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A kind of preparation method and application of immobilized lactic acid bacteria

A lactic acid bacteria, sericin technology, applied in biochemical equipment and methods, microorganism-based methods, immobilized on/in organic carriers, etc., can solve the problem of poor anti-microbial decomposition performance, low mechanical strength, and softening and other problems, to achieve the effect of not easy to contaminate bacteria, excellent mechanical properties, and less interfering substances

Active Publication Date: 2020-11-13
浙江益弘食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium alginate is used in these methods, but its antimicrobial decomposition performance is poor, and its mechanical strength is low. At the same time, sodium alginate will become soft and sticky under the repeated impact of high-concentration phosphate buffer.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) preparing immobilized lactic acid bacteria, comprising the steps of:

[0037] S1. Prepare lactic acid bacteria concentrate: centrifuge and concentrate the lactic acid bacteria suspension after enrichment culture to obtain a bacterial count of 1×10 8 CFU / ml lactic acid bacteria concentrate;

[0038] S2, lactic acid bacteria immobilization:

[0039] Dissolve sericin in ultrapure water to obtain an aqueous sericin solution with a concentration of 2% by mass, and mix the sericin aqueous solution with the lactic acid bacteria concentrate prepared in step S1 to obtain liquid A; drop into the obtained liquid A into the cross-linking agent, fully mixed and cross-linked at 36°C for 8 hours to obtain a hydrogel containing lactic acid bacteria; dissolve the cellulose powder in water to obtain a cellulose solution with a mass percentage concentration of 1% swelling, and add it to the water Gel, after mixing, cross-link for 5 hours under stirring at 36°C; slowly drop the obtain...

Embodiment 2

[0043] 1) preparing immobilized lactic acid bacteria, comprising the steps of:

[0044] S1. Prepare lactic acid bacteria concentrate: centrifuge and concentrate the lactic acid bacteria suspension after enrichment culture to obtain a bacterial count of 1×10 8 CFU / ml lactic acid bacteria concentrate;

[0045] S2, lactic acid bacteria immobilization:

[0046] Dissolve sericin in ultrapure water to obtain an aqueous sericin solution with a concentration of 3% by mass, and mix the sericin aqueous solution with the lactic acid bacteria concentrate prepared in step S1 to obtain liquid A; drop into the obtained liquid A into the cross-linking agent, fully mixed and cross-linked at 34°C for 5 hours to obtain a hydrogel containing lactic acid bacteria; dissolve the cellulose powder in water to obtain a swollen cellulose solution with a concentration of 2% by mass, and add it to the water Gel, after mixing, cross-link for 10 hours under stirring at 36°C; slowly drop the obtained cross...

Embodiment 3

[0049] Embodiment 3 comparative experiment

[0050] 1) preparing immobilized lactic acid bacteria, comprising the steps of:

[0051] S1. Prepare lactic acid bacteria concentrate: centrifuge and concentrate the lactic acid bacteria suspension after enrichment culture to obtain a bacterial count of 1×10 8 CFU / ml lactic acid bacteria concentrate;

[0052] S2, lactic acid bacteria immobilization:

[0053] Dissolve sericin in ultrapure water to obtain an aqueous sericin solution with a concentration of 1% by mass, and mix the sericin aqueous solution with the lactic acid bacteria concentrate prepared in step S1 to obtain liquid A; drop into the obtained liquid A into the cross-linking agent, fully mixed and cross-linked at 30°C for 2 hours to obtain a hydrogel containing lactic acid bacteria; dissolve the cellulose powder in water to obtain a cellulose solution with a mass percentage concentration of 3% swelling, and add it to the water Gel, mix well and cross-link for 20 hours ...

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PUM

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Abstract

The invention discloses a preparation method and an application of an immobilized lactobacillus. The preparation method includes the steps: S1 lactobacillus concentrated solution preparation: centrifugally concentrating lactobacillus suspension after enrichment culture to obtain lactobacillus concentrated solution; S2 lactobacillus immobilization: 1) swelling sericin protein by water to obtain sericin protein solution, uniformly mixing the sericin protein solution and the lactobacillus concentrated solution to obtain liquid A; 2) dripping a cross-linking agent into the liquid A acquired in thestep 1) to obtain sericin protein hydrogel containing lactobacilli; 3) dissolving cellulose powder in water to obtain swelling cellulose solution, adding the swelled cellulose solution into the sericin protein hydrogel acquired in the step 2), uniformly mixing mixture, and crosslinking the mixture; 4) slowly dripping calcium chloride solution into crosslinked solution acquired in the step 3) through a constant-flow pump, filtering mixture, washing the mixture by deionized water to obtain the immobilized lactobacillus, and storing the immobilized lactobacillus in a refrigerator with the temperature of 4 DEG C. The immobilized lactobacillus is used for preparing gamma-aminobutyric acid in a fermented manner.

Description

technical field [0001] The invention belongs to the technical field of preparation of immobilized lactic acid bacteria, and in particular relates to a preparation method and application of immobilized lactic acid bacteria. Background technique [0002] γ-Aminobutyric acid (GABA), also known as aminobutyric acid, is a white crystalline powder, non-protein amino acid widely distributed in animals and plants, and is the main inhibitory neurotransmitter of the central nervous system. It has an amino group at the γ-position of butyric acid, exists in an unbound state, is easily soluble in water, slightly soluble in hot ethanol, not easily soluble in alcohol, ether and phenol, has a sweetness similar to glutamic acid, and can Enhance the flavor of food. Its molecular formula is C 4 h 9 NO 2 , the molecular weight is 103.12. [0003] GABA can inhibit the excessive excitement of the central nervous system, have a stabilizing effect on the brain, and then promote relaxation and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/12C12N11/10C12N11/02C12P13/00C12R1/01
CPCC12N11/02C12N11/10C12N11/12C12P13/005
Inventor 刘士旺方理明龚金炎肖功年楼坚柳永鲍文娜楚秉泉张亚青
Owner 浙江益弘食品有限公司
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