70 results about "Nitrosomonas sp." patented technology

The invention discloses a microbial agent for treating ammonia-containing waste water and a preparation method thereof. The microbial agent for treating ammonia-containing waste water mainly comprises three bacterial strains of nitrobacter sp, nitrosobacteria nitrosomonas sp., denitrobacter sp., and the contents of the three bacterial strains can be regulated or proportioned according to specificcultivation conditions and product requirements through methods of oriented domestication, and the like. The cultivation method comprises the following steps of: enriching a bacterial source, purifying and amplifying stage by stage for cultivating. The microbial agent can be used for oriented domestication according to a waste water treatment result so as to improve the content of a target bacterial strain. The cultivation method has the advantages of high cultivation efficiency, large production capacity, and the like and is suitable for commercially producing complex microorganisms for treating the ammonia-containing waste water in a large scale. The obtained microbial agent can effectively treat low-COD (Chemical Oxygen Demand) and high-ammonia-nitrogen waste water, such as waste water of catalyst production, and the like.

A wastewater treatment method employing chemicals for disinfection, and precipitation of suspended solids, heavy metals and phosphates before subsequent aerobic bacterial biological treatment using Nitrosomonas and Nitrobacter bacteria to remove ammonia and nitrates / nitrites and BOD compounds to produce recovered treated wastewater suitable for open water discharge.

The invention discloses a culture method of a nitrosomonas sp. flora and a treatment method of waste water containing ammonia nitrogen. The ammonia nitrogen concentration and the pH value of a matrix are increased during an enrichment process, and free NH3 with higher concentration is maintained, thereby gradually achieving the purposes of eliminating nitrobacter sp. and enriching nitrosomonas sp. and obtaining a nitrosomonas sp. flora. Then, the obtained nitrosomonas sp. flora is taken as seeds, the expanded culture is carried out by utilizing self-prepared ammonia nitrogen waste water or high ammonia nitrogen waste water as a culture liquid, the nitrosomonas sp. flora after the expanded culture is inoculated into a biochemical treatment pool for improving the removal efficiency of ammonia nitrogen by biochemical treatment, or the nitrosomonas sp. flora after the expanded culture is directly used for removing the ammonia nitrogen of the ammonia nitrogen waste water, and the optimal proposal is used in the aerobic nitrification stage of the shortcut nitrification-denitrification process for accumulating nitrite nitrogen as an electron acceptor for denitrification directly. The method has simple process and outstanding effect of treating the waste water containing the ammonia nitrogen.

The invention provides a technical method for treating high ammonia nitrogen wastewater by a two-stage A / O process. The two-stage A / O process is divided into four stages, namely an anoxic stage, an aerobic stage, an anoxic stage and an aerobic stage; according to different adaptive conditions of nitrosomonas and nitrobacteria, dissolved oxygen, pH value and sludge age are controlled in an O1 tank to form nitrosomonas advantages, and NH3-N is mainly nitrified into NO2- to realize short range nitration; a part of mixed solution of the O1 tank refluxes to an A1 tank; aqueous carbon source and supplied methanol residue or thin methanol are used as carbon sources for denitrification; methanol residue or thin methanol is supplied in an A2 tank and used as a carbon source for implementing denitrification; high dissolved oxygen is controlled at the O2 stage; and the residual methanol and the rest organic substances in the sewage are further oxidized, and the residual NO2--N is further nitrified to improve the performance of active sludge.

