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Compound bacterial agent and its preparation method and application

A technology of composite bacterial agents and strains, applied in the field of river pollution control, can solve problems such as not being able to meet the needs of sediment pollution control

Active Publication Date: 2021-03-23
HUNAN AIRBLUER ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, a biological preparation of bacteria or enzymes obviously cannot meet the needs of sediment pollution control.

Method used

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  • Compound bacterial agent and its preparation method and application
  • Compound bacterial agent and its preparation method and application
  • Compound bacterial agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] (1) Strains

[0094] Take the corresponding strains for later use, and then culture them. For the culture process, see figure 1 , as described below:

[0095] (2) Activation of slant strains

[0096] Activation medium formula: 0.5% soybean peptone, 0.5% yeast extract powder, 1% glucose, 2% agar, 0.3% beef extract, 0.8% potassium dihydrogen phosphate, 0.08% manganese sulfate (the above is the mass fraction), and the balance is distilled water.

[0097] After mixing the raw materials evenly, adjust the pH to 7.1 with 20% NaOH aqueous solution, and sterilize at 121° C. for 30 minutes.

[0098] Streak inoculation of the above-mentioned 5 strains on the respective test tube slant surface activation medium, and culture at 36°C for 19 hours to activate the strains.

[0099] (3) Basic training

[0100] Basal medium formula: 2.0% glucose, 2.5% corn steep liquor, 1.5% bean cake hydrolyzate, 0.3% fish meal, 0.2% potassium dihydrogen phosphate, 0.3% ammonium sulfate, 0.05% mag...

Embodiment 2

[0119] (1) Strains

[0120] Take the corresponding bacteria for use.

[0121] (2) Activation of slant strains

[0122] Activation medium formula: 0.4% soybean peptone, 0.6% yeast extract powder, 0.8% glucose, 2.2% agar, 0.2% beef extract, 1.0% potassium dihydrogen phosphate, 0.06% manganese sulfate (the above is the mass fraction), and the balance is distilled water.

[0123] After mixing the raw materials evenly, adjust the pH to 7.2 with 20% NaOH aqueous solution, and sterilize at 125° C. for 25 minutes.

[0124] Streak inoculation of the above-mentioned 5 strains on the activation medium on the slant of each test tube, and culture at 37°C for 18 hours to activate the strains.

[0125] (3) Basic training

[0126] Basal medium formula: 1.8% glucose, 2.6% corn steep liquor, 1.4% soybean cake hydrolyzate, 0.4% fish meal, 0.1% potassium dihydrogen phosphate, 0.4% ammonium sulfate, 0.04% magnesium sulfate, 0.12% urea (the above is the mass fraction) , and the balance is dist...

Embodiment 3

[0144] (1) Strains

[0145] Take the corresponding bacteria for use.

[0146] (2) Activation of slant strains

[0147] Activation medium formula: 0.6% soybean peptone, 0.4% yeast extract powder, 1.2% glucose, 1.8% agar, 0.4% beef extract, 0.6% potassium dihydrogen phosphate, 0.10% manganese sulfate (the above is the mass fraction), and the balance is distilled water.

[0148] After mixing the raw materials evenly, adjust the pH to 7.0 with 20% NaOH aqueous solution, and sterilize at 120° C. for 35 minutes.

[0149] Streak inoculation of the above five strains on the activation medium on the slant of the test tube, and culture at 35°C for 20 hours to activate the strains.

[0150] (3) Basic training

[0151] Basic medium formula: 1.9% glucose, 2.4% corn steep liquor, 1.6% soybean cake hydrolyzate, 0.2% fish meal, 0.3% potassium dihydrogen phosphate, 0.2% ammonium sulfate, 0.06% magnesium sulfate, 0.08% urea (the above is the mass fraction) , and the balance is distilled wa...

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Abstract

The invention provides a composite bacterial agent and its preparation method and application. The preparation method of the composite bacterial agent: the bacterial strains are independently activated using the activation medium; the activated bacterial strains are mixed to obtain a mixed seed liquid, and then the fermentation medium is used for fermentation and cultivation to obtain the composite bacterial agent; Species include Bacillus pumilus, Flavobacterium brevis, Nitrosomonas, Rhodospirillum rubrum and Nocardia rubrum. The composite bacterial agent is prepared by using the preparation method of the composite bacterial agent. Application of compound bacterial agent for river pollution control. The composite microbial agent provided by the application forms a stable micro-ecological system, realizes the restoration of polluted sediment and eliminates pollution.

Description

technical field [0001] The invention relates to the field of river pollution control, in particular to a composite bacterial agent and its preparation method and application. Background technique [0002] River sediment pollution is a prominent environmental problem at present. Its pollutants mainly enter the water body through sewage discharge, soil erosion, rainwater leaching and scouring, atmospheric deposition, etc., and are finally deposited in the sediment and gradually enriched, making the sediment heavily polluted. The research on sediment pollution remediation has become a hot spot in the field of river pollution control. [0003] At present, the remediation technologies of river channel polluted sediment mainly fall into three categories: physical remediation, chemical remediation and biological remediation. The use of biotechnology and biological science to remediate polluted river sediments has gradually become a hot spot in sediment pollution control. [0004...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/20C12R1/365C12R1/01C02F103/00
CPCC02F3/341C02F2103/007C12N1/20
Inventor 张新兵胡志鑫廖凯强刘书洪
Owner HUNAN AIRBLUER ENVIRONMENTAL PROTECTION TECH
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