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Yeast cell immobilizing method

A technology for yeast cells and immobilized yeast, which is applied in the field of immobilized yeast cells, and achieves the effects of low price, strong operability, and simple immobilization method

Inactive Publication Date: 2007-01-03
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[10-11] However, all these methods cannot fundamentally solve the main problems of calcium alginate

Method used

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  • Yeast cell immobilizing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. Using sweet sorghum slag as a carrier for immobilized yeast fermentation to produce alcohol

[0024] 1. Materials:

[0025] 1 strain: Saccharomyces cerevisiae was purchased from the Beijing market

[0026] 2 sweet sorghum stalks: from Hebei

[0027] 3 media:

[0028] (1) Test tube slant solid medium: sugar 20g / L, yeast powder 3g / L, peptone 5g / L, wort juice 3g / L, agar 20g / L, pH 3.5-7.

[0029] (2) Liquid medium: sugar 20g / L, yeast powder 3g / L, peptone 5g / L, wort 3g / L, pH 3.5-7.

[0030] (3) Proliferation medium: glucose 150g / L, KH 2 PO 4 0.5g / L, peptone 5g / L, MgSO 4 1g / L, pH 3.5-7.

[0031] (4) Fermentation medium: glucose 50g / L, sucrose 100g / L, fructose 50g / L, KH 2PO 4 0.5g / L, peptone 5g / L, MgSO 4 lg / L.

[0032] (5) Instruments and equipment: electric furnace, constant temperature incubator, shaker, microscope.

[0033] 2. Method

[0034] 1. Preparation of mother liquor: Bacteria culture: Take a test tube slant, insert it into a 250ml Erlenmeyer ...

Embodiment 2

[0038] Example 2. Using sweet sorghum stalks as a carrier for immobilized yeast fermentation to produce alcohol

[0039] 1. Materials:

[0040] 1 strain: Saccharomyces cerevisiae was purchased from the Beijing market

[0041] 2 sweet sorghum stalks: from Hebei

[0042] 3 media:

[0043] (1) Test tube slant solid medium: sugar 20g / L, yeast powder 3g / L, peptone 5g / L, wort juice 3g / L, agar 20g / L, pH 3.5-7.

[0044] (2) Liquid medium: sugar 20g / L, yeast powder 3g / L, peptone 5g / L, wort 3g / L, pH 3.5-7.

[0045] (3) Proliferation medium: glucose 150g / L, KH 2 PO 4 0.5g / L, peptone 5g / L, MgSO 4 1g / L, pH 3.5-7.

[0046] (4) Fermentation medium: glucose 50g / L, sucrose 100g / L, fructose 50g / L, KH 2 PO 4 0.5g / L, peptone 5g / L, MgSO 4 1g / L.

[0047] (5) Instruments and equipment: electric furnace, constant temperature incubator, shaker, microscope.

[0048] 2. Method

[0049] 1. Preparation of mother liquor: Bacteria culture: Take a test tube slant, insert it into a 250ml Erlenmey...

Embodiment 3

[0053] Example 3. Using bagasse as a carrier for immobilized yeast fermentation to produce alcohol

[0054] 1. Materials:

[0055] 1 Strains: Pachysolen tannophilus Microbial Food Fermentation Institute

[0056] 2 Purple sugar cane: purchased from Guangxi

[0057] 3 media:

[0058] (1) Test tube slant solid medium: sugar 20g / L, yeast powder 3g / L, peptone 5g / L, wort juice 3g / L, agar 20g / L, pH 3.5-7.

[0059] (2) Liquid medium: sugar 20g / L, yeast powder 3g / L, peptone 5g / L, wort 3g / L, pH 3.5-7.

[0060] (3) Proliferation medium: glucose 150g / L, KH 2 PO 4 0.5g / L, peptone 5g / L, MgSO 4 1g / L, pH 3.5-7.

[0061] (4) Fermentation medium: glucose 50g / L, sucrose 100g / L, fructose 50g / L, KH 2 PO 4 0.5g / L, peptone 5g / L, MgSO 4 1g / L.

[0062] (5) Instruments and equipment: electric furnace, constant temperature incubator, shaker, microscope.

[0063] 2. Method

[0064] 1. Preparation of mother liquor: Bacteria culture: Take a test tube slant, insert it into a 250ml Erlenmeyer fl...

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Abstract

The present invention relates to yeast cell immobilizing method with plant stalk or squeezed residue as carrier. The yeast cell immobilizing method includes the following steps: 1. crushing or squeezing plant stalk to eliminate its water, and sterilizing; 2. inoculating yeast cell to liquid culture medium, mixing the liquid culture medium with yeast cell and the sterilized plant stalk or squeezed residue for solid or semi-solid culturing; and 3. adding multiplying culture medium and further multiplying culture.

Description

technical field [0001] The present invention relates to a method for immobilizing yeast cells, in particular to a method for immobilizing yeast cells in which plant stems or residues left after pressing are used as carriers. Background technique [0002] In the fermentation process of alcohol and beer production, the use of immobilized yeast is a new biotechnology developed from immobilized enzyme technology in the 1970s. Since the immobilized yeast can increase the cell density of the fermentation system by several times or even dozens of times, and is suitable for operation at a high dilution rate, it can greatly increase the fermentation speed, shorten the fermentation period, improve the production capacity and production efficiency of the equipment, and simplify The production process reduces labor intensity and makes the production process more stable and easy to control. Due to the above advantages, immobilized yeast technology has beco...

Claims

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Application Information

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IPC IPC(8): C12N11/16C12N1/16
Inventor 谭天伟俞建良张栩
Owner BEIJING UNIV OF CHEM TECH
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