A method for preparing xanthus sorbifolium polypeptide based on papaya seed gum immobilized enzyme

A papaya seed gum, immobilized enzyme technology, applied in the directions of immobilization on/in organic carriers, hydrolase, fermentation, etc., can solve the problems of high immobilization cost, few immobilized enzymes, poor stability, etc., and achieves immobilization. Simple chemical method, good stability and good mechanical properties

Active Publication Date: 2020-09-08
陕西嘉康源健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the development of immobilized enzyme technology and the continuous expansion of application scope, immobilized enzyme has become one of the active research fields, and there are more and more studies on the immobilization of enzyme at home and abroad, but the real industrial application There are few immobilized enzymes, the main reasons are high immobilization cost, low efficiency and poor stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Dissolve 1g of papain, 3g of papaya seed gum and 3.8g of chromium acetate in phosphate buffer solution of 100g of pH=6.5 respectively to prepare papain solution with a mass concentration of 1% and papaya seed gum with a mass concentration of 3%. solution, a mass concentration of 3.8% chromium acetate solution; after mixing 20mL mass concentration of 3% papaya seed gum solution with 10mL mass concentration of 1% papain solution, add 60mL mass concentration of 3.8% chromium acetate solution , standing at 40° C. for 6 hours, and then centrifuging at 5000 rpm, and freeze-drying the precipitate to obtain papaya seed gum-immobilized enzyme.

[0017] 2. After adjusting the pH value to 6.5 with phosphate in the 2% aqueous solution of radix sorbifolia protein, add papaya seed gum to immobilize the enzyme according to the ratio of 3300U papain / g sorbifolia protein, and enzyme at 55°C Decompose for 60 minutes, after enzymolysis finishes, carry out centrifugation at a centrifuga...

Embodiment 2

[0019] 1. Dissolve 1.5g of protease (trypsin and neutral protease in an activity ratio of 1:1 mixture), 2.5g of papaya seed gum, and 3g of chromium propionate in 100g of phosphate buffer at pH=8.0, and prepare the mass concentration 1.5% protease solution, 2.5% papaya seed gum solution, 3% chromium propionate solution; 20mL 2.5% papaya seed gum solution and 1.5% protease After the solution was mixed evenly, 40 mL of chromium propionate solution with a mass concentration of 3% was added, and it was left to stand at 50° C. for 3 hours, then centrifuged at a speed of 5000 rpm, and the precipitate was freeze-dried to obtain papaya seed gum-immobilized enzyme.

[0020] 2. Adjust the pH value of the 4.5% sorbifolia protein aqueous solution to 8.0 with phosphate, add papaya seed gum to immobilize the enzyme according to the ratio of 3300U protease / g sorbifolia protein, and enzymatically hydrolyze at 50°C 180 minutes, after enzymatic hydrolysis, centrifugal separation was performed at...

Embodiment 3

[0022] 1, 2.5g alkaline protease, 4g papaya seed gum, 2.5g chromium acetate are dissolved in the phosphate buffer solution of 100g pH=9.0 respectively, are mixed with the alkaline protease solution that mass concentration is 2.5%, the mass concentration is 4% Papaya seed gum solution, a mass concentration of 2.5% chromium acetate solution; after mixing 20mL of a mass concentration of 4% papaya seed gum solution with 10mL of a mass concentration of 2.5% alkaline protease solution, adding 24mL of a mass concentration of 2.5% The chromium acetate solution was allowed to stand at 45° C. for 2.5 hours, then centrifuged at a speed of 5000 rpm, and the precipitate was freeze-dried to obtain papaya seed gum-immobilized enzyme.

[0023] 2. After adjusting the pH value to 9.0 with phosphate in the aqueous solution of radix sorbifolia protein with a mass concentration of 3%, add papaya seed gum to immobilize the enzyme according to the ratio of 4000U alkaline protease / g radix sorbifolia p...

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PUM

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Abstract

The invention discloses a method for preparing a Xanthoceras sorbifolia polypeptide based on a papaya seed gum immobilized enzyme. The method uses papaya seed gum as a carrier and a trivalent chromium salt as a cross-linking agent to immobilize the biological enzyme. The immobilization method is simple and has high efficiency. The immobilized enzyme is physical gel with good stability, and the immobilized enzyme is free of a costly carrier so that a cost is greatly reduced. The immobilized enzyme can realize enzymolysis of a Xanthoceras sorbifolia protein to extract the polypeptide. The method has simple processes, is easy to operate, realizes enzyme recovery and recycling and greatly reduces the waste of resources.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a method for preparing an immobilized enzyme using papaya seed gum as a carrier to enzymolyze radix radix radix radix protein. Background technique [0002] Xanthoceras sorbifolia Bunge is a deciduous tree or large shrub of the genus Xanthoceras sorbifolia. It is native to the Loess Plateau and is now widely distributed in northern my country. Widely planted in barren hilly areas. It is a rare woody oil tree species unique to China, and it is also a promising soil and water conservation tree species and rare ornamental tree species in northern China. As my country is the country of origin of X. sorbifolium, the economic benefits and application prospects of X. sorbifolium resource development are immeasurable. The seeds of X. sorbifolium are not only edible, but also can be developed into health care products. The kernels of X. sorbifolium are rich in oil and protei...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12N11/02
CPCC12N9/50C12N11/02C12P21/06
Inventor 金昱辰谷飞云
Owner 陕西嘉康源健康产业有限公司
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