The invention discloses an immobilized microbial preparation which is prepared through the following steps: S1, fermentation culturing strain: respectively inoculating bacillus subtilis, bacillus megatherium, nitrobacter and nitrosomonas in an enrichment medium to be cultured; S2, mixing thallus to obtain mixed thallus liquor; S3, immobilizing PVA-sodium alginate, namely uniformly mixing PVA gel with the mixed thallus liquor, dripping the mixture into a boric acid solution containing CaCl2 while continuously stirring at a low speed, immobilizing and crosslinking the mixture, taking out the mixture, washing the mixture through deionized water, and freeze drying the mixture to obtain the immobilized microbial preparation. The invention further discloses a method of treating meat product processing waste water by utilizing the immobilized microbial preparation. The immobilized microbial preparation disclosed by the invention has the beneficial effects that the microorganism categories are various, various pollutants in the waste water can be degraded, the degrading speed is fast and the capability is strong; the immobilized microbial preparation is suitable for changes of water quality and pH; the sludge yield is small, the products are liable to separate, the stability is good, the technique operation is simple, and the production cost is low.

The invention discloses an ammonia oxidizing bacteria and the application thereof. The ammonia oxidizing bacteria is named as (Nitrosomonas eutropha) CM-NRO14 and is preserved in the China Center for Type Culture Collection (CCTCC for short) of Wuhan University in Wuhan, China on Nov. 12, 2012, the preservation number is CCTCC No: M 2012456. The invention also provides the application of the Nitrosomonas eutropha CCTCC M 2012456 to saprobic ammonia removal, and further provides the application of the Nitrosomonas eutropha CCTCC M 2012456 to water eutrophication treatment. The ammonia oxidizing bacteria has high ammoxidation rate and ammonia removal efficiency and is suitable for high-ammonia nitrogen waste water treatment.

The invention belongs to the technical field of environmental organisms and particularly relates to an autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent for treating ammonia-nitrogen wastewater as well as a culture method and application thereof. The autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent is prepared by mixing culturing nitrosomonas Nitrosomonas sp.N-1, pseudomonas Pseudomonas sp.He-X, thermomonas Thermomonas haemolytica D-2 and achromobacter Achromobacter xylosoxidans C-1 and is used for treating ammonia-nitrogen wastewater. The invention has the advantages of wide adaptation range, strong stability, environmental and toxic impact resistance and the like.

The invention relates to an ammonia-contained waste water high-efficiency biochemical processing method which comprises the following steps of: firstly putting an inoculant into a bioreactor for extensive culture, wherein the inoculant is a microbial flora of Nitrosomonas sp., Denitrobacter sp., Rhodanobacter sp. and the like, and high ammonia nitrogen and high pH value are used as the conditions of the extensive culture; throwing the microbial flora after the extensive culture or a mixture of the microbial flora and aerobic activated sludge of a sewage plant into a nitration reaction pond to process high-ammonia nitrogen low-COD waste water. The processing method has nonstrict requirements for a temperature range and dissolved oxygen concentration. The method not only has the advantages of energy consumption reduction, carbon source saving, sludge production capacity decrease and the like, but also has the characteristics of high volume loading of ammonia nitrogen removal, good processing effect, strong impact resistant capability, stable system operation and the like and is a processing method with stronger synthesis.

The invention aims to provide a microbial flora that can perform degradation of ammonia nitrogen at low temperature. The microbial flora includes Nitrosomonas, Pseudomonas, Bacillus and Nitrobacter. The microbial flora is separated and screened from active sludge and can perform degradation of ammonia nitrogen in sewage at low temperature; when bacterial strain quantity reaches 10<9>-10<11> cfu / ml and initial ammonia nitrogen concentration is 5.0 mg / l, sodium acetate, as a carbon source, is added according to that C / N ratio is 2; under the conditions that temperature is 2 DEG C, pH value is 7.2 and rotation speed is 150 rpm (dissolved oxygen is higher than 2 mg / L), the ammonia nitrogen is degraded for 30 min, wherein degradation rate can reach 9.350 mg(NH4<+>) / L*h. Compared with conventional single bacterial strain, the compound microbial flora can greatly increase the degradation rate of the ammonia nitrogen in sewage. The invention provides a high-quality and high-effective bacterial strain for sewage treatment.

