Method for producing immobilized bacterium with peat soil as carrier

A peat soil and carrier technology, which is applied in the direction of being fixed on or in an inorganic carrier, to achieve the effects of simple and easy method, increased resistance and enhanced competitiveness

Inactive Publication Date: 2009-11-18
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports at home and abroad on the use of immobilized microorganisms for the degradation of organic pollutants in non-fluid media (soil).

Method used

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  • Method for producing immobilized bacterium with peat soil as carrier
  • Method for producing immobilized bacterium with peat soil as carrier
  • Method for producing immobilized bacterium with peat soil as carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Liquid culture of immobilized bacteria

[0023] In seed medium (glucose 1.25%, beef extract 0.25%, NH 4 NO 3 0.1%, MgSO 4 ·7H 2 O 0.02%, KCl 0.02%, pH 6.5) Erlenmeyer flasks were inoculated with Bacillus, and placed in a constant temperature shaker at 28-30°C for 12 hours.

[0024] (2) Alkalization treatment of immobilized carrier

[0025] Pass the natural air-dried peat soil through a 2mm sieve, soak it in 0.1mol / L NaOH solution, solid-to-liquid ratio 1:10, shake on a shaking table for 24 hours, and rotate at 110rpm; then take it out and adjust the pH to 7.0 with 1mol / L NaOH solution, and then centrifuge. 3000rpm, 15min; remove the supernatant, dry the precipitate at 60°C, and grind it through a 1mm sieve.

[0026] (3) Immobilized carrier infiltration culture

[0027] Add sterile water to the alkalized peat soil at a ratio of 1ml / g, sterilize with high temperature and humidity for 90 minutes, and set aside.

[0028] (4) Preparation of immobilized particles ...

Embodiment 2

[0034]One of the differences from Example 1 is: the carrier alkalization treatment step is to pass the natural air-dried peat soil through a 2mm sieve, neutralize the peat sample to pH 7.0 with 1mol / L NaOH, and then use 0.15mol / LNaOH to adjust the solid-liquid ratio to 1:5, shaking on a shaking table for 12 hours at a speed of 110 rpm, centrifuging at 3000 rpm for 10 minutes, drying the precipitate at 80°C, and grinding it through a 1mm sieve.

[0035] The second difference is that the carrier does not have an infiltration treatment step after alkalization, but directly sterilizes the dried peat soil, then inoculates, proliferates and cultures for 3 days, and obtains immobilized particles.

[0036] The third difference is that the immobilized granules have no proliferation and culture process. Take the bacterial suspension, add it evenly to the surface of the sterile carrier, inoculate 2%, seal it, put it in a constant temperature incubator at 28°C and incubate it in the dark ...

Embodiment 3

[0040] The difference from Example 1 is that: the carrier alkalization treatment method is to pass the natural air-dried peat soil through a 2mm sieve, soak it with 0.05mol / L NaOH solution, the solid-to-liquid ratio is 1:8, and vibrate on a shaking table for 18h at a speed of 110rpm; then Take it out and adjust the pH to 6.5 with 1mol / L NaOH solution, then centrifuge at 3000rpm for 15min; remove the supernatant, dry the precipitate at 70°C, and grind it through a 1mm sieve.

[0041] The cell density per unit volume of the immobilized bacterial particles prepared by the above steps is very high, 3.16×10 11 individual / g, which was 3 orders of magnitude higher than that of the control.

[0042] The immobilized particles remediate the soil polluted by polycyclic aromatic hydrocarbons according to the ratio of 2% of the particles, and the removal rate of PYR is 47% and the removal rate of BaP is 33% after 60 days of remediation.

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Abstract

Production of fixed bacterium as peat as carrier is carried out by alkalizing while moistening for peat, taking it as carrier material, steam sterilizing at high-temperature and high-pressure, inducing into bacterial suspension, multiplication culturing to obtain the final product. It's simple and efficient, has better growth environment, degradation and binding functions and excellent affinity ability.

Description

technical field [0001] The invention relates to cell immobilization technology, in particular to a method for preparing immobilized microbial particles for in-situ restoration of polycyclic aromatic hydrocarbon contaminated soil, mainly using peat soil as a carrier for adsorption and immobilization of microbial technology. Background technique [0002] Peat is partially fossilized plant residue, often dark brown in color. The composition of peat is complex, mainly lignin and cellulose. Peat has a porous structure, partially decomposed peat has almost 95% porous structure and a value of 200m 2 / g specific surface area, is a natural carrier material. [0003] Polycyclic aromatic hydrocarbons (PAHs) refer to a class of compounds with two or more benzene rings fused together, and they are ubiquitous in the environment. Human activities, especially the burning of fossil fuels, are the main source of PAHs in the environment. Due to the high octanol-water partition coefficient ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14
Inventor 李培军苏丹鞠京丽张海荣许华夏
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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