Enzyme membrane reactor and method of using enzyme membrane reactor to treat wastewater

An enzyme membrane reactor and waste water technology, applied in chemical instruments and methods, biological treatment devices, biological water/sewage treatment, etc., to achieve the effects of high reactivity, long service life and wide application range

Inactive Publication Date: 2018-12-07
中节能工程技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are few studies on the combination of enzyme polymerization and membrane filtration. The core difficulty of this research direction is how to maintain better enzyme activity and membrane flux, while achieving the stability and longer service life of the immobilized enzyme membrane.

Method used

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  • Enzyme membrane reactor and method of using enzyme membrane reactor to treat wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Preparation of immobilized enzyme membrane:

[0033] First prepare 0.25g / L sodium alginate aqueous solution, 2000u / ml horseradish peroxidase phosphate buffer, and the phosphate concentration is 10mM. Take 1.5mL of the prepared sodium alginate solution and horseradish peroxidase solution and mix evenly, and then coat the mixed solution on both sides of the nylon diaphragm (the diameter of the diaphragm is Φ100mm, and the aperture is 0.22μm), and let it stand 10min, then put into 0.1mol / L calcium chloride solution and let stand for 20min. Finally, stand in deionized water for 2.5 h before use.

[0034] 2) Enzyme membrane reactor to treat low-concentration phenol wastewater:

[0035] Treatment equipment composed of enzyme membrane reactors such as figure 1 , including an enzyme membrane reactor 3, a peristaltic pump 2, a beaker 1 containing 100mL of waste water, a water inlet pipe, a return water pipeline S1, and a final water outlet pipeline S2; the beaker 1 is conn...

Embodiment 2

[0040] 1) Preparation of immobilized enzyme membrane:

[0041] First prepare 0.5g / L sodium alginate aqueous solution, 3000u / ml horseradish peroxidase phosphate buffer, and the phosphate concentration is 10mM. Mix 1.5ml each of the prepared sodium alginate solution and horseradish peroxidase solution evenly, and then coat the mixed solution on a nylon diaphragm (Φ100mm, pore size 0.22μm), let it stand for 10min, and then put it in the Stand in 0.1mol / l calcium chloride solution for 20min. Finally, stand in deionized water for 2.5 h before use.

[0042] 2) Enzyme membrane reactor degrades bisphenol A, including:

[0043] The equipment structure for treating wastewater is the same as in Example 1, and the enzyme membrane reactor is made in step 1) of this example.

[0044] The immobilized enzyme membrane is placed in the reactor, and the peristaltic pump is started to flow the bisphenol A through the reactor, so that the bisphenol A fully reacts with the enzyme. The bisphenol...

Embodiment 3

[0047] 1) Preparation of immobilized enzyme membrane:

[0048]First prepare 0.125g / L sodium alginate aqueous solution, 1000u / ml horseradish peroxidase phosphate buffer, and the phosphate concentration is 10mM. Take 1.5ml of the prepared sodium alginate solution and horseradish peroxidase solution and mix evenly, then coat the mixed solution on a nylon diaphragm (Φ100mm, pore size 0.22μm), let it stand for 10min, and then put it in the Stand in 0.1mol / l calcium chloride solution for 20min. Finally, stand in deionized water for 2.5 h before use.

[0049] 2) Enzyme membrane reactor degrades rhodamine B, including:

[0050] The equipment structure for treating wastewater is the same as in Example 1, and the enzyme membrane reactor is made in step 1) of this example.

[0051] The immobilized enzyme membrane is placed in the reactor, and the peristaltic pump is started to flow the rhodamine B through the reactor, so that the rhodamine B fully reacts with the enzyme. The rhodamin...

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Abstract

The invention provides an enzyme membrane reactor. The enzyme membrane reactor comprises a microfiltration membrane and an immobilized enzyme, wherein the immobilized enzyme comprises a horseradish peroxidase which is fixed to the microfiltration membrane through an embedding medium. The invention also provides a method of using the enzyme membrane reactor to treat wastewater. The enzyme membranereactor and method have the advantages that the enzyme membrane reactor has high reaction activity and fast reaction speed and can basically complete reaction in ten minutes; reaction conditions are mild, and the reactor operates at a normal temperature and a normal pressure; an enzyme immobilization method is simple, and the production cost is low; an enzyme membrane has good stability, an enzymeis unlikely to run off, the membrane is unlikely to be blocked, and the service life is long; the enzyme membrane reactor can treat various pollutants such as phenols and azo, and has a wide application range; only a small amount of hydrogen peroxide is added, and the treatment cost is low; and no poisonous and harmful chemical agents are added, and thus, the secondary pollution of environments is avoided.

Description

technical field [0001] The invention belongs to the field of waste water treatment, and in particular relates to a treatment device and a treatment method for waste water containing organic pollutants. Background technique [0002] With the rapid development of industry, various kinds of refractory wastewater are increasing day by day. In particular, a large amount of industrial waste water discharged by various industries has become increasingly serious to the environment. Phenols, anilines, dyes and other wastewater are toxic pollutants with a wide range of industrial wastewater, causing serious harm to humans, water bodies, fish and crops. In recent years, researchers have developed various treatment methods. Currently, commonly used treatment methods such as reverse osmosis, liquid membrane, ultrafiltration, neutralization, precipitation, oxidation, reduction, electrolysis, microbial treatment, etc., However, these methods cannot achieve ideal results in the degradatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/30
CPCC02F3/342C02F2101/30C02F2203/004C02F2203/006C02F2301/046
Inventor 李海涛李英英刘杰杨忆新徐伟
Owner 中节能工程技术研究院有限公司
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