Preparation method of immobilized water purifying agent and application thereof for treating aquatic herbs

A technology of water purifier and immobilized carrier, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. With the water loss and other problems, to achieve the effect of strong practical value, convenient preparation, and reduce the dosage

Inactive Publication Date: 2018-09-04
杨敏杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has a certain effect in a short period of time, but because the free bacteria are not easy to colonize in the water body, the activity is not

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] A fixed water purifying agent, which comprises a compound microbial preparation and an immobilized carrier.

[0024] The composite microbial agent is composed of heterotrophic nitrifying bacteria-Acinetobacter, Candida and Bacillus subtilis.

[0025] The preparation method of the composite microbial inoculum is: cultivating Acinetobacter, Candida, and Bacillus subtilis to a concentration of 1×10 according to a conventional culture method. 7 Bacteria / ml, and then mix according to the volume ratio of 3:1:2 to obtain;

[0026] The Acinetobacter SP. is ATCC NO.17978;

[0027] The Candida is (Candida utilis) ATCC NO.22023;

[0028] The Bacillus subtilis is (Bacillus subtilis) ATCC NO.6633

[0029] The immobilized carrier is composed of ceramsite and zeolite in a weight ratio of 1:1;

[0030] The immobilized carrier and the composite microbial inoculum were mixed according to a volume ratio of 1:6, and cultured for 48 hours under aeration (stirring speed of 120rpm / Min) ...

Example Embodiment

[0032] Example 2 An immobilized water purifying agent, which includes a composite microbial preparation and an immobilized carrier.

[0033] The composite microbial agent is composed of heterotrophic nitrifying bacteria-Acinetobacter, Candida and Bacillus subtilis.

[0034] The preparation method of the compound microbial inoculum is: cultivating Acinetobacter, Candida, and Bacillus subtilis to a concentration of 1×10 according to a conventional culture method. 7 Pieces / ml of bacterial liquid, and then mix according to the volume ratio of 4:2:3 to obtain;

[0035] The Acinetobacter SP. is ATCC NO.17978;

[0036] The Candida spp. is (Candida utilis) ATCC NO.22023;

[0037] The Bacillus subtilis is (Bacillus subtilis) ATCC NO.6633

[0038] The immobilized carrier is composed of ceramsite and zeolite in a weight ratio of 1:1;

[0039] The immobilized carrier and the composite microbial inoculum were mixed in a volume ratio of 1:7, and cultured for 48 hours under aeration (stirring speed of 1...

Example Embodiment

[0041] Example 3 The pathogenicity test of the water purifying agent of the present invention on water hyacinth:

[0042] (1) Preparation of compound microbial agent:

[0043] Experimental group: Acinetobacter, Candida, and Bacillus subtilis were cultivated to a concentration of 1×10 according to conventional culture methods. 7 Cells / ml, and then mix according to the volume ratio of 3:1:2; the Acinetobacter SP. is ATCC NO.17978; the Candida utilis is ATCC NO.22023 ; The Bacillus subtilis is (Bacillus subtilis) ATCC NO.6633;

[0044] Control group 1: Acinetobacter and Candida were cultivated to a concentration of 1×10 according to conventional culture methods. 7 Cells / ml, and then mix according to the volume ratio of 3:1; the Acinetobacter is ATCC NO.17978; the Candida is ATCC NO.22023;

[0045] Control experiment group 2: Cultivate Candida and Bacillus subtilis to a concentration of 1×10 according to conventional culture methods. 7 Cells / ml, and then mix according to the volume ratio ...

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PUM

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Abstract

The invention relates to a preparation method of immobilized water purifying agent, which comprises the following steps: mixing immobilized carrier and composite microbial agent according to a volumeratio of 1: (6-7), stirring and culturing at 28-30 DEG C for 36-48h. The composite microbial agent comprises of acinetobacter, candida mycoderma bacteria and bacillus subtilis. The water purifying agent can obviously reduce the COD value of the eutrophic water body and inhibit the large-scale growth of the water hyacinth, thereby having popularization value and wide application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of an immobilized water purifying agent and its application for treating aquatic herbs. Background technique [0002] Water hyacinth (Eichhornia crassipes Solms) is a perennial aquatic herb of the genus Eichhornia crassipes, also known as water hyacinth, false narcissus, and foreign narcissus. It is native to South America and was introduced to my country in the 1930s. Promoted as livestock and poultry feed. Water hyacinth begins to reproduce at a temperature of 13°C, and can still reproduce at a temperature of 39°C. It has both asexual and sexual reproduction functions, especially asexual reproduction, and its population recovery and diffusion capabilities are extremely strong. Water hyacinth likes to be born in warm, sunny and still water rich in organic matter. Under suitable conditions, a new plant can be reproduced every 5 days, and the biomass can increase ...

Claims

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Application Information

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IPC IPC(8): C02F3/34
CPCC02F3/34
Inventor 杨敏杰卢珍晶沈华平李震宇
Owner 杨敏杰
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