48 results about "Mycotoxin contamination" patented technology

The invention discloses a mycotoxin detoxification agent for feed and a preparation method and a feed additive thereof, and belongs to the field of feed additives. The mycotoxin detoxification agent can be used for detoxification of feed raw materials and compound feed of mycotoxin polluted corn, grains and the like. The mycotoxin detoxification agent consists of modified montmorillonite, yeast cell wall, chitosan and the like; and the preparation method has simple process. The novel mycotoxin detoxification agent for feed can effectively remove pollution of multiple mycotoxins such as aflatoxin, zearalenone, T2 toxin, vomitoxin and the like in the feed raw materials and the feed of the corn, the grains and the like, and prevent various diseases of livestock and poultry because of eating the mycotoxin polluted feed. The mycotoxin detoxification agent has simple preparation process, stable and controllable quality, convenience in use and low price, meanwhile has the advantages of supplementing nutrients, improving the disease resistance of cultured animal bodies and avoiding adsorbing nutrient elements in the feed, and guarantees the health and safety of the cultured animals.

The invention discloses a mold removal agent for feed and a preparation method and a feed additive thereof, belonging to the field of feed additives. The mold removal agent contains modified montmorillonite, yeast cell walls, chitosan, citric acid and Chinese herbal medicinal ingredients, wherein the Chinese herbal medicinal ingredients are prepared from poria cocos, rhizoma alismatis, grifola, cinnamomum cassia, rhizoma atractylodis macrocephalae and rhizoma imperatae. The mold removal agent can be used for removing mold for feed raw materials such as corn and grains and compound feed polluted by mycotoxin so as to prevent livestock and poultry from having various diseases as the livestock and poultry eat the feed polluted by the mycotoxin. The mold removal agent can reduce the absorption of the mycotoxin in the bodies of animals and meanwhile enhances the detoxification ability and the self regulating function of the bodies of animals, achieves the purpose of treating both symptoms and root causes. The mold removal agent has the characteristics of significant curative effect, safety in use, specific effect, stable preparation, controllable quality and simple preparation process, is suitable for industrial production and is convenient to feed.

The invention discloses a method for biodegrading mycotoxin in feed by using a mycotoxin degrading enzyme, and belongs to the field of feed resources. The method comprises the following steps: directly mixing degrading complex enzyme of aflatoxin, zearalenone and deoxynivalenol with moldy feed raw materials, concentrated feed or complete feed; and detoxifying the feed polluted by the mycotoxin to ensure that the degradation rate of the mycotoxin reaches over 80 percent. The method can effectively remove the mycotoxin from the feed, improves animal health and animal production performance, and can effectively solve the problem of feed resource shortage in China.

A method for upgrading human food and animal feed, rendering harmless the contaminating mycotoxins, increasing protein and nucleotide content, providing immuno-modulation, enhancing flavor and palatability is proposed. The method comprises a food functional additive/supplement and feed additive based on yeast biomass processed to enhance the bioavailability and biological activity of its components using dry micron milling with optional agglomeration. The resulting product contains all biologically active components originally present in intact live yeast. Compared to existing practice, the new process is much faster, cheaper, less hardware demanding, less prone to microbial contamination, provides intact biopolymers and results in insoluble product fraction with high surface area. Human food and animal feed containing such additive improves weight gains, feed efficiency, resilience to mycotoxin contamination, improves immunological status, controls intestinal Salmonella and other bacteria and decreases mortality, especially at the young age, replacing antibiotics and growth promoters.

A method of rendering harmless mycotoxins contaminating food, animal feed and assisting infection of plant hosts by microbial parasites is proposed. The method comprises binding mycotoxins by a novel adsorbent, consisting partially or in full of the biomass of filamentous fungi or isolated fungal biomass components, such as chitin, chitozan and hyrdophobins, or fungal biomass enriched in its capacity to bind said mycotoxins using fungal species and strain selection, genetic engineering of fungi, modification of fungal fermentation conditions and downstream physical and chemical treatment of fungal biomass, such as milling or micronization in a dry state. The resulting adsorbent can bind a wide range of mycotoxins, including the ones difficult to bind (Ochratoxin, T-2, DON, NIV) using a current generation of mycotoxin adsorbents based on clay, resins, yeast and bacterial biomass. The adsorbent can be used as an animal feed additive, functional food additive and agricultural fungistatic/fungicide.

