Fermented culture medium for producing gibberellic acid

A fermentation medium and technology of gibberellic acid, applied in the direction of fermentation, etc., can solve the problems such as the adverse effects of gibberellic acid on scale and stable fermentation production, affecting the normal production and metabolism of gibberellic acid strains, and the stability of component content cannot be guaranteed. , to achieve the effect of ensuring synthesis and metabolism, ensuring activity and avoiding material loss

Active Publication Date: 2018-05-08
SICHUAN LOMON BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing gibberellic acid fermentation medium is usually based on liquefied starch, peanut cake powder, soybean cake powder, magnesium sulfate and potassium dihydrogen phosphate, among which peanut cake powder and soybean cake powder are excellent organic nitrogen for gibberellic acid fermentation source, but as a by-product of the food industry, the stability of the component content of each batch cannot be guaranteed, which has a negative impact on the large-scale stable fermentation production of gibberellic acid
In addition, peanut cake powder is also easily contaminated with aflatoxin, which affects the normal production and metabolism of gibberellic acid bacteria.

Method used

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  • Fermented culture medium for producing gibberellic acid
  • Fermented culture medium for producing gibberellic acid
  • Fermented culture medium for producing gibberellic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1.1 Put the GA3 strain freezing tube directly into the shake flask, the inoculation amount is 0.8% of the volume of the shake flask medium, the medium formula in the shake flask is shown in Table 1, the fermentation temperature is 29±0.2°C, and the shake flask is cultivated for about 68 hours to obtain a suspension liquid.

[0041] Table 1 shake flask culture medium formula

[0042]

[0043] 1.2 Inoculate the suspension into the primary seed tank with an inoculum volume of 0.016% of the volume of the culture solution. See Table 2 for the medium formula of the primary seed tank.

[0044] Table 2 Primary seed tank culture medium formula

[0045]

[0046] Fermentation volume: constant volume 1.3t, the volume after digestion is calculated as 1.5t (add sulfuric acid before sterilization to adjust the pH to 5.1).

[0047] Fermentation temperature: 29±0.2°C.

[0048] Operating tank pressure: 0.035MPa.

[0049] Air flow: 65Nm 3 / h.

[0050] Transplant time: CO 2 Rise...

Embodiment 2

[0078] 2.1 Transfer the seed solution obtained in 1.3 into a fermenter according to 10% to 15% of the mass of the culture solution for fermentation. The formulation of the fermentation medium is shown in Table 8.

[0079] The formula of table 8 fermentation medium

[0080]

[0081] Fermentation condition is the same as example 1

[0082] The fermentation broth obtained in Example 2 was detected, and the results obtained are shown in Table 9 and Table 10.

[0083] Table 9 fermentation broth detection data

[0084]

[0085]

[0086] Table 10 Wet weight of fermentation broth

[0087]

Embodiment 3

[0089] 3.1 Transfer the seed solution obtained in 1.3 into a fermenter according to 10% to 15% of the mass of the culture solution for fermentation. See Table 11 for the formulation of the fermentation medium.

[0090] The formula of table 11 fermentation medium

[0091]

[0092] The fermentation conditions are the same as in Example 1.

[0093] The fermentation broth obtained in Example 3 was detected, and the results obtained are shown in Table 12 and Table 13.

[0094] Table 12 Fermentation broth detection data

[0095]

[0096] Table 13 Fermentation broth wet weight

[0097]

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PUM

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Abstract

The invention provides a fermented culture medium for producing gibberellic acid. The fermented culture medium comprises 10-40g/L of corn gluten meal, 1-10g/L of monopotassium phosphate, 5-30g/L of asmall molecule organic carbon source, 0.5-5g/L of plant oil, 0.5-2g/L of magnesium sulfate, 0.5-2g/L of ammonium sulfate and 0.1-1g/L of trace elements. Compared with the prior art, the fermented culture medium is relatively high in protein content since the corn gluten meal is adopted as an organic nitrogen source, that is, the protein content is up to 50% or greater, high-density fermentation ofgibberellins is achieved, the potential danger of aspergillus flavus of peanut cake powder is avoided, synthesis and metabolism of bacteria are ensured, and rapid and stable fermentation of the components can be achieved; due to adoption of the plant oil, not only is a carbon source for fermentation provided, but also situations of escape liquids of fermentation bubbles, and the like, are effectively reduced; due to addition of the trace elements in the fermented culture medium, activity of various enzymes and metabolite in the microorganism growth and metabolism process is comprehensively ensured.

Description

technical field [0001] The invention belongs to the technical field of gibberellic acid preparation, in particular to a fermentation medium for producing gibberellic acid. Background technique [0002] Gibberellic acid is a type of secondary metabolite obtained through the fermentation and cultivation of Gibberella. There are 116 species that have been isolated, identified and named, of which GA is the most widely used in agriculture. 3 , plays a huge role in my country's agricultural production. [0003] GA 3 It has very high plant regulation activity, and has obvious regulation effects on the growth and development of various crops. in North, GA 3 It is mainly used in economic crops such as grapes, red dates, hawthorns, nectarines, etc. Spraying a certain concentration of gibberellic acid solution during the flowering period can effectively increase the fruit setting rate of fruit trees, promote fruit growth and development, and increase the yield of fruit trees by abou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P27/00
CPCC12P27/00
Inventor 刘健杨宝强沈颂娣熊仁科齐昊
Owner SICHUAN LOMON BIO TECH CO LTD
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