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Ecological restoration microbial inoculum capable of inhibiting mildews and degrading mycotoxin

A technology of mycotoxins and ecological restoration, applied in the directions of biocides, disinfectants, fungicides, etc., can solve the problems of inability to decompose or promote the decomposition of mycotoxins, and inability to degrade mycotoxins, and achieve the effect of high activity and high density

Inactive Publication Date: 2015-04-29
JILIN JIUFENG BIOLOGICAL SCI & TECH DEV LIMITED
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these antifungal agents only inhibit mold and do not decompose or promote the decomposition of mycotoxins
Soil disinfectant only kills some molds and cannot degrade the toxins produced by molds

Method used

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  • Ecological restoration microbial inoculum capable of inhibiting mildews and degrading mycotoxin

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Embodiment

[0019] An ecological restoration agent capable of inhibiting mold and degrading mycotoxins, composed of Bacillus subtilis ( Bacillus subtilis ACCC10619), Rhodopseudomonas palustris ( Rhodopseudomonas palustris ACCC10649 ), Enterococcus faecalis ( Enterococcus faecalis ACCC10180), Flavobacterium ( Wautersiell falsenii, ACCC19729 ) and Candida utilis ( Candida utilis ACCC20060) mixed composition, in which Bacillus subtilis, Rhodopseudomonas palustris, Enterococcus faecalis and Candida utilis were purchased from China Agricultural Microorganism Culture Collection Center (ACCC), which is also allowed to be used in microbial feed additives by the Ministry of Agriculture Flavobacterium is a strain newly isolated from ginseng planting soil in Tonghua City, Jilin Province. It is identified as Flavobacterium by ACCC gene ( Wautersiellfalsenii) , has been sent to the China Agricultural Microorganism Culture Collection Center (ACCC) for preservation.

[0020] The preparation...

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Abstract

The invention discloses an ecological restoration microbial inoculum capable of inhibiting mildews and degrading mycotoxin. The ecological restoration microbial inoculum consists of bacillus subtilis, rhodopseudomonas palustris, enterococcus faecalis, wautersiellfalsenii and candida utilis which are mixed together. A preparation method of the ecological restoration microbial inoculum comprises the following steps: inoculating strains to corresponding sterilized culture medium for fermentation; performing three-stage amplification culture; and finally, adsorbing and solidifying on straw charcoal to prepare a solid microbial inoculum. The ecological restoration microbial inoculum disclosed by the invention has the advantages of being high in density and activity and excellent in characteristic. The bacillus subtilis, the rhodopseudomonas palustris, the enterococcus faecalis and the newly separated wautersiellfalsenii have an effect of inhibiting mildews and degrading mycotoxin, and particularly by cooperation with the candida utilis, the effect of degrading mycotoxin is obviously increased. The ecological restoration microbial inoculum can be used as a feed additive to solve the problem of mildew and mycotoxin pollution in the feed industry, and can also be used as a soil ecological restoration agent to solve the problem of the spreading of a continuous cropping disease in plantation of ginseng and notoginseng.

Description

technical field [0001] The invention belongs to microbial fermentation technology, in particular to an ecological restoration bacterial agent capable of inhibiting mold and degrading mycotoxins. Background technique [0002] Feed mold contamination is an important factor that directly affects the quality of aquaculture products. According to the estimates of the Food and Agriculture Organization of the United Nations (FAO), at least 25% of the world's grains are currently contaminated by molds and mycotoxins, causing economic losses of up to hundreds of millions of dollars every year. The pollution situation of mold and mycotoxins in my country is even less optimistic. In the Beijing area alone, the mycotoxin pollution rate of grains has reached 90%, of which 15-25% are seriously polluted and should be banned. Mold pollution is more serious in the warmer southern regions. Molds generally produce mycotoxins. For example, common Aspergillus flavus can produce aflatoxins, wh...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K1/16A01N63/00A01N63/04A01P1/00A01P3/00B09C1/10C12R1/125C12R1/01C12R1/46C12R1/72
CPCC12N1/16C12N1/20Y02A40/22Y02P60/21
Inventor 张清敏王希年唐景春郭公宝张海荣徐艳龙郭建斌
Owner JILIN JIUFENG BIOLOGICAL SCI & TECH DEV LIMITED
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