The invention discloses a compound microbiological preparation. The compound microbiological preparation comprises microorganisms and bio-enzyme, wherein the microorganisms contain bacillus subtilis,bacillus licheniformis, nitrosomonas, nitrosospira brieusis, P.Aeruginosa, paracoccus denitrificans, lactobacillus plantarum, lactobacillus casei, candida utilis, saccharomyces cerevisiae, rhodopseudomonas palustris, rhodospirillum, aspergillus niger and enterococcus faecalis; and the bio-enzyme contains microbial enzyme and an aerobic microbial agent. The compound microbiological preparation disclosed by the invention does not contain chemical substances, and secondary pollution to an environment is avoided, and therefore, the compound microbiological preparation is an environmental-friendlymicrobial agent. The preparation process of the compound microbiological preparation has the advantages that the preparation process is simple, the production cost is low, and the compound microbiological preparation has wide prospects on restoration of eutrophicated water bodies.