The invention discloses a compound microbial agent which is prepared from nitrifying bacteria, nitrosomonas, single cell bacteria denitrification salt, salt denitrifying bacterium vibrio cholera, achromobacter denitrificans, bacillus thuringiensis and bacillus subtillis, wherein the total viable count is more than 5.9x10<10>cfu / g; the nitrifying bacteria and the nitrosomonas account for 10 to 20 percent of the total viable count; the single cell bacteria denitrification salt, the salt denitrifying bacterium vibrio cholera and achromobacter denitrificans account for 15 to 20 percent of the total viable count; the bacillus thuringiensis and the bacillus subtillis account for 5 to 10 percent of the total viable count. The compound microbial agent has the benefits that the compound microbial agent is fed at a time, and no bacteria is required to be kept added after the system is stabilized; therefore, the operation cost of sewage treatment is reduced, and the convenience of sewage treatment operation is enhanced.

The invention provides a zeolite-calamus constructed wetland sewage disposal system as well as a method utilizing the constructed wetland system to treat domestic sewage. The zeolite-calamus constructed wetland sewage disposal system mainly comprises a tank body, a filler filled in the tank body, aquatic plants planted on the surface of the tank body as well as a water distribution pipe and a water collecting pipe distributed in the tank, the filler is mainly clinoptilolite, Nitrobacter opacus Sack and Nitrosomonas monocella Nelson are put into the filler, and the aquatic plants planted on the surface of the tank body are calami. The system and method adopt clinoptilolite which has good ammonia nitrogen removal effects to serve as the filler of the constructed wetland, in order to improve the treatment efficiency of zeolite, special nitrifying bacteria under artificial culture are screened to regenerate the zeolite, and the constructed wetland system has advantages of high ammonia nitrogen removal capacity, low investment and running costs and simple operation.

The invention discloses nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, a production method and application. Nitrobacter winogradsky is deposited at the China Center for Type Culture Collection with the accession number of ((i) Nitrobacter Winogradsky i ( / i)) Y3-2, CCTCC NO: M2014203. The nitrobacter winogradsky has a function of effectively treating nitrite, and is mixed with nitrosomonas europaea and pseudomonas stutzeri to obtain the complex microbial inoculants, and the complex microbial inoculants can efficiently remove nitrogen in water, and can be used for treating aquaculture water, livestock breeding sewage or industrial sewage.

The invention discloses zeolite modified macromolecule suspension biological carrier based wastewater reinforcement and nitrification process, and belongs to the technical field of sewage treatment. Through the improvement on the core part, a suspension biological carrier, of moving bed biofilm process and biofilm and activated sludge composite process, the suspension biological carrier can have functions of adsorbing ammonia nitrogen and enriching nitrobacteria by adding a functional material; and the zeolite modified macromolecule suspension biological carrier is put into a MBBR / IFFAS reactor according to a certain proportion, so that the enrichment effects of nitrosomonas & nitrobacter on the carrier can be improved, the abundance of functional flora in a reaction system can be enhanced, and therefore, the nitrification effects of the system can be reinforced, and effluent ammonia nitrogen can meet requirements for discharging.

The invention discloses a compound microbiological preparation. The compound microbiological preparation comprises microorganisms and bio-enzyme, wherein the microorganisms contain bacillus subtilis,bacillus licheniformis, nitrosomonas, nitrosospira brieusis, P.Aeruginosa, paracoccus denitrificans, lactobacillus plantarum, lactobacillus casei, candida utilis, saccharomyces cerevisiae, rhodopseudomonas palustris, rhodospirillum, aspergillus niger and enterococcus faecalis; and the bio-enzyme contains microbial enzyme and an aerobic microbial agent. The compound microbiological preparation disclosed by the invention does not contain chemical substances, and secondary pollution to an environment is avoided, and therefore, the compound microbiological preparation is an environmental-friendlymicrobial agent. The preparation process of the compound microbiological preparation has the advantages that the preparation process is simple, the production cost is low, and the compound microbiological preparation has wide prospects on restoration of eutrophicated water bodies.

