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Compound microbial inoculum and preparation method thereof

A technology of composite microbial agent and total bacterial count, which is applied in the field of microorganisms, can solve problems such as difficulty in reducing costs, restricting the development of industrialization, and complicated procedures, and achieve the effects of reducing costs, reducing the number of bacteria used, and shortening the metabolic cycle

Inactive Publication Date: 2011-09-21
JIANGSU BICHENG ENVIRONMENTAL PROTECTION EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the effect of EM is good, there are still some factors that prevent it from being widely promoted in our country.
First of all, in terms of economy, the high cost of introducing bacteria makes most of the benefits handed over to foreign countries, and it is difficult to reduce the cost; secondly, in terms of quality technology, more than 80 kinds of microorganisms are mixed together, on the one hand, it is difficult for enterprises to accurately identify and distinguish. The development of industrialization, on the other hand, the process is extremely complicated, and a little carelessness may lead to the death of effective strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Carry out amplified culture respectively:

[0020] After the Leucobacterium enterica strain was activated on a slant, it was inoculated in a liquid medium and cultured at 28°C for 72 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween 800.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0021] Activate Rhodospirillum rubrum on a slant, inoculate it in a liquid medium, and culture it at 28°C for 72 hours. The liquid medium formula: 0.1% ammonium chloride, 3.5% sodium acetate, 0.01% magnesium chloride, 0.01% calcium chloride, diphosphate Potassium hydrogen 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract 0.01%, pH6.0.

[0022] For counting live bacteria, detect the number of bacteria of Leuconostoma enteritidis and the number of bacteria of Rhodospira rubrum, and mix them according to parts by weight: 80 parts of Leuconostoma enteritidis and 40 parts of R...

Embodiment 2

[0025] (1) Carry out amplified culture respectively:

[0026] After the Leucobacterium enterica strain was activated on a slant, it was inoculated in a liquid medium and cultured at 32°C for 48 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween 800.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0027] Activate Rhodospirillum rubrum on a slant, inoculate it in a liquid medium, and culture it at 32°C for 48 hours. The liquid medium formula: 0.1% ammonium chloride, 3.5% sodium acetate, 0.01% magnesium chloride, 0.01% calcium chloride, diphosphate Potassium hydrogen 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract 0.01%, pH6.0.

[0028] Viable bacteria count, detecting the number of bacteria of Leuconostoma enteritidis and the number of bacteria of Rhodospira rubrum, and mixing them according to parts by weight: 150 parts of Leuconostoma enteritidis and 75 parts of...

Embodiment 3

[0031] (1) Carry out amplified culture respectively:

[0032] After the Leucobacterium enterica strain was activated on a slant, it was inoculated in a liquid medium and cultured at 35°C for 36 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween 800.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0033] Activate Rhodospirillum rubrum on a slant, inoculate it in a liquid medium, and culture it at 35°C for 36 hours. The liquid medium formula: 0.1% ammonium chloride, 3.5% sodium acetate, 0.01% magnesium chloride, 0.01% calcium chloride, diphosphate Potassium hydrogen 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract 0.01%, pH6.0.

[0034] Viable bacteria count, detecting the number of bacteria of Leuconostoma enteritidis and the number of bacteria of Rhodospira rubrum, and mixing them according to parts by weight: 200 parts of Leuconostoma enteritidis and 100 parts o...

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Abstract

The invention discloses a compound microbial inoculum which comprises the following components in parts by weight: 80 to 200 parts of leuconostoc mesenteroides and 40 to 100 parts of rhodospirillum rubrum. Simultaneously, the invention also discloses a preparation method of the microbial inoculum. According to the preparation method, two types of microbial bacteria are used as raw materials, convenience is brought to enterprises to accurately distinguish the raw materials in the process of industrialization, and the cost is greatly reduced. Because microbes have a short metabolism cycle, and cause no pollution to the surrounding environment at all, the microbes are suitable for environmental protection, cultivation and plantation industries.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a composite microbial bacterial agent and a preparation method thereof. Background technique [0002] With the continuous development of biotechnology, microbial agents are widely used in the fields of environmental protection, breeding and planting. In the early 1980s, Professor Teruo Higa of the Ryukyu University in Japan took the lead in inventing a compound microecological preparation EM (Effective Microbial Bacteria) composed of more than 80 kinds of microorganisms belonging to 10 genera, including acetic acid mother bacteria, lactic acid bacteria, photosynthetic bacteria, and actinomycetes. group). The promotion and application of EM in Japan, Brazil, the United States and other places has achieved great success. [0003] Although the effect of EM is good, there are still some factors that prevent it from being widely promoted in our country. First of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12R1/01
Inventor 李南华李维炯李春燕
Owner JIANGSU BICHENG ENVIRONMENTAL PROTECTION EQUIP
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