32 results about "Rhodospirillum rubrum" patented technology

The invention relates to compound microbial fertilizer, which is prepared from a fermented mushroom residue, humic acid, well rotten chicken manure and a compound microbial agent. The compound microbial agent is prepared from rhodospirillum rubrum and 3 to 6 kinds of bacillus megaterium, bacillus subtilis, bacillus licheniformis, bacillus cereus, bacillus latersprorus and rhodopseudomonas palustris. The compound microbial fertilizer provided by the invention can be used for promoting the photosynthesis of a plant and optimizing the composition of nutrient substances in a product; a microbe can be used for dissolving phosphorus and dissolving potassium, improving the fertility of soil, improving the microbial activity of the soil, promoting the nutrient accumulation of a crop and ameliorating product quality. The fermented mushroom residue, the humic acid and the well rotten chicken manure are added, and not only can be used for providing fertilizer for the growth of the microbe, but also can be used for ameliorating the physical and chemical properties of the soil, promoting the formation of the granular structure of the soil, enhancing the air-permeation, water-holding and fertility-accumulation capacity of the soil, improving the utilization rate of the fertilizer and protecting an environment.

Belonging to the field of microbial environmental protection, the invention provides a microbial agent for treatment of sewage and sludge. The microbial agent mainly includes Rhodospirillum rubrum, rhodopseudomonas palustris, bacillus subtilis, Streptomyces microflavus, streptococcus thermophilus, candida utilis, Streptomyces viridis, pseudomonas aeruginosa, virgibacillus halodenitrificans, and Paracoccus denitrificans 10 bacteria. Each bacterium is subjected to conventional slant culture, shaking culture and seed tank cultivation respectively, the bacteria in the seed tank are mixed and then put into sewage directly, symbiosis of flora is utilized to reach the purpose of treating sewage and reducing sludge generation. The microbial agent provided by the invention can be applied to large-scale production and direct adding, is low in cost, and can adapt to changeful sewage components. And the flora has good impact resistance to sewage.

The invention involves a kind to be good lowly, using the sea water cultivation effect bears the salty rapana thomasiana microbial inoculum using the microbial inoculum raise expense the application raise technology. Bears the salty red spirillum the selective breeding for to concentrate, the plate separation, the strain function selective breeding from the sea water, bears the salty red spirillum function strain. Using raise technology including culture medium optimization and application raise craft condition improvement. The culture medium optimization is from carbon source, nitrogen source, ingredient and so on inorganic salt and growth factor carries on the optimization, obtains reduces 47% culture medium ingredient the application formula. Uses the application formula postpositioned using the microbial inoculum raise, the vaccination under the white plastic warm awning the sunlight raise. The invention bears the salty rapana thomasiana strain to be possible to grow under the 0.1~~4%NaCl density, in the fresh water, the sea water cultivation may receive the good application effect. It greatly reduced the photosynthesis bacilliculture cost and the equipment using the raise technology invests. Bears the salty rapana thomasiana microbial inoculum the application raise to issue each milliliter hundred million cells in the above condition the use density to request.

The invention discloses a compound biological synergist for petrochemical wastewater treatment and a preparation method thereof. The compound biological synergist comprises a complex flora and nutrients, wherein the complex flora is Rhodopseudomonas, Rhodospirillum rubrum, Lactobacillus acidophilus, Pediococcus, Bacillus subtilis, yeast, Acinetobacter, Lampropedia sp. and Thiobacillus denitrificans, and the nutrients are sodium humate, glucose, citric acid and glutamic acid coagulate. The invention relates to the technical field of water treatment. By using the compound biological synergist for petrochemical wastewater treatment and the preparation method thereof, the problems that petrochemical wastewater is susceptible to impact and microorganism poisoning is well solved, By adding high-efficiency bacteria and nutrients, the invention enhances the impact resistance and toxicity resistance of the biochemical system, improves the effluent quality, can improve the load for treating organic pollutants and the stability of microbial structure, improves the ability of the biochemical system to resist toxic and harmful substances, and provides reliable guarantee for further improving the effluent quality.

The invention provides a composite microbial agent capable of reducing a COD value of heavy-polluted wastewater. The composite microbial agent is characterized by comprising the following ingredients in parts by weight: 1-8.5 parts of Pseudomonas putida, 2-8 parts of Rhodococcus globerulus, 2-5 parts of Rhodospirillum rubrum, 5-10 parts of Clostridium beijerinckii, 1-5 parts of flocculating candida, 2-5 parts of Acinetobacter johnsonii, 5-12 parts of Acetobacter pasteurianus, 13-34.5 parts of hybrid bacillus and 22.5-48 parts of hybrid Lactobacilli. According to the composite microbial agent provided by the invention, the degradation efficacy and impact resistance to a degraded substrate are strengthened by using synergism of a variety of strain ingredient populations; and a degradation method is high in pertinency, high in response speed and simple in operation.