The invention discloses a microbial agent for sewage treatment. The microbial agent comprises, by weight, 8-10 parts of clostridium acetobutylicum, 12-16 parts of bacillus licheniformis, 16-20 parts of bacillus subtilis, 5-7 parts of lactobacillus acidophilus, 5-7 parts of methanosarcina, 3-5 parts of methanosaeta, 6-8 parts of rhodopseudomonas palustris, 4-6 parts of cellulomonas flavigena, 8-10parts of streptococcus thermophilus, 8-10 parts of alcaligenes faecalis, 3-5 parts of bacillus amyloliquefaciens, 2-4 parts of bacillus nitrosomonas, 1-3 parts of issatchenkia orientalis and 3-5 partsof streptomyces bullis. Besides, the invention further discloses an attachment carrier of the microbial agent and a preparation method of the microbial agent. The microbial agent can be effectively attached to the carrier and accordingly has better activity. The microbial agent is reasonable in strain preparation and can be effectively attached to the attachment carrier to treat sewage, and the method for sewage treatment is simple, good in treatment effect and worthy of popularization.

The invention relates to a microbial inoculum for fermenting and producing organic fertilizers, comprising cellulomonas, bacillus, lactobacillus, methagen, thiobacillus, streptomyces, thermoactinomyces, thermomonospora, azotobacter, nitrosomonas, nitrobacter, rhizobium, aspergillus, yeast, penicillium, humicola, rhizopus and mycorrhizal fungi. In addition, the invention also relates to organic fertilizers fermented and produced by using the microbial inoculum and a method for fermentation and production.

The invention discloses a denitrification composite bacterial agent, a preparation method and an application thereof, wherein the denitrification composite bacterial agent comprises a composite microorganism and an adsorbent; the composite microorganisms consist of the following bacterial fluids in volume ratio: Bacillus megaterium 27-32%, thiobacillus denitrificans 18-23%, Nitrobacteria 18-23%, Nitrosomonas 18-23%, denitrifying bacteria 18-23%. A method for prepare a denitrifying composite bacterial agent comprise fermenting Bacillus megaterium, thiobacillus denitrificans, nitrifying bacteria, nitrosomonas and denitrify bacteria to obtain respective bacterial liquid, mixing and centrifuging according to volume ratio to obtain composite microorganisms, mixing with adsorbent, and freeze drying to obtain denitrifying composite bacterial agent. The denitrification compound bacterial agent prepared by the invention has the advantages of strong environmental adaptability, fast action aging,strong denitrification ability, low cost, good effect, environmental protection and pollution-free, simple preparation, and positive industrial practicability.

A filtration device for an aquarium comprising a filtering chamber for receiving water and a filter medium therein wherein said filter medium comprises fibers having a diameter from 0.1 nm to 3000 nm and an aspect ratio of length to diameter of 5:1 to 10,000 to 1 wherein said fibers provide for colonization of nitrosomonas bacterium and / or nitrobacteria.

A wastewater treatment method employing chemicals for disinfection, and precipitation of suspended solids, heavy metals and phosphates before subsequent aerobic bacterial biological treatment using Nitrosomonas and Nitrobacter bacteria to remove ammonia and nitrates / nitrites and BOD compounds to produce recovered treated wastewater suitable for open water discharge.

The invention discloses a water quality balance ferment for aquaculture and organic sewage treatment and a preparation method thereof. The water quality balance ferment comprises: 10-15% of sugarcane molasses, 0.5-2.0% of milt cake, 10-20% of starch; 0.6-1.5% of a photosynthetic bacteria seed solution, 0.6-1.5% of a Bacillus subtilis seed solution, 0.6-1.5% of a lactic acid bacteria seed solution, 0.6-1.5% of a yeast seed solution, 0.6-1.5% of a Gram positive actinomycete seed solution, 0.6-1.5% of a nitrosomonas seed solution, and 0.6-1.5% of a thiobacillus seed solution; and the balance purified water. The invention also relates to a preparation method and use method of the water quality balance ferment. The quality balance ferment prepared by the method provided by the invention can enhance the resistance of the aquatic products and accelerate growth rate, and after permeating water, can inhibit pathogenic microorganisms and harmful substances, adjust the culture ecological environment, increase the amount of dissolved oxygen in water, promote the activated growth of normal flora and beneficial algae in an aquaculture ecosystem, and keep the ecological balance of the aquaculture water.

