32results about How to "Immobilization method is simple" patented technology

The invention discloses the preparation method of the ultra fine fiber membrane of an affinity acrylonitrile base copolymer. The acrylonitrile base copolymer is dissolved in solvent to form solution and then electrostatic spinning is conducted to prepare the fiber membrane. The mass percentage of the acrylonitrile base copolymer in the solution is two to eight percent; the spinning voltage of the electrostatic spinning is six to twenty five kv; the flow velocity of spinneret solution is 0.5 to 2.0 ml per hour with the deposition distance ranging from eight to twenty centimeters. The acrylonitrile base copolymer is acrylonitrile/allylamine copolymer, acrylonitrile/methyl allylamine copolymer, acrylonitrile/acrylamide copolymer or acrylonitrile/methacrylamide copolymer with the viscosity-average molecular weight being eighty to three hundred thousand; the solvent is one or various kinds which are mixed with random percentages of dimethyl sulfoxide, dimethyl formyl ammonia or dimethyl acetyl ammonia. After the glycan of the fixed shell of a chemical bond at the surface of the membrane, the membrane is used for separating and purify protein with simple operation, good chemical stability and low cost.

The invention provides a radioactive wastewater treatment method. The radioactive wastewater treatment method provided by the invention comprises the following steps: adding active calcium silicate to radioactive wastewater with the pH value of greater than or equal to 2, and then performing solid-liquid separation to obtain purified water and radioactive calcium silicate, wherein the grain diameter of the active calcium silicate is smaller than 50 [mu]m, the bore diameter is 2-50 nm, and the specific surface area is 400-700 m<2>/g. According to the radioactive wastewater treatment method provided by the invention, the active calcium silicate is taken as a treating agent, the active calcium silicate is not easy to be influenced by the water quality and pH value of wastewater when the radioactive wastewater is treated, and radioactive elements and other metal ions in the radioactive wastewater can be effectively removed under a smaller additive amount; and moreover, an immobilization method for the radioactive calcium silicate formed after treatment is simple, and the immobilization effect is good.

The invention discloses an immobilized recombinant penicillin G acylase and an application thereof to preparation of (S)-2-aryl-amino acid and cephalosporin antibiotics. The immobilized recombinant penicillin G acylase disclosed by the invention is capable of converting an S-type substrate into an S-type product within shorter time, high in substrate tolerance and strict in S selectivity for the substrate and structural analogues thereof; due to the addition of cobalt ions as well as a protective agent with phenylacetic acid and glycerin as enzyme active centers, the immobilized recombinant penicillin G acylase disclosed by the invention is prevented from being successfully subjected to multipoint covalent immobilization on the surface of an epoxy resin carrier under the condition that enzyme molecules are seriously inactivated in an immobilization process, and the immobilization method is simple and feasible, cheap in raw materials and suitable for large-scale operation; the immobilized recombinant penicillin G acylase can be used for synthesizing cephalosporin antibiotics and splitting the prepared (S)-2-aryl-amino acid, and can be continuously used for more than 50 batches before the activity of the immobilized enzyme is not remarkably reduced.

The invention discloses an L-lactic acid fermentation method by using polyvinyl alcohol to solidify rhizopus oryzae. The method comprises the following steps: heating polyvinyl alcohol and sodium alginate in a boiling water bath to be jointly dissolved; mixing with a rhizopus oryzae spore suspension; using an injector to drip the mixed solution to a saturated boric acid solution containing calcium chloride while using a magnetic stirrer to continuously stir so that particles are spherical and good immobilized particles can be hardened at a low temperature; and then, treating by a monobasic sodium phosphate solution and washing with distilled water after solidified cells are propagated and grow so as to be used for the batch fermentation and the repetitive batch fermentation of the L-lactic acid. The improved L-lactic acid fermentation method by using the polyvinyl alcohol to solidify the rhizopus oryzae overcomes the defects of spherical carriers and poor permeability of the prior polyvinyl alcohol and has the advantages of great mechanism strength, large acid production amount, high acid production speed and good repetitive use effect.

