. Seedling stage: spray 3-5 times with 1000 dilution of EM.

[0069] 4. Growing period: Use 500-1000 times diluted EM stock solution to spray the leaves (front and back sides) or water the roots. Generally, spray once every 1-2 months, and apply fermented compost or manure water depending on the growth of the crop.

[0070] 5. Pest and disease prevention: Starting from the seedling stage, you can spray 500 times diluted EM insect repellent (foliar spray). Spraying EM active liquid once a month will have a better effect.

[0071] 6. Vegetables: Apply EM compost as base fertilizer in vegetable fields or greenhouses, soak seeds in 200 times diluted EM, spray 500-1000 times diluted EM on leaves during the growth period once every 1-2 weeks, and use 1000 times diluted EM for watering. You can also spray EM insect repellent (500 times diluted) as needed.

[0072] 7. Fruit trees: When preparing the land in the nursery, EM fermented compost can be applied as base fertilizer, about 100 kg per mu. When applying, it can be evenly spread and shallowly ploughed, or it can be buried in holes around the plants, with a depth of about 15-20 cm. Seeds can be soaked in 200 times diluted EM, and 500-100 times diluted EM can be sprayed regularly during the seedling period and the entire growth period, and EM insect repellent (500 times diluted) can be sprayed. When cutting or dividing, soak the incision in 500-1000 times diluted EM for 30 minutes, and plant after drying.

[0073] The role of EM bacteria in agriculture:

[0074] It promotes the decomposition of organic matter and photosynthesis in the soil, improves the physical properties of the soil to make it loose, breathable and permeable, while inhibiting the reproduction of harmful microorganisms, reducing or even eliminating the use of chemical fertilizers and pesticides, and producing truly green food.

[0075] It can improve soil quality, reduce pests and diseases, reduce costs, and achieve high quality and high yield. For example, it has the unique effects of green leaves and fresh flowers, early flowering and flower preservation for flowers, excellent mushroom growth effect for edible fungi, and spraying on leafy vegetables can increase the yield by 30%.

[0076] 1. Enhance plant metabolism, promote photosynthesis and strengthen leaf protective film, resist pathogens and promote the development of root system.

[0077] 2. Produce antibacterial substances to inhibit the reproduction of harmful microorganisms and produce beneficial substances to prevent and control various diseases of crops.

[0078] 3. Beneficial bacteria coexist and colonize with actinomycetes in the soil to form a good crop growth environment, improve soil fertility, and thoroughly improve soil properties, ultimately achieving green organic agricultural products, no-till operations, and sustainable development.

[0079] How to use EM stock solution in aquaculture:

[0080] 1. Breeding pond:

[0081] ① Pretreatment: One week before releasing water, spread EM solid fermentation material into the dry pond, about 30 kg per mu.

[0082] ② Water treatment: 3-10 days before stocking, sprinkle the water surface with 100 times diluted EM solution, about 2-4 kg of EM solution per mu (depending on the depth of the fish pond). After that, sprinkle once a month depending on the situation (the amount can be reduced appropriately).

[0083] ③ Feed processing: ferment the feed with EM (see above for the method), mix the fermented feed into ordinary feed at a ratio of 1-5% and feed it. You can also use 100 times EM dilution to spray directly on the feed (wetness as the degree) and then feed it, or use 300 times dilution to evenly mix the feed and then feed it.

[0084] 2. Cage fish farming:

[0085] After the feed is fermented with EM, it is mixed into ordinary feed at a ratio of 10% to 30% and fed.

[0086] Soak a sponge or other absorbent material in EM stock solution and 10-fold dilution of molasses for 24 hours, then hang it in the net cage to allow the EM to spread naturally through the movement of the fish. Replace the hanging material once a week.

[0087] 3. Special aquatic products (soft-shelled turtle, prawn):

[0088] ① Turtle: Mix the EM diluted solution evenly into the feed and then feed it (approximately 3 ml of EM original solution is used for every kilogram of feed).

[0089] After each water change, sprinkle EM dilution solution to make the EM concentration in the pool water reach 10ppm.

[0090] ②Penaeans: Release EM into the pond water: in the early stage (body length 0.5-2.5cm), release concentration is 2-3ppm of the pond water, once every 4 days; in the late stage (body length over 2.5cm), release concentration is 4-6ppm of the pond water, once every 3 days.

[0091] Add EM to feed: Mix EM stock solution into feed at a ratio of 0.2% (dilute first and then mix in).

[0092] The role of EM bacteria in aquaculture:

[0093] Improve health and reduce morbidity and mortality;

[0094] Promote growth, weight gain and increase yield;

[0095] Improve meat quality;

[0096] Eliminate harmful germs, purify water quality, improve the environment, and extend the water change interval.

[0097] Reduce the use of antibiotics.