The invention relates to nitrobacteria and a culturing method for the nitrobacteria. The nitrobacteria are classified and named to be Nitrosomonas europaea AT7. The culturing method for the nitrobacteria includes the following steps: (1) preparing nitrobacteria basal culturing media, (2) preparing nitrobacteria culturing media containing suspended fillers, (3) seeding the nitrobacteria, and (4) carrying out culturing, and finally obtaining the cultured nitrobacteria. The nitrobacteria and the culturing method have the advantages that compared with the prior art, the culturing time is shortened by 30%-58%, the culturing object bacteria concentration is higher than 1*109 per milliliter and is increased by more than 10 times, intermittent aeration culturing is achieved, and the culturing cost is reduced; in addition, due to the use of the suspended adsorptive objects, when used in still water, the nitrobacteria can stay in an upper-layer water body for a long time, and the effect is better than the effect of dissociative bacteria or other adsorption materials large in density.

The invention relates to a bioremediation preparation of aniline contaminated water. The bioremediation preparation is prepared from a compound bacterium agent and auxiliary materials. The compound bacterium agent is prepared from pseudomonas putida, candida mycoderma bacteria, bacillus amyloliquefaciens, clostridium, nitrosomonas, trichoderma aureoviride and nitrifying bacteria. The strains are reasonable in compatibility, coexist and coordinate with one another, and are not mutually antagonistic. If the preparation is put into a wastewater treatment system, a good degradation effect is achieved on aniline degradation-resistant compounds, the preparation is suitable for industrial sewage treatment, the amount of treated water is increased, the quality of the treated water is improved, operation cost is reduced, and up-to-standard discharge is promoted.

The invention relates to a microorganism electrode preparation method of a water quality toxicity biosensor, which is characterized in that: 40 to 100 parts of freezing and drying colon bacillus, 20 to 80 parts of microzyme and 40 to 60 parts of nitrosomonas are taken according to weight and the three germs are blended to get a blended germ; then 0.3 to 1.0g of blended germ is taken and added into 200 to 500 Mu L of 0.85 percent chlorine solution and a sterilized toothpick is used for mixing well the germ , thus blended germ solution is available; a diprosopia tape is stuck to the round of the working area of the carbon electrode; 5 to 10 Mu L of blended germ solution is drawn with a liquid relief gun, which is smeared to the working area of the carbon electrode and is air-cured for 0.5 to 2.0h in dark under room temperature; then a Makrolan film is covered on the air-cured blended solution, thus the microorganism electrode is made by fixing the germ through the diprosipia tape, which is at last arranged in a fridge of 4 DEG C for 24 to 48h ready for use. The microorganism electrode obtained from the invention improves largely the sensitivity and repetitiveness of the water quality toxicity analysis of the biosensor. The microorganism electrode preparation method of the water quality toxicity biosensor has the advantages of easy techniques and easy operation, can realize the reusage of the biosensor electrode, thus largely reducing the instrument analysis cost.

The present invention is short range nitrifying method realized with inhibitor in aerating biofilter and belongs to the field of sewage treating technology in environment protecting engineering. By using NaClO3 as inhibitor, partial nitrosomonas is preserved while inhibiting nitromonas so as to form short range nitrifying; active sludge is then inoculated into the aerating biofilter and aerated for 3-5 days and water with ammonia nitrogen controlled in 30-80 mg / L is fed and stayed for 1.5-2.5 hr in the air / water volume ratio of 4 to 1 for whole range nitrification; and NaClO3 is added into water to reach 10-20 mmol / L and run for 7-14 days or more time to form short range nitrifying. The present invention realizes short range nitrifying chemically rather by means of strict environment conditions, and has aerating biofilter as reaction carrier and raised short range nitrifying reaction rate.