The invention provides a feed mold removal agent, comprising the following raw materials in parts by weight: 30-60 parts of organic acid montmorillonite, 20-40 parts of yeast cell walls, 15-25 parts of astragalus polysaccharide, 10-30 parts of pachyman, 5-15 parts of quercetin, 5-15 parts of garlicin, 15-30 parts of bacillus, 15-30 parts of bacterium lacticum, 10-20 parts of humic acid, 20-40 parts of hydrogel polymer, 10-20 parts of fructus kochiae, 10-20 parts of rosin, and 5-15 parts of Chinese mugwort leaf. The feed mold removal agent provided by the invention has no side effect on animals, is efficient in mold removal, prevents livestock and poultry from various diseases caused by eating feeds polluted by mycotoxins, effectively reduces damages caused by the mycotoxins to immune systems of animals, strengthens the immunity of the animals to eat feeds containing the mycotoxins, promotes the growth and development of the livestock and poultry, enhances functions of the immune systems, improves disease resistance of the animals, reduces antibiotic residues in poultry products including meat, eggs and the like, and improves the food safety.

The invention discloses an ecological restoration microbial inoculum capable of inhibiting mildews and degrading mycotoxin. The ecological restoration microbial inoculum consists of bacillus subtilis, rhodopseudomonas palustris, enterococcus faecalis, wautersiellfalsenii and candida utilis which are mixed together. A preparation method of the ecological restoration microbial inoculum comprises the following steps: inoculating strains to corresponding sterilized culture medium for fermentation; performing three-stage amplification culture; and finally, adsorbing and solidifying on straw charcoal to prepare a solid microbial inoculum. The ecological restoration microbial inoculum disclosed by the invention has the advantages of being high in density and activity and excellent in characteristic. The bacillus subtilis, the rhodopseudomonas palustris, the enterococcus faecalis and the newly separated wautersiellfalsenii have an effect of inhibiting mildews and degrading mycotoxin, and particularly by cooperation with the candida utilis, the effect of degrading mycotoxin is obviously increased. The ecological restoration microbial inoculum can be used as a feed additive to solve the problem of mildew and mycotoxin pollution in the feed industry, and can also be used as a soil ecological restoration agent to solve the problem of the spreading of a continuous cropping disease in plantation of ginseng and notoginseng.

A method for the removal of mycotoxins from cereal or oil seed meal that includes the use of a mycotoxin sequestrant to form a food grade composition for human consumption wherein said composition contains no more or less than an FDA approved level of mycotoxin for a human food product.

The invention discloses a mycotoxin adsorbent. The mycotoxin adsorbent comprises the following components in parts by weight: 30-50 parts of bentonite, 1-5 parts of activated carbon, 30-40 parts of an additive A and 5-39 parts of an additive B, wherein the additive A is a yeast cell wall, the additive B is licorice roots or plant extracts, and the plant extracts are radix astragali polysaccharide or ginseng polysaccharide. The invention further discloses a preparation method of the mycotoxin adsorbent. The mycotoxin adsorbent disclosed by the invention has high-efficient adsorption effects on aflatoxins and zearalenone, and is suitable for short-term addition use to raw materials with serious mycotoxin pollution and breeding sites.

The invention discloses a feed additive containing compound algae. The product contains brown alga saccharides, green alga saccharides as well as active substances such as yeast cell walls and chitosan. After compounding, the feed additive can effectively remove pollution of multiple mycotoxins such as aflatoxin, zearalenone, T2 toxins, vomitoxin and fumonisin in feed raw materials such as corns and grains as well as feed, thereby preventing various diseases caused by the fact that livestock and poultry eat the mycotoxin polluted feed, ensuring the animal health and improving the production benefit. The product has the advantages that (1) the harm of the mycotoxins can be comprehensively prevented; (2) all components have synergistic effects and strong detoxication effects, the desorption is difficult after detoxication, and toxins can be more thoroughly removed; (3) the detoxication efficiency is high, and the dosage is less; (4) the feed additive has strong specificity, only acts on the mycotoxins, does not adsorb nutrient elements and does not affect the normal growth of the livestock and poultry; and (5) the immune system can be enhanced, the immune functions of the livestock and poultry can be improved, and the disease resistance of the body can be enhanced.

