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Method and apparatus for supplying and recycling oxygen in process of high-density fermentation of aerobic microorganism

An aerobic microorganism and high-density fermentation technology, applied in the field of fermentation engineering, can solve the problems of reduced oxygen utilization, cannot be utilized by microorganisms, and increased cost, and achieves the effects of increasing solubility, shortening aerobic fermentation cycle, and improving productivity.

Inactive Publication Date: 2010-01-06
NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although aerobic microorganisms need a large amount of oxygen in the high-density fermentation process, if a high concentration of oxygen is supplied, most of it (80% or more) will be emptied with the tail gas of the fermentation process so that it cannot be used by microorganisms. This will reduce the utilization rate of oxygen in the fermentation process, and the cost of the fermentation process will be greatly increased due to the introduction of high-concentration oxygen, so it cannot be applied in industry.

Method used

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  • Method and apparatus for supplying and recycling oxygen in process of high-density fermentation of aerobic microorganism
  • Method and apparatus for supplying and recycling oxygen in process of high-density fermentation of aerobic microorganism
  • Method and apparatus for supplying and recycling oxygen in process of high-density fermentation of aerobic microorganism

Examples

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Embodiment 1

[0025] Example 1 Synthesis of polyhydroxybutyrate by high-density aerobic fermentation of Ralstonia eutropha in a 5-liter fermenter

[0026] Strain: Ralstonia eutropha NCIMB 11599 was purchased from the British Culture Collection

[0027] Culture medium composition: the composition of seed culture liquid and fermented liquid is as follows:

[0028]

[0029] Attachment: The trace element solution contains 10g FeSO per liter 4 (7H 2 O), 2.25g ZnSO 4 (7H 2 O), 1.2g CuSO4(5H 2 O), 0.5g MnSO 4 (5H 2 O), 2g CaCl 2 (2H 2 O), 0.25g Na 2 B 4 o 7 (7H 2 O), 0.12g (NH 4 )6Mo 7 o 24 , and 10ml of 35% HCl.

[0030] Analysis and test method: take out 2 ml of fermentation broth during the fermentation process, after centrifugation, washing, and centrifugation again, dry the cells to constant weight at 105°C, weigh them to calculate the cell concentration: dry cell weight / per liter of fermentation broth. The concentration of the metabolite polyhydroxybutyrate (PHB) was meas...

Embodiment 2

[0035] Example 2 Synthesis of polyhydroxybutyrate by high-density aerobic fermentation of Ralstonia eutropha in a 30-liter fermenter

[0036] Adopt the bacterial classification identical with embodiment 1, culture medium composition, analytical test method and process control method, obtain following result in 30 liters of fermentors:

[0037] As the volume of the fermentation tank increases, the number of microorganisms in the tank increases, requiring more oxygen to meet the needs of the growth of aerobic microorganisms and the metabolism and synthesis of products; Ester is carried out in 30 liters fermentors, keeps other all operating conditions identical with embodiment 1, but the gas that fermentor 3 gas outlets discharges is sent into moisture separator 5 earlier, then enters second oxygen generator II4; Utilize Oxygen supply and recycling method in the high-density fermentation process of aerobic microorganisms, the final cell concentration and product concentration are...

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Abstract

The invention discloses a method and an apparatus for supplying and recycling oxygen in a process of high-density fermentation of aerobic microorganism. In the aerobic microorganism fermentation process, air is used as a gas source to prepare oxygen with the high density of 50 and 90 percent through a first oxygen generator I (1), and the oxygen with the high density of 50 and 99 percent is stored in a gas storage tank (2), and continuously aerated into an aerobic microorganism fermentation tank (3) by a regulated flow rate, and the first oxygen generator I(1) continuously uses the air as the gas source to prepare the oxygen with the high density; and the gas exhausted from an outlet of a fermentation tank (3) is led into a second oxygen generator II (4) to recycle the oxygen from the second oxygen generator II, and the gas is mixed with the oxygen with the high density prepared by the first oxygen generator I (1), and the gas is again led into the fermentation tank (3) to be circularly used in the fermentation process. The method and the apparatus can improve the production ratio of the aerobic fermentation process and reduce the production cost.

Description

technical field [0001] The invention relates to a method and equipment for oxygen supply, recovery and reuse in the high-density fermentation process of aerobic microorganisms, and belongs to the technical field of fermentation engineering. Background technique [0002] In the process of aerobic fermentation, the biological oxidation process of microorganisms requires sufficient oxygen supply. Insufficient oxygen supply will seriously affect the growth of aerobic microorganisms and the synthesis of metabolites, and microorganisms can only use oxygen dissolved in the liquid phase. In order to improve productivity, it is necessary to make "oxygen supply greater than oxygen consumption" throughout the fermentation process to eliminate the impact of insufficient oxygen supply. During the fermentation process, the oxygen consumption will increase with the growth and metabolic rate of microorganisms and the concentration of microorganisms in the fermenter. However, oxygen is a ga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12M1/04
CPCC12M41/34C12M29/24
Inventor 尚龙安张艳辉任红姜岷张虎南
Owner NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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