The invention provides a microbial agent and a preparation method thereof, and relates to the technical field of biology. The present invention provides a microbial agent and a preparation method thereof. The microbial agent is prepared from the following raw materials in parts by weight: 65-90 parts of lactobacillus casei bacterial liquid, 35-45 parts of rhodospirillum rubrum bacterial liquid, 35-45 parts of bacillus subtilis bacterial liquid, 5-9 parts of red algae powder, 10-15 parts of glucose, 8-12 parts of skimmed milk, 6-9 parts of serum, 4-6 parts of guar gum, 7-10 parts of starch, 6-7 parts of sodium bicarbonate, 4-5 parts of citric acid, 4-8 parts of vermiculite powder and 1-2 parts of diatom element. The lactobacillus casei is matched with rhodospirillum rubrum and bacillus subtilis to prepare the microbial agent, the digestive ability of aquatic animals can be improved, harmful germs can be killed, the water environment can be purified, the adaptability of the microbial agent to high-altitude areas is improved by screening low-pressure-resistant flora in a low-pressure environment and then propagating the low-pressure-resistant flora, and the microbial agent is worthy of being vigorously popularized.

The invention discloses a compound microbial inoculum which comprises the following components in parts by weight: 80 to 200 parts of leuconostoc mesenteroides and 40 to 100 parts of rhodospirillum rubrum. Simultaneously, the invention also discloses a preparation method of the microbial inoculum. According to the preparation method, two types of microbial bacteria are used as raw materials, convenience is brought to enterprises to accurately distinguish the raw materials in the process of industrialization, and the cost is greatly reduced. Because microbes have a short metabolism cycle, and cause no pollution to the surrounding environment at all, the microbes are suitable for environmental protection, cultivation and plantation industries.

The invention provides a composite microbial inoculum and a preparation method and application thereof. The preparation method of the composite microbial inoculum comprises the steps of performing independent strain activation on strains with an activation culture medium; and mixing activated strains, to obtain mixed seed liquid, and performing fermentation culture with a fermentation culture medium to obtain the composite microbial inoculum, wherein the strains comprise bacillus pumilus, flavobacterium breve, nitrosomonas, rhodospirillum rubrum and nocardin rhodnii. The composite microbial inoculum is prepared by the preparation method of the composite microbial inoculum. The composite microbial inoculum is used for river pollution abatement. The composite microbial inoculum provided by the application forms a stable microbiological system, and repairing and abating pollution of pollution deposit can be realized.

The invention provides an aquaculture tail water treatment system and treatment method. The treatment system comprises a settling zone, a filtering zone and a filter-feeding animal treatment zone. A composite flora consisting of rhodospirillum rubrum, rhodobacter capsulatus, rhodopseudomonas palustris and rhodotorula mucilaginosa is added into the filter-feeding animal treatment zone. According to the method, the culture tail water is treated by screening the microorganisms with the optimal proportion and combining the microorganisms with the filter-feeding animals. The composite microbial inoculant is combined with filter-feeding animals, and the pilot plant test proves that the effect is good. According to the invention, a composite microecological preparation based on photosynthetic bacteria is developed and combined with filter feeding shellfish to treat aquaculture tail water, so that a very good effect is achieved.

The invention belongs to the technical field of artificial breeding of coral, and particularly relates to a preparation method of composite liquid bait for artificial breeding of coral. Specifically, the composite liquid bait is prepared through the following steps of by using shrimp meat, mussel sexual gland and fish oil as raw materials, adding compound protein enzymes, and performing enzymolysis to prepare enzymolysis supernate and fish oil mixed liquor for standby application; by using ulva lactuca liquid and nannochloropsis oculata as raw materials, preparing nannnochloropsis gaditana and ulva lactuca mixed liquor for standby application; enabling saccharomyces cerevisiae, candida utilis seed liquid and rhodospirillum rubrum seed liquid to be made into mixed bacterium seed liquid for standby application; adding complex vitamins to all of the liquid for standby application so as to obtain the composite liquid bait for artificial breeding of coral. The composite liquid bait for artificial breeding of coral, prepared by the method disclosed by the invention, is comprehensive in nutrient components and good in balance, is harmless, and has the efficacy of purifying water quality, and the survival rate and the growth rate of the artificially bred coral can be increased.

