Composite microbial inoculum and preparation method and application thereof

A technology of composite bacterial agents and strains, applied in the field of river pollution control, can solve problems such as not being able to meet the needs of sediment pollution control

Active Publication Date: 2019-11-19
HUNAN AIRBLUER ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, a biological preparation of bacteria or enzyme

Method used

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  • Composite microbial inoculum and preparation method and application thereof
  • Composite microbial inoculum and preparation method and application thereof
  • Composite microbial inoculum and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] (1) Strains

[0094] Take the corresponding strains for later use, and then culture them. For the culture process, see figure 1 , as described below:

[0095] (2) Activation of slant strains

[0096] Activation medium formula: 0.5% soybean peptone, 0.5% yeast extract powder, 1% glucose, 2% agar, 0.3% beef extract, 0.8% potassium dihydrogen phosphate, 0.08% manganese sulfate (the above is the mass fraction), and the balance is distilled water.

[0097] After mixing the raw materials evenly, adjust the pH to 7.1 with 20% NaOH aqueous solution, and sterilize at 121° C. for 30 minutes.

[0098] Streak inoculation of the above-mentioned 5 strains on the respective test tube slant surface activation medium, and culture at 36°C for 19 hours to activate the strains.

[0099] (3) Basic training

[0100] Basal medium formula: 2.0% glucose, 2.5% corn steep liquor, 1.5% bean cake hydrolyzate, 0.3% fish meal, 0.2% potassium dihydrogen phosphate, 0.3% ammonium sulfate, 0.05% mag...

Embodiment 2

[0119] (1) Strains

[0120] Take the corresponding bacteria for use.

[0121] (2) Activation of slant strains

[0122] Activation medium formula: 0.4% soybean peptone, 0.6% yeast extract powder, 0.8% glucose, 2.2% agar, 0.2% beef extract, 1.0% potassium dihydrogen phosphate, 0.06% manganese sulfate (the above is the mass fraction), and the balance is distilled water.

[0123] After mixing the raw materials evenly, adjust the pH to 7.2 with 20% NaOH aqueous solution, and sterilize at 125° C. for 25 minutes.

[0124] Streak inoculation of the above-mentioned 5 strains on the activation medium on the slant of each test tube, and culture at 37°C for 18 hours to activate the strains.

[0125] (3) Basic training

[0126] Basal medium formula: 1.8% glucose, 2.6% corn steep liquor, 1.4% soybean cake hydrolyzate, 0.4% fish meal, 0.1% potassium dihydrogen phosphate, 0.4% ammonium sulfate, 0.04% magnesium sulfate, 0.12% urea (the above is the mass fraction) , and the balance is dist...

Embodiment 3

[0144] (1) Strains

[0145] Take the corresponding bacteria for use.

[0146] (2) Activation of slant strains

[0147] Activation medium formula: 0.6% soybean peptone, 0.4% yeast extract powder, 1.2% glucose, 1.8% agar, 0.4% beef extract, 0.6% potassium dihydrogen phosphate, 0.10% manganese sulfate (the above is the mass fraction), and the balance is distilled water.

[0148] After mixing the raw materials evenly, adjust the pH to 7.0 with 20% NaOH aqueous solution, and sterilize at 120° C. for 35 minutes.

[0149] Streak inoculation of the above five strains on the activation medium on the slant of the test tube, and culture at 35°C for 20 hours to activate the strains.

[0150] (3) Basic training

[0151] Basic medium formula: 1.9% glucose, 2.4% corn steep liquor, 1.6% soybean cake hydrolyzate, 0.2% fish meal, 0.3% potassium dihydrogen phosphate, 0.2% ammonium sulfate, 0.06% magnesium sulfate, 0.08% urea (the above is the mass fraction) , and the balance is distilled wa...

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Abstract

The invention provides a composite microbial inoculum and a preparation method and application thereof. The preparation method of the composite microbial inoculum comprises the steps of performing independent strain activation on strains with an activation culture medium; and mixing activated strains, to obtain mixed seed liquid, and performing fermentation culture with a fermentation culture medium to obtain the composite microbial inoculum, wherein the strains comprise bacillus pumilus, flavobacterium breve, nitrosomonas, rhodospirillum rubrum and nocardin rhodnii. The composite microbial inoculum is prepared by the preparation method of the composite microbial inoculum. The composite microbial inoculum is used for river pollution abatement. The composite microbial inoculum provided by the application forms a stable microbiological system, and repairing and abating pollution of pollution deposit can be realized.

Description

technical field [0001] The invention relates to the field of river pollution control, in particular to a composite bacterial agent and its preparation method and application. Background technique [0002] River sediment pollution is a prominent environmental problem at present. Its pollutants mainly enter the water body through sewage discharge, soil erosion, rainwater leaching and scouring, atmospheric deposition, etc., and are finally deposited in the sediment and gradually enriched, making the sediment heavily polluted. The research on sediment pollution remediation has become a hot spot in the field of river pollution control. [0003] At present, the remediation technologies of river channel polluted sediment mainly fall into three categories: physical remediation, chemical remediation and biological remediation. The use of biotechnology and biological science to remediate polluted river sediments has gradually become a hot spot in sediment pollution control. [0004...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/20C12R1/365C12R1/01C02F103/00
CPCC02F3/341C02F2103/007C12N1/20
Inventor 张新兵胡志鑫廖凯强刘书洪
Owner HUNAN AIRBLUER ENVIRONMENTAL PROTECTION TECH
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