The invention discloses a method for preparing a mycotoxin adsorbent by using semicoke. The method comprises the steps of uniformly mixing purified semicoke, a bacteriostatic agent, an organic modifier and a grinding aid, then adding the mixture into a ball mill, and executing full grinding, discharging, drying and crushing to obtain a semicoke type bacteriostatic and mildew-removing agent. The mycotoxin adsorbent prepared by the method has the good removal effect on mycotoxin in feed, especially zearalenone and meanwhile can inhibit mold growth, prevent feed mildewing and prolong the shelf life of feed products, and the field of high-value utilization of semi-coke resources is expanded while the problem of mycotoxin pollution is solved.

The invention provides a feed additive for improving egg laying performance of laying hens. The feed additive is prepared from the following raw materials in parts by mass: 0.05-0.1 part of a compoundmicroelement, 1-2 parts of alpha-ketoglutarate, 3-6 parts of daidzein, 3-5 parts of cinnamaldehyde, 10-20 parts of astragalus polysaccharide, 3-5 parts of gamma-aminobutyrate, 12-18 parts of glucoseoxidase, 10-20 parts of living bacteria and 20-30 parts of calcium hydrophosphate; the compound microelement contains zinc methionine, selenium yeast, ferrous lactate, chromium picolinate, manganese sulfate and calcium iodate, and the living bacteria containbacillus subtilis, lactobacillus plantarum, bacillus licheniformis and bacillus coagulans. The feed additive has a remarkable use effect and the high utilization rate, meanwhile the problem that feed is polluted by mycotoxins can be solved, finally the body endocrine balance is maintained, the balance of intestinal flora is adjusted, the absorption of nutrients by the body is promoted, the immunity of the body is enhanced, thus the egg laying rate of the laying hens is increased, the egg quality is improved, and the death and culling rate of the laying hens is decreased.

The present invention discloses a mycotoxin resistant feed additive for pigs and a preparation method and an application thereof. The present invention firstly discloses the mycotoxin resistant feed additive for the pigs. The feed additive comprises lactobacillus rhamnosus, calcined shells and cornstalk charcoal. The preparation method further increases fructus gardeniae and moutan barks on the basis of the mycotoxin resistant feed additive for the pigs, and can significantly improve the removal rate of the mycotoxin, so that the adsorption rates of zearalenone, aflatoxin B1, vomitoxin, etc. can all reach 94% or more, the desorption rates of the toxins are significantly reduced, and the toxin removing is more complete. The raw materials of the lactobacillus rhamnosus, calcined shells, cornstalk charcoal, fructus gardeniae and moutan barks are tested to have obvious synergic effects. The mycotoxin resistant feed additive for the pigs can be used for detoxification of cereals or compound feeds, etc. contaminated by the mycotoxin, prevents the toxic effects of the mycotoxin on the pigs and improves the feed return rate.

The invention discloses bacillus velezensis capable of producing high-yield compound enzyme and efficiently degrading mycotoxin and application of the bacillus velezensis. The bacillus velezensis is bacillus velezensis LB-Y-1, and the bacillus velezensis LB-Y-1 not only can effectively degrade AFB1, but also has the capability of producing protease, cellulase and amylase at high yield. The broiler chicken in-vivo test shows that the strain has the potential probiotic characteristics of high safety, strong stress resistance, easiness in enteral colonization, improvement of intestinal flora structure, improvement of growth performance and the like. In addition, the strain can degrade AFB1 in mildewed peanut meal through fermentation treatment, the content of acid soluble protein is increased, and biological detoxification and quality improvement of the peanut meal are achieved. The comprehensive effects of eliminating and inhibiting mycotoxin pollution in the feed and the raw materials, reducing the crude fiber content of the raw materials, increasing the small peptide content and the like are achieved.

A method and apparatus for detecting the presence of mycotoxins in cereals, the method including: capturing at least one diffuse-light absorption spectrum of a collection of cereal grains; capturing at least one diffuse-light absorption spectrum of at least one individual cereal grain from the collection of cereal grains; and classifying the level of mycotoxin contamination in at least one cereal grain by performing multivariate data analysis on the at least one diffuse-light absorption spectrum of the collection of cereal grains and the at least one diffuse-light absorption spectrum of the at least one individual cereal grain.