The invention belongs to the technical field of environmental management, and particularly relates to a diversified microbial system for improving eutrophic water and an application thereof. Accordingto the diversified microbial system provided by the invention, the adaptability of microbial products in different environments can be enhanced, so that the degradation stability of eutrophic water is improved. The problem that the existing microbial water purification technology is poor in adaptability and unstable in effect in different water qualities are solved. The microbial system providedby the invention comprises diversified microbial agents and an additive. The diversified microbial agents comprise bacillus subtilis bacterial liquid, bacillus megaterium bacterial liquid, brevibacillus laterosporus bacterial liquid, lactic acid bacteria liquid, nitration monad bacterial liquid, bacillus licheniformis bacterial liquid, trichoderma viride bacterial liquid, saccharomyces cerevisiaebacterial liquid, pseudomonas fluorescens bacterial liquid, rhodospirillum rubrum bacterial liquid, rhodopseudomonas palustris bacterial liquid, spherical rhodobacter sphaeroides bacterial liquid, andpseudomonas putida bacterial liquid. The additive comprises starch, diatomite, calcium carbonate and compound amino acid.

The invention provides an efficient and digestible biological fermentation feed, and relates to the technical field of feeds. The efficient and digestible biological fermentation feed is prepared from 270 to 290 parts of animal waste, 2 to 3 parts of thermophilic aerobic bacteria liquid, 120 to 130 parts of straw, 90 to 110 parts of wheat bran, 2 to 3 parts of compound bacteria liquid, 1 to 2 parts of lactobacillus casei liquid, 1 to 2 parts of bifidobacterium liquid, 1 to 2 parts of rhodospirillum rubrum liquid, 30 to 35 parts of barley leaf powder, 22 to 26 parts of silkworm chrysalis meal, 10 to 15 parts of calcium malate, 2 to 3 parts of herba leonuri extract, 5 to 8 parts of diatom and 0.01 to 0.02 part of expanded vermiculite powder. Harmful germs and worm eggs are killed through thermophilic aerobic bacteria, animal waste, straw and wheat bran are decomposed through compound bacteria liquid, components easy to absorb and microbial active probiotics are obtained, clostridium butyricum is matched with lactobacillus casei, bifidobacterium, tea polyphenol, barley leaf powder and expanded vermiculite powder to achieve the effect of resisting diseases and insect pests, and the digestive ability of fishes is enhanced.

The invention relates to a method for producing hydrogen by utilizing photosynthetic bacteria. The method comprises the steps of: the strain activation of Rhodospirillum rubrum ATCC11170; the seed culture of the strain; the fermentation culture of the strain in incandescent light and natural daylight in a lighting culture room; the collection of the hydrogen till the production of hydrogen is stopped. Two phases of lighting culture of the invention can obviously improve the photosynthetic hydrogen production rate. The method of the invention is easy to be operated, has low cost and wide application prospect.

The invention provides a photosynthetic bacterium culture medium and a preparation method thereof, each liter of the culture medium comprises the following components: 0.05 g of potassium dihydrogen phosphate, 1.0 g of table salt or crude salt, 0.1 g of monosodium glutamate, 0.02 g of vitamin B complex and 50 ml of alcohol, yellow serofluid is supplemented to 1 L, the pH is adjusted to 6.8-7.6, and the yellow serofluid is hydrolyzed yellow serofluid. The photosynthetic bacteria culture medium contains proper carbon sources, nitrogen sources, trace elements and growth factors, is comprehensive in nutrition, can ensure normal growth requirements of photosynthetic bacteria, is simple in components and preparation method, is easy to obtain and cheap in raw materials, is stable in source, and is suitable for large-scale production. The culture medium is suitable for large-scale commercial activation and propagation culture of various common photosynthetic bacteria strains such as rhodopseudomonas palustris, rhodopseudomonas capsulatus and rhodospirillum rubrum.

The present invention relates to a recombinant host cell, comprising in its genome preferably heterologous gene expression cassettes capable of being expressed in the host cell, the gene expression cassettes encoding a nucleic acid sequence encompassing at least 80% of the full-length sequence of the mamAB operon, the mamGDFC operon, and the tmms6 operon of a magnetotactic alphaproteobacterium, respectively, and preferably further comprising gene expression cassettes capable of being expressed in the host cell, the gene expression cassettes encoding a nucleic acid sequence encompassing at least 80% of the full-length sequence of the mamXY operon and/or the feoABI operon of a magnetotactic alphaproteobacterium; wherein the recombinant host cell, upon expression of the gene expression cassettes in their entirety, is capable of producing magnetic nanoparticles. Preferably, the recombinant host cell is derived from Rhodospirillum rubrum. Furthermore, the present invention also relates to a corresponding method for producing such recombinant host cell by means of genetic transposition.

