Aquaculture tail water treatment system and treatment method
An aquaculture and treatment system technology, applied in biological water/sewage treatment, water/sewage multi-stage treatment, water/sludge/sewage treatment, etc., can solve the problems of ecological environment destruction, nitrogen and phosphorus pollutants, etc. good effect
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Embodiment 1
[0025] Example 1: Constructing a marine photosynthetic complex microbial system with optimal ratios
[0026] Six strains of photosynthetic bacteria and one strain of marine yeast were selected through preliminary experiments, namely Pseudomonas putida, Chlorobium limicola, Chromatiumminus, Rhodospirillum rubrum), Rhodobacter capsulatus, Rhodopseudomonas palustris and Rhodotorulamucilaginosa, Rhodospirilla rubrum, Capsulatus capsulatus and Rhodotorula mucilaginosa were screened out through pretreatment experiments Match the ratio of Monascus and Rhodotorula japonicus.
[0027] 1. Single bacteria experiment
[0028] Centrifuge the bacteria sample at 8000RPM for 10 minutes, dilute it with sterilized water sample, adjust to OD600 value 0.5, set the input amount of each bacteria 1‰, 2‰, 3‰, 4‰, 5‰, five concentration gradients , according to the amount of input into 500ml of sewage, each group set up 2 parallel, with the tail water without bacteria solution for comparison.
[00...
Embodiment 2
[0043] Embodiment 2: Preparation of composite flora
[0044] First, activate the bacteria, the specific steps are as follows:
[0045] ①Prepare a test tube containing 5-10mL liquid medium and 2 plates for each type of bacteria, see Table 4 for the preparation method;
[0046] ② Open the freeze-dried tubes containing 5 kinds of bacteria powder in the safety cabinet, burn the neck with an alcohol lamp, then quickly drop sterile water to break it, and then smash it with tweezers;
[0047] ③Pipe 0.5mL of liquid culture medium into the freeze-drying tube, dissolve it fully, put it back into the liquid test tube, and mix well;
[0048] ④Pipe 0.2mL of the bacterial suspension into the plate, spread it evenly, repeat twice to obtain two plates; culture the plate according to the following culture conditions, culture Rhodotorula glucosa according to the medium described in Table 4, and rhodopseudomonas palustris Bacteria, Rhodobacter capsulata and Rhodospirillum rubrum were cultured ...
Embodiment 3
[0054] Example 3: Application of composite bacterial agent to aquaculture tail water treatment under pilot test conditions
[0055] The optimal ratio compound bacterial agent screened out in the laboratory experiment was put into the pilot test to simulate the comprehensive bioremediation system for the experiment, and the water quality indicators were measured in the early stage, middle and late stage of the experiment. figure 2 It is the experimental flow chart of the bioremediation system. The whole system has four treatment steps, namely, the sedimentation area, the brush area (filter area), the filter-feeding shellfish area and the artificial wet area. In the present invention, the compound bacterial agent is added to the filter-feeding shellfish area, and the input amount of the compound bacterial agent is 3‰ as the experimental group, and the system without adding the bacterial liquid is used for comparison.
[0056] image 3 It is a comparison of the water quality tr...
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