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Enterococcus faecium GXSCU1 capable of efficiently degrading vomitoxin, microbial agent as well as preparation method and application of microbial agent

A technology of microbial agent and deoxynivalenol, which is applied in the field of microbiology, can solve the problem that Enterococcus faecium degrades deoxynivalenol and other problems that have not been found, and achieve the effect of eliminating harm

Active Publication Date: 2022-05-06
广西优比特生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the strains that have been found to degrade vomitoxin mainly include Bacillus subtilis, Bacillus cereus, Saccharomyces cerevisiae and Aspergillus oryzae, etc. There is no report on the use of Enterococcus faecium to degrade vomitoxin

Method used

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  • Enterococcus faecium GXSCU1 capable of efficiently degrading vomitoxin, microbial agent as well as preparation method and application of microbial agent
  • Enterococcus faecium GXSCU1 capable of efficiently degrading vomitoxin, microbial agent as well as preparation method and application of microbial agent
  • Enterococcus faecium GXSCU1 capable of efficiently degrading vomitoxin, microbial agent as well as preparation method and application of microbial agent

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preparation example Construction

[0044] The present invention also provides a preparation method of the above-mentioned microbial inoculum, inoculating the Enterococcus faecium GXSCU1 into a culture medium and culturing to obtain the microbial inoculum.

[0045] Unless otherwise specified, the present invention has no special requirements on the source of each component in the medium, and commercially available products well known to those skilled in the art can be used.

[0046] The culture medium of the present invention includes 0.5-4wt.% of glucose, preferably 1-3wt.%, more preferably 2wt.%.

[0047] The culture medium of the present invention includes 0.5-3 wt.% of peptone, preferably 0.8-1.5 wt.%, more preferably 1 wt.%.

[0048] The culture medium of the present invention includes 0.1-2wt.% of yeast powder, preferably 0.3-1wt.%, more preferably 0.5wt.%.

[0049] The culture medium of the present invention includes 0.5-3wt.% of sodium chloride, preferably 0.8-1.5wt.%, more preferably 1wt.%.

[0050] T...

Embodiment 1

[0062] The strain was isolated from a milk sample from a dairy farm in Guangxi. Draw 100 μL of milk sample in the ultra-clean workbench, add it to 900 μL of sterile water for dilution, and dilute to 10 -4 、10 -5 That's it. Then draw 100μL on the plate (media formula: 2% glucose, 1% peptone, 0.5% yeast powder, 1% sodium chloride, 0.2% potassium dihydrogen phosphate, 0.1% dipotassium hydrogen phosphate, 3% agar and 92.2% water) for coating, and after the culture medium was absorbed, the plate was inverted, and placed in an incubator at a culture temperature of 35°C for 24 hours. After 24 hours, pick round, small, raised, smooth, and white colonies for streak culture, and then pick round, small, raised, smooth, white single colonies Save for backup.

[0063] 1. Identification of colony morphology

[0064] The screened GXSCU1 bacterial strain was inoculated on the petri dish by streaking on the plate (the medium on the petri dish was composed of the following components in ma...

Embodiment 2

[0076] A microbial inoculum for degrading vomitoxin, the preparation method of the microbial inoculum is as follows:

[0077] 1) The GXSCU1 bacterial strain screened in Example 1 is activated by streaking on a plate, and its activation medium is composed of the following components in mass percentage: 2% glucose, 1% peptone, 0.5% yeast powder, 1% chlorinated Sodium, 0.2% potassium dihydrogen phosphate, 0.1% dipotassium hydrogen phosphate, 3% agar and 92.2% water; the pH value of the activation medium is 6.0, the temperature of the activation culture is 34-36°C, and the time of the activation culture is 24h ;

[0078] 2) Propagation and large-scale production of bacterial strains: its propagation medium is composed of the following components in mass percentage: 2% glucose, 1% peptone, 0.5% yeast powder, 1% sodium chloride, 0.2% diphosphate Potassium hydrogen phosphate, 0.1% dipotassium hydrogen phosphate and 95.2% water; the pH value of the propagation medium is 6.0, and the ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to enterococcus faecium GXSCU1 capable of efficiently degrading vomitoxin, a microbial agent and a preparation method and application of the enterococcus faecium GXSCU1. The collection number of the enterococcus faecium GXSCU1 disclosed by the invention is GDMCC (China General Microbiological Culture Collection Center) No.62041. The enterococcus faecium GXSCU1 capable of efficiently degrading the vomitoxin is separated and identified, the degradation rate of the probiotics to the vomitoxin can reach 43.58%-100%, and the harm of the vomitoxin can be effectively eliminated. Besides, the enterococcus faecium GXSCU1 provided by the invention can metabolize vomitoxin into various low-toxicity, non-toxic and even beneficial products such as gamma-sitosterol, theobromine, 3, 7-epoxy caryophyllatin-6-ketone, geraniol, palmitic acid and the like, and plays an important role in reducing mycotoxin pollution and harm of food, agricultural and sideline products, feed and the like.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Enterococcus faecium GXSCU1 that efficiently degrades vomitoxin, a microbial agent, a preparation method and application thereof. Background technique [0002] Vomitoxin (VT), also known as deoxynivalenol (DON), belongs to the trichothecene family of compounds, mainly composed of Fusarium graminearum, Fusarium oxysporum, Fusarium moniliforme, Cladosporium Produced by Fusarium fungi such as Fusarium, Pink Fusarium, and Snow Rot Fusarium. There are more than 150 kinds of trichothecene toxins, which are a class of powerful immunosuppressants. The typical symptom caused by them is reduced feed intake, so this type of toxin is also called feed refusal toxin. Deoxynivalenol is one of the harmful toxins with the highest pollution rate in the fields of food, agriculture, and feed. It is called vomitoxin because it can cause vomiting in pigs. [0003] DON exists widely in t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/744A61P39/02A61P1/08A23L5/20C12R1/01
CPCC12N1/20A61K35/744A61P39/02A61P1/08A23L5/28
Inventor 蔡杏华黄凤蝶曹艳红陈学文罗莎莎易萍秦瑞祥陆书发张晓露
Owner 广西优比特生物科技有限公司
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