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Method for preparing gamma-aminobutyric acid by fermenting agricultural by-products with lactic acid bacteria

A technology of lactic acid bacteria fermentation, agricultural and sideline products, applied in the direction of fermentation, fixed on/in organic carriers, etc., can solve the problem of difficulty in obtaining microbial strains

Active Publication Date: 2020-11-13
浙江益弘食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its products have the advantages of low cost, high content and safe use in food, but efficient microbial strains are usually difficult to obtain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The method for preparing gamma-aminobutyric acid by fermenting agricultural by-products with lactic acid bacteria comprises the following steps:

[0036] S1, preparation of lactic acid bacteria suspension:

[0037] Inoculate the lactic acid bacteria in the liquid enrichment medium, culture on a shaker to obtain the bacteria in the logarithmic growth phase, after centrifugation, wash the bacteria with normal saline for 3 times, collect by centrifugation again, and dilute with normal saline to obtain a suspension of lactic acid bacteria liquid, the number of live bacteria in the lactic acid bacteria suspension is 10 8 CFU / ml.

[0038] S2, immobilization of lactic acid bacteria:

[0039] Take 15ml of lactic acid bacteria suspension, add 1.2g sericin, and mix thoroughly to obtain liquid A; spray liquid A onto 1.8g carboxymethyl cellulose to obtain mixed liquid B; add 0.4g polyaldehyde group-containing oxidized dextran Add it into liquid B, vibrate and cross-link at 37°C at...

Embodiment 2

[0044] The method for preparing gamma-aminobutyric acid by fermenting agricultural by-products with lactic acid bacteria comprises the following steps:

[0045] S1, preparation of lactic acid bacteria suspension:

[0046] Inoculate the lactic acid bacteria in the liquid enrichment medium, culture on a shaker to obtain the bacteria in the logarithmic growth phase, after centrifugation, wash the bacteria with normal saline for 3 times, collect by centrifugation again, and dilute with normal saline to obtain a suspension of lactic acid bacteria liquid, the number of live bacteria in the lactic acid bacteria suspension is 10 8 CFU / ml.

[0047] S2, immobilization of lactic acid bacteria:

[0048] Take 10ml of lactic acid bacteria suspension, add 1.0g of sericin, and mix thoroughly to obtain liquid A; soak cellulose acetate in deionized water for 35 hours to obtain swollen cellulose, and then stir the swollen cellulose and solution A in a water bath at 37°C 1-2h to obtain mixed l...

Embodiment 3

[0053] The method for preparing gamma-aminobutyric acid by fermenting agricultural by-products with lactic acid bacteria comprises the following steps:

[0054] S1, preparation of lactic acid bacteria suspension:

[0055] Inoculate the lactic acid bacteria in the liquid enrichment medium, culture on a shaker to obtain the bacteria in the logarithmic growth phase, after centrifugation, wash the bacteria with normal saline for 3 times, collect by centrifugation again, and dilute with normal saline to obtain a suspension of lactic acid bacteria liquid, the number of live bacteria in the lactic acid bacteria suspension is 10 9 CFU / ml.

[0056] S2, immobilization of lactic acid bacteria:

[0057] Take 12ml of lactic acid bacteria suspension, add 1.6g of sericin, and mix thoroughly to obtain liquid A; soak DEAE cellulose in deionized water for 30 hours to obtain swollen cellulose, and then stir the swollen cellulose and solution A in a water bath at 37°C 2h to obtain mixed liquid...

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Abstract

The invention provides a method for utilizing a lactobacillus fermented agricultural and sideline product to prepare gamma-aminobutyric acid, and belongs to the technical field of biological processing of agricultural and sideline products. The method comprises the steps that S1, lactobacillus bacterial suspension liquid is prepared; S2, lactobacillus is fixed; and S3, the fixed lactobacillus obtained in the step S2 is inoculated to a liquid culture medium containing the agricultural and sideline product and sufficiently stirred, and the liquid ferments. Lactobacillus is fixed by utilizing sericin, cellulose and oxidized dextran containing polyaldehyde, and the lactobacillus fermented agricultural and sideline product has the characteristics that the biocompatibility is high, the product is safe and non-toxic, the stability is good, and multiplication of lactobacillus is promoted. The waste agriculture and sideline product is adopted to be a fermentation culture substrate, resource recycling is achieved, and the obtained fermented liquid contains the nutrient matter in the agricultural and sideline product and a great amount of gamma-aminobutyric acid, and has the very high nutrient value.

Description

technical field [0001] The invention belongs to the technical field of biological processing of agricultural and sideline products, and in particular relates to a method for preparing gamma-aminobutyric acid by using immobilized lactic acid bacteria to ferment agricultural and sideline products. Background technique [0002] γ-Aminobutyric acid (GABA), also known as aminobutyric acid, is a white crystalline powder, non-protein amino acid widely distributed in animals and plants, and is the main inhibitory neurotransmitter of the central nervous system. GABA has important physiological functions: (1) Improve memory and enhance memory function; (2) Promote the secretion of growth hormones. Growth hormones can promote the strengthening of skeletal muscles, improve immunity, reduce fat, and improve body functions; (3) Analgesic, sedative and sleep-improving effects; (4) Relieving and treating hypertension. [0003] The preparation methods of γ-aminobutyric acid mainly include c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/12C12N11/02C12P13/00
CPCC12N11/02C12N11/12C12P13/005
Inventor 刘士旺方理明龚金炎肖功年楼坚柳永鲍文娜楚秉泉张亚青
Owner 浙江益弘食品有限公司
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