The present invention refers to a process to increase the concentration of micro organisms' colonies which are formed on the surface of Gramineas bambusoideae in a batch and / or continuous flow process which utilizes biomass as a filtration means in order to remove nitrates as well as organic and inorganic impurities from water and / or from affluents in which a step of adsorption is followed by a step of biological degradation by the anaerobic digestion of micro organisms of the types Pseudonomas SP (Nitrosomonas, Nitrosococus, Nitrobacter, Azobacter, Azotomas and Rhixobium). In accordance to the present invention, the addition of about 200-300 ppm of sodium acetate to the solution fed to the reactor, maintaining a relation of 2:1, C:N, provides an efficiency increase of 80% to 98% regarding the removal of nitrate as well as organic and inorganic soluble matter from the water.

The invention discloses a high-efficiency denitrification compound microbial inoculum, and preparation and an application thereof, and belongs to the field of biotechnology. The microbial inoculum forwastewater treatment mainly includes six strains including nitrobacter sp, nitrosomonas sp, alcaligenes aquatilis, paracoccus nitrovorans, bacillus cereus, providencia vermicola and the like. A culture method includes enrichment, purification and enlarged culture of bacteria; the used microbial inoculum has the advantages of high denitrification efficiency, short domestication time, salt tolerance and the like, and is suitable for large-scale production. The obtained microbial inoculum can effectively treat high ammonia-nitrogen wastewater. The microbial preparation still has good denitrification ability under the condition of 2% total salt, solves the limitation of high-salt environment on a traditional biological denitrification method, and has broad application prospect and good socialbenefits.

The invention discloses an aquaculture pond sediment improver which comprises raw materials in parts by weight as follows: 20-30 parts of construction waste regenerated particles, 5-10 parts of medical stone, 1-5 parts of chitin and 10-20 parts of a probiotics mixed preparation, wherein a nitrobacterium reagent adopts a nitrosomonas, nitrobacter and actinomycetes mixture. According to the improver, the regenerated waste particles having specific particle size after ball milling have excellent adsorption, not only is the problem of construction waste disposal solved effectively, but also a novel improver adsorption subject with better adsorption capacity is provided; the probiotics mixed preparation can effectively promote decomposition of pond bottom sediment, so that the water quality is optimized; the improver after effective compounding of the construction waste regenerated particles, the medical stone, the chitin and the probiotics mixed preparation can be normally used in an aquaculture process, aquaculture animals are not needed to be transferred to another pond, and time is saved; the content of ammoniacal nitrogen, nitrite, hydrogen sulfide and the like in the pond is reduced remarkably after the improver is used for 1-3 days.

The invention discloses a microbial water purifying agent for grass shrimp farming wastewater and a preparation method of the microbial water purifying agent. The preparation method comprises the following steps: adding 30-50 parts by weight of magnetic silica beads with surface modification of C8 and C18 alkyl chains into 300-500 parts by weight of water; adding 150-200 parts by weight of a microbial reagent comprising 40-70 parts of rhodomicrobium, 40-50 parts of rhodobactersphacroides, 20-40 parts of nitrosomonas, 20-30 parts of nitrobacterwinogradskyi, 10-30 parts of pseudomonasstutzeri, 20-40 parts of leptothrix, 4-5 parts of thiobacillus, 2-5 parts of beggiatoa, 50-80 parts of nematodes, 30-40 parts of aeolosomatidaes, 20-40 parts of dunaliella and 20-40 parts of chlorella; and further adding 70-90 parts by weight of a beef extract peptone agar culture medium for culture. By putting the microbial water purifying agent disclosed by the invention into a grass shrimp farming pond, the purpose of purifying the water in the farming pond can be achieved.