The invention discloses mycotoxin-resistant feed additive for pigs, as well as a preparation method and application of the mycotoxin-resistant feed additive. The mycotoxin-resistant feed additive for pigs, firstly disclosed by the invention, comprises lactobacillus rhamnosus, lactobacillus acidophilus and rhodop seudanonas palustris. According to the mycotoxin-resistant feed additive as well as the preparation method and application thereof disclosed by the invention, the dosage of the lactobacillus rhamnosus, the dosage of the lactobacillus acidophilus and the dosage of the rhodop seudanonas palustris are further optimized, the mycotoxin removing effect of the additive prepared from the screened lactobacillus rhamnosus, the screened lactobacillus acidophilus and the screened rhodop seudanonas palustris according to a specific proportion is obviously improved, the adsorption rate of the additive for zearalenone, the adsorption rate of the additive for aflatoxin B1 and the adsorption rate of the additive for vomitoxins respectively reach 95% or above, besides, the toxin desorption rate is notably reduced, and the detoxification is thorough. The mycotoxin-resistant feed additive for pigs, disclosed by the invention, can be used for detoxifying cereals, or compound feeds and the like polluted by mycotoxin, so that the situation that livestock and poultry suffer from various diseases due to eating feeds polluted by the mycotoxin can be avoided, and the feed conversion is increased.

The invention discloses a preparation method of an esterified glucomannan/activated carbon/attapulgite composite mycotoxin adsorbent. The preparation method comprises the following steps: taking sodium phosphates as an attapulgite dispersing agent and a glucomannan esterifying agent, and organically combining attapulgite dispersing, activated carbon material supporting and glucomannan esterifyingby virtue of an integrating technology, thereby obtaining the mycotoxin adsorbent with low cost and high-efficiency detoxification. The product has the effects of effectively preventing mycotoxin pollution in feed and protecting animal health.

The present invention relates to a method for the detection of mycotoxins and their phase I and phase II metabolites in broiler chickens and pigs, the method comprising:

• • collecting the blood of broiler chickens and pigs as a dried blood sample;
  • preparing the dried blood sample for analysis;
  • analyzing the prepared dried blood sample by liquid chromatography-tandem mass spectrometry and high-resolution mass spectrometry.

Such method can advantageously be used for the assessment of the exposure of pigs or broiler chickens to feed contaminated with mycotoxins. Also, such method can be used for assessing the impact of the addition of mycotoxin detoxifying agents to animal feed.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecium GXSCU1 capable of efficiently degrading vomitoxin, a microbial agent and a preparation method and application of the enterococcus faecium GXSCU1. The collection number of the enterococcus faecium GXSCU1 disclosed by the invention is GDMCC (China General Microbiological Culture Collection Center) No.62041. The enterococcus faecium GXSCU1 capable of efficiently degrading the vomitoxin is separated and identified, the degradation rate of the probiotics to the vomitoxin can reach 43.58%-100%, and the harm of the vomitoxin can be effectively eliminated. Besides, the enterococcus faecium GXSCU1 provided by the invention can metabolize vomitoxin into various low-toxicity, non-toxic and even beneficial products such as gamma-sitosterol, theobromine, 3, 7-epoxy caryophyllatin-6-ketone, geraniol, palmitic acid and the like, and plays an important role in reducing mycotoxin pollution and harm of food, agricultural and sideline products, feed and the like.

The present invention provides a fish feed additive preparation method. Montmorillonite and humic acid are added to yeast fermentation broth and bacillus fermentation broth, the pH value of the fermentation broth is adjusted to be 8.5-9, then the fermentation broth is oscillated for 10-15 h, air-dried, and smashed to obtain a complex body, and then the complex body is mixed with other raw materials evenly to obtain the fish feed additives. The fish feed additives obtained by this method can effectively absorb and decompose various types of mycotoxins of the feed raw materials, and solve the problems of serious mycotoxin contamination of domestic feeds. By using the prepared fish feed additives, the mycotoxin removing effect of the feed is ensured, and also the nutrient balance of the feed is improved, and the utilization of the feed is not affected.

The present invention provides a pig feed preparation method. Montmorillonite and humic acid are added to yeast fermentation broth and bacillus fermentation broth, the pH value of the fermentation broth is adjusted to be 8.5-9, then the fermentation broth is oscillated for 10-15 h, air-dried, and smashed to obtain a complex body, and then the complex body is mixed with feed raw materials, puffed, and pelletized to obtain the pig feed. The method can conduct effective absorption and decomposition of various types of mycotoxins of the feed raw materials during the feed preparation process, and solve the problems of serious mycotoxin contamination of domestic feeds. By conducting strict and rational material selection and matching, the mycotoxin removing effect during the preparation process is ensured, and also the nutrient balance of the feed is ensured, and the utilization of the feed is not affected.