Microbial agent and preparation method thereof

A microbial inoculum, Rhodospirillum technology, applied in the field of microorganisms, can solve the problems of difficulty in reducing costs, restricting the development of industrialization, complex procedures, etc., and achieves the effects of reducing costs, reducing the number of bacteria used, and shortening the metabolic cycle.

Inactive Publication Date: 2011-09-28
JIANGSU BICHENG ENVIRONMENTAL PROTECTION EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the effect of EM is good, there are still some factors that prevent it from being widely promoted in our country.
First of all, in terms of economy, the high cost of introducing bacteria makes most of the benefits handed over to foreign countries, and it is difficult to reduce the cost; secondly, in terms of quality technology, more than 80 kinds of microorganisms are mixed together, on the one hand, it is difficult for enterprises to accurately identify and distinguish. The development of industrialization, on the other hand, the process is extremely complicated, and a little carelessness may lead to the death of effective strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Carry out amplified culture respectively:

[0020] After the Bacillus pumilus is activated on the slant, it is inoculated in a liquid medium and cultured at 28° C. for 72 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH 6.5.

[0021] Rhodospirillum mesophilus was activated on a slant, inoculated in a liquid medium, and cultured at 28°C for 72 hours. The liquid medium formula: 0.1% ammonium chloride, 3.5% sodium acetate, 0.01% magnesium chloride, 0.01% calcium chloride, diphosphate Potassium hydrogen 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract 0.01%, pH6.0.

[0022] Viable bacteria count, detecting the number of bacteria of Bacillus pumilus and the number of bacteria of Rhodospirillum meshalophilus, and mixing them according to parts by weight: 40 parts of Bacillus pumilus and 20 parts of Rhodospirillum meshalophilus.

[0023] (2) Add 73% of rice bran, 10% of bran, 7% of soybean powder, 8% of milk po...

Embodiment 2

[0025] (1) Carry out amplified culture respectively:

[0026] After the bacillus pumilus is activated on the slant, it is inoculated in a liquid medium and cultured at 32° C. for 48 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH 6.5.

[0027] Rhodospirillum mesophilus was activated on a slope, inoculated in a liquid medium, and cultured at 32°C for 48 hours. The liquid medium formula: 0.1% ammonium chloride, 3.5% sodium acetate, 0.01% magnesium chloride, 0.01% calcium chloride, diphosphate Potassium hydrogen 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract 0.01%, pH6.0.

[0028] Viable bacteria counting, detecting the number of bacteria of Bacillus pumilus and the number of bacteria of Rhodospirillum meshalophilus, and mixing them according to parts by weight: 70 parts of Bacillus pumilus and 35 parts of Rhodospirillum meshalophilus.

[0029] (2) Add 73% of rice bran, 10% of bran, 7% of soybean powder, 8% of milk...

Embodiment 3

[0031] (1) Carry out amplified culture respectively:

[0032] After the bacillus pumilus is activated on the slant, it is inoculated in a liquid medium and cultured at 35° C. for 36 hours. The liquid medium formula is: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH 6.5.

[0033] Rhodospirillum mesophilus was activated on a slant, inoculated in a liquid medium, and cultured at 35°C for 36 hours. The liquid medium formula: 0.1% ammonium chloride, 3.5% sodium acetate, 0.01% magnesium chloride, 0.01% calcium chloride, diphosphate Potassium hydrogen 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract 0.01%, pH6.0.

[0034] Viable bacteria counting, detecting the number of bacteria of Bacillus pumilus and the number of bacteria of Rhodospirillum meshalophilus, and mixing them in parts by weight: 100 parts of Bacillus pumilus and 50 parts of Rhodospirillum meshalophilus.

[0035] (2) Add 73% rice bran, 10% bran, 7% soybean powder, 8% milk powder, and 1% tr...

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Abstract

The invention discloses a microbial agent, comprising, by weight, 40 to 100 parts of Bacillus pumilus and 20 to 50 parts of mesohaline Rhodospirillaceae. The invention simultaneously discloses a preparation method for the microbial agent. In the invention, two microbial bacteria are employed as the raw materials, which is convenient for enterprises to accurately discriminate the two in an industrial production, thereby reducing cost. Since microbes have a short metabolism cycle and produce no pollution to the surroundings, the microbial agent provided in the invention is applicable to the industries of environmental protection, culturing and plantation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial agent and a preparation method thereof. Background technique [0002] With the continuous development of biotechnology, microbial agents are widely used in the fields of environmental protection, breeding and planting. In the early 1980s, Professor Teruo Higa of the Ryukyu University in Japan took the lead in inventing a compound microecological preparation EM (Effective Microbial Bacteria) composed of more than 80 kinds of microorganisms belonging to 10 genera, including acetic acid mother bacteria, lactic acid bacteria, photosynthetic bacteria, and actinomycetes. group). The promotion and application of EM in Japan, Brazil, the United States and other places has achieved great success. [0003] Although the effect of EM is good, there are still some factors that prevent it from being widely promoted in our country. First of all, in terms of eco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12R1/01C12R1/07
Inventor 李南华李维炯李春燕
Owner JIANGSU BICHENG ENVIRONMENTAL PROTECTION EQUIP
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