Method for producing hydrogen using photosynthetic bacteria

A technology for producing hydrogen and photosynthetic bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Active Publication Date: 2012-01-11
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the effect of light quality on the hydrogen production of photosynthetic bacteria

Method used

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  • Method for producing hydrogen using photosynthetic bacteria
  • Method for producing hydrogen using photosynthetic bacteria
  • Method for producing hydrogen using photosynthetic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1: Use R. rubrum ATCC11170 to prepare hydrogen under the condition of continuous light of incandescent lamp

[0075] Hydrogen gas is produced as follows:

[0076] (1) Strain activation: R. rubrum ATCC11170 was inoculated on SMN plate medium, and cultured at 30°C for 5 days; the obtained activated R. rubrum colonies turned dark red, and the colony diameter reached 2 mm or more and could be used in subsequent steps.

[0077] (2) Seed culture: Inoculate the activated bacterial classification of step (1) in a 50ml Erlenmeyer flask with 30-40ml SMN liquid medium, and inoculate at 30°C and a shaking speed of 100rpm in a shaking incubator produced by Harbin Donglian Company The seed culture solution was obtained by shaking and culturing in the dark for 24 hours; the obtained seed culture solution had a light absorption value at 600 nm greater than 2.5 and could be used in subsequent steps.

[0078] (3) Fermentation culture: using the volume of 25ml anaerobic tubes ...

Embodiment 2

[0083] Example 2: Using R. rubrum ATCC11170 to prepare hydrogen in two-stage light culture with incandescent lamp as light energy source (light filter culture for 48h)

[0084] Hydrogen gas is produced as follows:

[0085] The present embodiment carries out according to the steps (1), (2) and (4) of embodiment 1, and step (3) is carried out according to the following manner:

[0086] Photosynthetic hydrogen evolution under two-stage light culture.

[0087] The first stage is filter culture:

[0088] Firstly, take an anaerobic tube sold under the trade name anaerobic culture tube by the College of Life Sciences of Capital Normal University, fill it with the inoculation culture solution obtained in step (2), and seal the anaerobic tube in a blue glass container. Inside the carton, the top of the carton is covered with tinfoil, and the incandescent lamp provides light energy. The characteristics of the blue glass are as described above. The light intensity on the surface of the...

Embodiment 3

[0092] Example 3: Using R. rubrum ATCC11170 to produce hydrogen by two-stage light culture with incandescent lamp as light energy source

[0093] Hydrogen gas is produced as follows:

[0094] The present embodiment carries out according to the steps (1), (2) and (4) of embodiment 1, and step (3) is carried out according to the following manner:

[0095] Photosynthetic hydrogen evolution under two-stage light culture after different light-filtered culture times.

[0096] The first stage is filter culture:

[0097] First, take an anaerobic tube sold under the trade name anaerobic culture tube by the School of Life Sciences of Capital Normal University, fill it with the inoculation culture solution obtained in step (2), and place the inoculated anaerobic tube in a blue-blue tube. In the glass carton, the top of the carton is covered by tinfoil, and the incandescent lamp provides light energy. The light intensity on the surface of the carton installed with blue glass is 2800lux ...

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Abstract

The invention relates to a method for producing hydrogen by utilizing photosynthetic bacteria. The method comprises the steps of: the strain activation of Rhodospirillum rubrum ATCC11170; the seed culture of the strain; the fermentation culture of the strain in incandescent light and natural daylight in a lighting culture room; the collection of the hydrogen till the production of hydrogen is stopped. Two phases of lighting culture of the invention can obviously improve the photosynthetic hydrogen production rate. The method of the invention is easy to be operated, has low cost and wide application prospect.

Description

【Technical field】 [0001] The invention relates to the field of bioenergy, and more specifically, the invention relates to a method for producing hydrogen by using a photosynthetic bacterium. 【Background technique】 [0002] Energy is an important material basis for human survival and development. Since the beginning of the 21st century, with the accelerated pace of industrialization and urbanization in our country, the demand for energy has experienced an unprecedented high growth trend, and energy and environmental issues have become the focus of more attention. To achieve sustainable development and meet future energy needs, it is necessary to optimize and diversify the energy structure. The accelerated development of renewable and clean energy will help achieve sustainable energy development and strengthen environmental protection. [0003] Hydrogen energy is known as one of the new energy sources with the most potential for development. It has a high combustion calorifi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 朱瑞艳林涛李季伦
Owner 领先生物农业股份有限公司
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