[0098] How to use EM stock solution in environmental protection

[0099] 1. Water treatment

[0100] ① Make EM active liquid: Since the amount of EM used in environmental protection projects is large, in order to reduce costs, the EM stock solution can be expanded and cultured into active liquid at the treatment site. If the treated sewage is used for culture, the purpose of domestication can also be achieved, making EM more adaptable to sewage. Culture method (reference ratio): 1% EM stock solution, 1% molasses, 10% sewage, after filling with clean water, culture in a closed environment above 25℃ for about one week, the pH reaches below 4.0, the sewage odor disappears and there is a fermented mellow sour taste, which is successful (because the sewage composition, concentration, etc. vary greatly, the specific domestication culture method should be tested on site).

[0101] ② Sewage treatment plant: EM active liquid is placed at a ratio of 1 / 1000-1 / 2000 of the sewage volume. The placement site should be set at the initial stage of the treatment system as much as possible, and the sewage should flow in evenly and continuously according to the sewage treatment volume. 5% of the discharged treated water can be returned to the primary stage every day. When using EM for treatment, intermittent aeration method can be used. Effect: Reduce sludge and reduce on-site odor.

[0102] ③Treatment of river and lake water quality: Sprinkle EM active liquid directly on the water surface, about 200-400 liters per mu. If there is a lot of silt at the bottom, the EM active liquid can be directly injected into the bottom mud when the pond is dredged. In addition, the bottom mud can be added to EM and dried to make EM mud balls, which are thrown into the water, one per square meter. Broken bricks can also be soaked in EM liquid, fully absorbed, and then thrown into the water. In small rivers, you can also use Miscanthus, thin bamboo leaves, rice straw, etc., soak them in EM liquid, put them in sacks or straw bags, and place them upstream. (For seriously polluted rivers and lakes, it is necessary to intercept the pollution or treat the pollution source at the source).

[0103] EM mud ball production method

[0104] (1) Mix dry soil or semi-dry soil (crushed) and fermented feed in a ratio of 5:1.

[0105] (2) Pour in fresh EM active liquid (enlargement medium), stirring while pouring. The amount of active liquid used (humidity) should be based on the amount that can be easily kneaded into a mud ball. The diameter of the mud ball is about 8 to 12 cm.

[0106] (3) Place in a cool place for natural fermentation. To prevent the water from evaporating too quickly, cover it with wet newspaper. If the surface of the newspaper is too dry, spray it with EM active liquid to make it slightly moist (do not spray too much).

[0107] (4) After fermentation for 4 days to 1 week, when white hair grows on the surface of the mud ball, move it to the sun to dry. The EM mud ball is now made.

[0108] 2. Waste Disposal

[0109] Urban garbage compression transfer stations can be sprayed evenly with 20-50 times diluted EM active liquid, and each batch of garbage sent in should be sprayed (preferably in mist spray). Large landfills can be sprayed with a sprinkler truck. Effect: Reduce or eliminate odor, inhibit the reproduction of spoilage bacteria, and reduce mosquitoes and flies.

[0110] Kitchen waste from households and restaurants: Leftovers, vegetable leaves, fruit peels and other organic kitchen waste can be processed with EM and turned into compost. The specific method is: first turn a plastic basket upside down in the trash can, and then put in a garbage bag (open some small holes in the bottom of the bag in advance to facilitate liquid seepage). Before the garbage enters, sprinkle a layer of EM solid fermentation material. For each batch of garbage entering, sprinkle an appropriate amount of EM solid fermentation material until the bucket is full (the trash can should be kept covered during the process). After being sealed for one week, it will become EM compost (with the aroma of wine koji and white mycelium on the surface). Take out the garbage bag and pour out the leachate at the bottom of the bucket. (If the fermentation is unsuccessful and the garbage stinks, the amount of EM solid fermentation material put in can be increased).

[0111] 3. Others (household environmental protection)

[0112] You can often spray some EM liquid (diluted 50-100 times) on the sewers and toilets in your home to deodorize and reduce mosquitoes and flies.

[0113] When washing vegetables, fruits and melons, add 1-2 bottle caps of EM solution and soak for 1-2 hours. This can help decompose residual pesticides.

[0114] To make fermented rice water: put rice water (the thicker the better) into a 1.5-liter beverage bottle, add 2 spoons of EM stock solution and an equal amount of brown sugar, shake well and ferment in a sealed container at a temperature above 25°C for 3-5 days. When the white rice water turns light brown and has a mellow fermentation smell, the fermentation is successful. It can be used for deodorizing sewers and toilets, diluting 500 times to water flowers, etc.

[0115] The role of EM bacteria in environmental protection:

[0116] It promotes the decomposition of organic pollutants, reduces pollutants such as BOD and COD, and has low cost.

[0117] It should also be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of more restrictions, an element defined by the sentence "comprises a ..." does not exclude the presence of other identical elements in the process, method, article or device including the element.