The invention relates to: a method of treating liquid waste (liquid agricultural or industrial effluents or aquatic sites) which is loaded or polluted with tributyl phosphate (TBP), modified bacterial strains which can be used in the aforementioned treatment method, a method for monitoring changes in TBP pollution, and the device which is used to perform said treatment method. According to the invention, the liquid waste-treatment or -purification method essentially comprises: steps (1) consisting in bringing said liquid waste into contact with at least one non-sulphur purple photosynthetic bacterial strain which is resistant to TBP and which is selected from the group containing Rhodopseudomonas palustris (Rp. palustris), Rhodospirillum rubrum (Rs. rubrum), Rhodobacter capsulatus (Rb. capsulatus) or Rhodobacter sphaeroides (Rb. Sphaeroides) as well as the aforementioned modified bacterial strains in order to overexpress cytochrome P450 in conditions that enable the degradation of the TBP present in said waste, regardless of the initial TBP concentration; and (2) the recovery of the purified liquid effluents.

The invention discloses a method and agent for preventing and treating a cyst nematode disease of beet. According to the method, rhodospirillum rubrum is applied to prevention and treatment of the cyst nematode disease of beet. In specific application, a bacterial suspension with a concentration of 1-2 * 10<8> cfu/ml is prepared from rhodospirillum rubrum and water; beet seedlings are transplanted after two pairs of true leaves of the beet seedlings are grown, root irrigation treatment via the bacterial suspension is conducted on the seedlings one day before transplanting, 1 ml of the bacterial suspension is used for root irrigation of each seedling, and then 20 ml of rhodospirillum rubrum with a concentration of 0.5-1 * 10<7> cfu/ml is applied for each seedling after transplanting. The method and the agent can inhibit and kill beet cyst nematodes, so the yield of beet is increased; and since photosynthetic bacteria are easy to culture, non-toxic and environment-friendly, the method and the agent have the advantages of low cost, high benefit, no environmental pollution, no toxic residue and the like.

A rice-shrimp polyculture field microbial complex inoculant is prepared from four stains including photosynthetic bacterium, bacillus subtilis, zinc sulfate, bacillus licheniformis and sugarbeet saccharose serving as an additive; in parts by mass, 18 parts of photosynthetic bacterium, 12 parts of bacillus subtilis, 20 parts of sugarbeet saccharose, 2 parts of zinc sulfate and 5 parts of bacillus licheniformis are mixed to prepare a mixed agent; the microbial complex inoculant further comprises 43 parts, by mass, of pure water; the pure water serves as a diluent to be mixed with the mixed agentto prepare the complex inoculant; the effective number of living bacteria of the photosynthetic bacterium, the bacillus subtilis and the bacillus licheniformis in the complex inoculant is higher thanor equal to 0.2 billion/liter; the applied photosynthetic bacteria can be rhodopseudomonas palustris; the applied bacillus licheniformis can be bacillus latersprorus and paenibacillus polymyxa; and the photosynthetic bacteria can be rhodospirillum rubrum and purple nonsulfur bacteria.

The invention discloses a novel composite photosynthetic bacteria agent, which is composed of the following ingredients in parts by weight: 1-3 parts of Rhodospirillum rubrum, 2-5 parts of Streptococcus faecalis, 4-6 parts of Bacillus licheniformis, and aerobic spores 2-6 parts of Bacillus, 1-6 parts of Lactobacillus acidophilus, 5-7 parts of Nitrosobacillus, 3-5 parts of Cellulomonas, 4-8 parts of Bacillus natto, 1-9 parts of Kluyveromyces lactis and 3‑6 parts of Bacillus subtilis. The invention is favorable for popularization and application.

The invention discloses a compound photosynthetic microbial agent with long shelf life. The compound photosynthetic microbial agent is prepared from the following components in parts by weight: 1 to 3parts of rhodospirillum rubrum, 2 to 5 parts of rhodopseudomonas palustris, 4 to 6 parts of rhodopseudomonas, 2 to 6 parts of anaerobion, 1 to 3 parts of nitrobacteria, 1 to 6 parts of lactobacillusacidophilus, 5 to 7 parts of nitrosomas, 3 to 5 parts of cellulomonas, 4 to 8 parts of bacillus natto, 1 to 9 parts of lactic acid kluyveromyces and 3 to 6 parts of bacillus subtilis. The compound photosynthetic microbial agent disclosed by the invention is convenient to popularize and apply.