The invention discloses an immobilized glutamate decarboxylase and a preparation method thereof. The immobilized glutamate decarboxylase is prepared according to the method comprising the following steps of: 1) adding acetic acid-acetate buffer solution into lactic acid bacteria to obtain bacterial suspension containing 300-500cfu bacteria per milliliter, performing ultrasonic crushing on the bacterial suspension under the condition of an ice-water bath to obtain crude enzyme solution of the glutamate decarboxylase; 2) using cotton flannel fabric as a carrier, and sequentially performing pretreatment with sodium hydroxide, pyridine and para-toluenesulfonyl chloride; and 3) arranging the cotton flannel fabric after the pretreatment in the crude enzyme solution of the glutamate decarboxylase for soaking, adding glutaraldehyde into the solution, and realizing immobilization of the glutamate decarboxylase. The immobilization method of the glutamate decarboxylase is simple, the conditions are required to be mild and easy to implement, and the obtained immobilized glutamate decarboxylase has the advantages of high activity, large loading amount, long catalytic reaction service life and the like.

The invention discloses a method for preparing a series of neoagarool igosaccharode with single degree of polymerization by utilizing an immobilized enzyme. The method comprises the following steps: (1) acquiring agarase powder from vibrio natriegens HJPHYXJ-1; (2) preparing an immobilized carrier; (3) preparing the immobilized enzyme; (4) preparing a series of neoagarool igosaccharode. Accordingto the method, an immobilized agarase is prepared by adopting an immobilized enzyme technology and is applied in the preparation of the neoagarool igosaccharode; through simple magnetic separation, reutilization of the agarase can be realized, and aftertreatment steps are simplified, therefore, the production cost of the neoagarool igosaccharode is greatly lowered; additionally, the immobilized carrier used in the method is simple to prepare, and an immobilization method is simple and effective, so that the theoretical and technical supports can be provided for future industrial large-scale production of the neoagarool igosaccharode.

The invention provides an enzyme membrane reactor. The enzyme membrane reactor comprises a microfiltration membrane and an immobilized enzyme, wherein the immobilized enzyme comprises a horseradish peroxidase which is fixed to the microfiltration membrane through an embedding medium. The invention also provides a method of using the enzyme membrane reactor to treat wastewater. The enzyme membranereactor and method have the advantages that the enzyme membrane reactor has high reaction activity and fast reaction speed and can basically complete reaction in ten minutes; reaction conditions are mild, and the reactor operates at a normal temperature and a normal pressure; an enzyme immobilization method is simple, and the production cost is low; an enzyme membrane has good stability, an enzymeis unlikely to run off, the membrane is unlikely to be blocked, and the service life is long; the enzyme membrane reactor can treat various pollutants such as phenols and azo, and has a wide application range; only a small amount of hydrogen peroxide is added, and the treatment cost is low; and no poisonous and harmful chemical agents are added, and thus, the secondary pollution of environments is avoided.

The invention provides a production method of inosinic acid. The method comprises the step of adopting adenosine deaminase fixed in anion resin to catalyze the generation of the inosinic acid from adenylic acid. According to the method provided by the invention, the adenylic acid is taken as a substrate, the supply of raw materials is sufficient, the requirements for equipment are low, the separation and extraction are convenient, the semi-continuous production of the inosinic acid can be realized, the substrate is convenient and easy to obtain, the transformation is thorough, and the cost is relatively low.

An immobilized organism co-metabolism and decolouring process for the dyeing sewage includes such steps as adding nutritive liquid to non-sterilized water to obtain bacterial suspension, adding it to an up-flow fixed bed reactor filled with attapulgite, and adding carbon source and nitrogen source substances to the sewage to be treated. Its advantage is high average decolouring rate up to 80%.

The invention relates to a preparation method of immobilized water purifying agent, which comprises the following steps: mixing immobilized carrier and composite microbial agent according to a volumeratio of 1: (6-7), stirring and culturing at 28-30 DEG C for 36-48h. The composite microbial agent comprises of acinetobacter, candida mycoderma bacteria and bacillus subtilis. The water purifying agent can obviously reduce the COD value of the eutrophic water body and inhibit the large-scale growth of the water hyacinth, thereby having popularization value and wide application prospect.