[0118] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A novel EM (effective microbial community) stock solution, characterized in that: It is composed of photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, filamentous bacteria of fermentation system, bifidobacteria and bacillus; Photosynthetic bacteria: photosynthetic bacteria and cyanobacteria are independent nutrient microorganisms; the metabolites of photosynthetic bacteria can be directly absorbed by plants and can also become nutrients for the reproduction of other microorganisms; Lactic acid bacteria: Lactobacillus acidophilus is the dominant species, which forms lactic acid by absorbing sugars produced by photosynthetic bacteria and yeast. Lactic acid has a strong bactericidal ability and can effectively inhibit the activity of harmful microorganisms and the rapid decomposition of organic matter. Yeast flora: can use secretions produced by plant roots, amino acids, sugars and other organic substances synthesized by photosynthetic flora to produce fermentation power, and synthesize activating substances that promote root growth and cell division; Actinomycetes: can obtain amino acids and nitrogen from photosynthetic bacteria as a matrix, produce various antibiotics, vitamins and enzymes, and directly inhibit pathogens; The filamentous flora of the fermentation system: mainly composed of Aspergillus used in alcohol fermentation, it can coexist with other microorganisms, especially has a good effect on the formation of esters in the soil; it can prevent the occurrence of maggots and other pests, and can eliminate bad odors; Bifidobacterium: It can synthesize multiple vitamins and balance the flora in the stock solution; it can also grow and multiply in the intestines of animals, compete with harmful bacteria for nutrients, and maintain the balance of intestinal microorganisms; Bacillus: Bacillus subtilis is used as the strain; it can regulate the balance of animal intestinal flora, improve the intestinal microecological environment, and effectively maintain the health of the animal body.

2. A method for using a novel EM (effective microbial community) stock solution, used for the use of the novel EM (effective microbial community) stock solution method according to claim 1, characterized in that: These include: Direct dilution method: According to the purpose of use, the EM stock solution is directly diluted with water in a specific ratio and directly used in work including but not limited to: foliar spraying of crops, irrigation of farmland, drinking water for livestock and poultry, environmental deodorization, etc.

3. A method for using a novel EM (effective microorganism) stock solution, used for the use of the novel EM (effective microorganism) stock solution method according to claim 1, characterized in that: These include: Preparation of active liquid: dilute the EM stock solution and an equal amount of molasses with water in different proportions of 10-50 times, and place in a sealed container for anaerobic fermentation; when the pH drops to about 3.5 and there is a mellow fermentation sour taste, the fermentation is complete; when using, dilute the active liquid with water in a specific proportion.

4. A method for using a novel EM (effective microbial community) stock solution, used for the use of the novel EM (effective microbial community) stock solution method according to claim 1, characterized in that: These include: Preparation of EM fermented feed: for every 100 portions of solid feed, use 0.1-0.3 portions of EM stock solution and an equal amount of molasses or brown sugar, dilute with 20-30 portions of water and mix evenly into the solid feed; seal and ferment; when the mellow aroma of the koji appears, the fermentation is complete.

5. A method for using a novel EM (effective microorganism) stock solution, used for the use of the novel EM (effective microorganism) stock solution method according to claim 1, characterized in that: These include: Making EM compost: Cow dung, pig dung and other livestock manure are mixed with water-absorbing materials such as straw, rice husks and sawdust; dissolve EM stock solution and molasses in water, spray evenly on the cow dung mixture, and turn it over while spraying. After the dung pile is piled to a certain height, cover it with plastic film, use straw curtains to block light, and ferment. During the fermentation process, turn the pile over appropriately. When white mycelium appears on the surface of the dung pile and there is a fragrance of distiller's yeast, the fermentation is successful.

6. A method for using a novel EM (effective microorganism) stock solution, used for the use of the novel EM (effective microorganism) stock solution method according to claim 1, characterized in that: These include: Make EM insect repellent: prepare EM stock solution, molasses, vinegar, liquor (above 30°), and water in a ratio of 1:1:1:1:10; mix the EM stock solution and molasses, put them into a container and cover tightly, ferment for 2-3 days, after gas is produced and swells, open the lid to release gas, add vinegar and liquor in the same amount as the EM stock solution, mix well, cover tightly, and ferment for about two more weeks; During this period, if the container swells due to gas production, it is necessary to release the gas in time and cover it tightly immediately. When the gas stops being produced and the sweet and sour ester aroma can be smelled, it indicates that the fermentation is successful. When using, dilute it at a standard dilution multiple of 1000-500.

7. A method for using a novel EM (effective microbial community) stock solution according to any one of claims 2, 3 or 6, characterized in that: During the dilution work, use water that does not contain chlorine as the dilution water; therefore, the best dilution water is well water. If tap water is used, it should be left for a day and night to allow the chlorine to dissipate before use.