Method for preparing immobilized particles for rehabilitation of soil polluted by polycyclic aromatic hydrocarbon

A technology for contaminated soil and polycyclic aromatic hydrocarbons, which is applied in the field of adsorption and immobilization of microorganisms and preparation of immobilized particles, to achieve the effects of enhanced mass transfer, shortened preparation cycle, and simple and easy immobilization method

Inactive Publication Date: 2008-11-05
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports at home and abroad on the use of immobilized microorganisms for the degradation of organic pollutants in non-fluid media (soil).

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Incline culture of immobilized bacteria

[0026] In the medium containing inorganic salts (sucrose 4.0%, (NH 4 ) 2 HPO 4 0.4%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, Vitamin B 1 0.005%, pH 6.5) to inoculate mucormycetes into test tubes, and place them in an incubator at 28-30°C for 3 days.

[0027] (2) Preparation of original seed

[0028] Select fresh corn kernels without mildew, boil them for 30 minutes, put them into a culture bottle, seal them with polyethylene film plastic, and sterilize them under high pressure at 121°C for 90 minutes. Insert fungal spores on the slant culture medium, seal it, put it in a constant temperature incubator at 30°C, take it out when the corn kernels are covered with off-white hyphae, and set aside.

[0029] (3) Pretreatment of immobilized carrier

[0030] Dissolve fresh, dry corncobs that have been crushed or crushed to a size of 1.5-2cm, 1% quicklime in water, pour them into the material, soak them well, cover them with a...

Embodiment 2

[0037] One of the differences with Example 1 is: when the original seed was prepared, the formula was 99% corn kernels, 1% gypsum, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, urea 0.8%, after high temperature steam sterilization, access to spore suspension, 1×10 4 Spores / ml, the original species can be obtained after 10 days of constant temperature cultivation at 28°C;

[0038] The second difference is: in the pretreatment process of the carrier, the auxiliary material composition is changed from rice bran to wheat bran, that is, 80% corn cob, 20% wheat bran, 1% gypsum, (NH 4 ) 2 HPO 4 2%, white sugar 1%, MgSO 4 ·7H 2 O0.1%, quicklime 1-2%, high-pressure steam sterilization twice, the first time, 121°C, 60min, the second time, 121°C, 30min, after cooling, insert the original seed, seal, and cultivate at room temperature, The daytime temperature is 25-28°C, and the nighttime temperature is 15-20°C. After the mycelium penetrates the corn cob carrier and fills the culture bott...

Embodiment 3

[0042] The difference from Example 1 is that there is no original seed preparation process, but the mother seed is directly used for immobilization. In the preparation process of the carrier, the corn cob is not softened, and is directly mixed with auxiliary materials to prepare the carrier. The results showed that although the immobilized granules prepared by this method shortened the time by 5 days due to the absence of the culture stage of the original species, the culture and proliferation time of the immobilized granules was prolonged by 11 days, and the bottle was full in 35 days, and the number of spores of the immobilized granules Less, 1×10 4 pcs / g.

[0043] The immobilized particles prepared by this method remediate the soil polluted by polycyclic aromatic hydrocarbons according to the 10% particle ratio. After 56 days, the removal rate of PYR is 70%, and the removal rate of BaP is only 30%.

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PUM

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Abstract

The invention relates to the cell immobilization technology, specifically speaking, it's about the preparing method of a solidified granule which can be used in the repair of soil which is polluted by polycyclic aromatic hydrocarbon. The main carrier is corncob. Mix the fresh, dry and pulverized corncob (the diameter is about 1.5-2cm) with cotton kernel pastry, wheat bran, bean cake powder, rice bran and / or wheat bran, of which the corncob accounts for 70-85%, the accessories account for 15-30%; regulate the water content to 40-55wt% and pH value to 6.3-6.5; after autoclaving, inoculate the foundation stock full of fungal spore and enrichment cultivate to get the solidified granule. The advantage of the invention includes: low cost, high solidifying efficiency, simple operating method and suitable to repair the soil in large scale.

Description

technical field [0001] The invention relates to cell immobilization technology, in particular to a method for preparing immobilized granules for repairing polycyclic aromatic hydrocarbon contaminated soil. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to a class of compounds with two or more benzene rings fused together, and they are ubiquitous in the environment. Human activities, especially the burning of fossil fuels, are the main source of PAHs in the environment. Due to the high octanol-water partition coefficient and poor water solubility of these compounds, they are often adsorbed on soil particles. Therefore, soil becomes the main carrier of PAHs. PAHs are relatively stable in soil and difficult to decompose. Even if the method of dealing with volatile and easily degradable organic pollutants in soil is more successful, it is difficult to remove PAHs in soil. The accumulation of polycyclic aromatic hydrocarbons in nature will cause gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/00C12N11/16C12R1/645B09C1/10
Inventor 苏丹李培军鞠京丽
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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