Fungus and bacterium combined immobilizing method using roseite as carrier

A technology of vermiculite and fungus, applied in the field of cell immobilization, to achieve the effects of increased resistance, obvious repair effect, and improved immobilization efficiency

Inactive Publication Date: 2010-07-21
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports at home and abroad on the use of immobilized microorganisms for the degradation of organic pollutants in non-fluid media (soil).

Method used

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  • Fungus and bacterium combined immobilizing method using roseite as carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Incline culture of immobilized mixed bacteria

[0032] In the culture medium (glucose 1.25%, beef extract 0.25%, NH 4 NO 3 0.1%, MgSO 4 ·7H 2 O 0.02%, KCl 0.02%, pH 6.5) inoculated bacteria and fungi successively in a test tube, placed in an incubator at 28-30°C for 2-3 days.

[0033] (2) Immobilized carrier modification treatment

[0034] The vermiculite needs to be modified before it is used as a carrier. The specific operation steps are: pass the vermiculite through a 2mm sieve, add 1mol / L HCl, the solid-liquid ratio is 1:20, soak at room temperature for 24 hours, and wash with tap water until the pH value The pH value of the tap water (about 6.8), then dried at 60°C, and placed in a desiccator for later use, then acid-modified vermiculite at room temperature can be prepared.

[0035] (3) Immobilized carrier infiltration culture

[0036] The modified vermiculite is infiltrated with sterile water at a ratio of 1ml / g for 12-24 hours.

[0037] (4) Preparatio...

Embodiment 2

[0043] The difference from Example 1 is that the carrier modification treatment step is to add 0.5mol / L HCl to vermiculite with a particle size of 2mm, the solid-to-liquid ratio is 1:15, stir in a constant temperature water bath at 80°C for 2-2.5h, and rinse with distilled water to pH It is 6.8, and after drying, the heated acid-modified vermiculite is obtained.

[0044] The cell density per unit volume of the immobilized bacterial particles obtained through the above steps is 7.21×10 8 individual / g, the number of fungal spores is 5.38×10 6 pcs / g.

[0045] The immobilized particles remediate the soil polluted by polycyclic aromatic hydrocarbons according to the ratio of 2% to 2%, and the removal rate of PYR is 81% and the removal rate of BaP is 59% after 60 days of remediation.

Embodiment 3

[0047] The difference is that the carrier modification treatment step is to add 1mol / L HCl to vermiculite with a particle size of 2mm, the solid-to-liquid ratio is 1:20, stir in a constant temperature water bath at 83°C for 2-2.5h, rinse with distilled water until the pH is 6.8, and dry After that, the heated acid-modified vermiculite is obtained.

[0048] The cell density per unit volume of the immobilized bacterial particles prepared by the above steps is relatively large, 1.59×10 9 individual / g, the number of fungal spores is 2.42×10 7 pcs / g.

[0049] The immobilized particles remediate the soil polluted by polycyclic aromatic hydrocarbons according to the ratio of 2% of the particles, and the removal rate of PYR is 85% and the removal rate of BaP is 59% after 60 days of remediation.

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Abstract

Technology for fixing fungi and bacterium with vermiculite as carrier is carried out by modifying while moistening for vermiculite as carrier material, steam sterilizing at high-temperature and high-pressure, inoculating mixed bacterium, and multiplication culturing to obtain the final product. It's simple, porous and efficient, has better growth environment, degradation and resistance to poisonous, larger specific area and competition ability.

Description

technical field [0001] The invention relates to cell immobilization technology, in particular to a method for preparing immobilized particles for repairing polycyclic aromatic hydrocarbon contaminated soil, and is an adsorption and immobilization mixed bacteria process using vermiculite as a carrier. Background technique [0002] The chemical formula of vermiculite is Mg x (H 2 O){Mg 3～x [AlSiO 3 o 10 〕(OH) 2}, is a layered magnesium-containing hydroaluminosilicate secondary metamorphic mineral, which looks like mica, and is usually formed by hydrothermal alteration or weathering of black (golden) mica. It is called vermiculite because it is deflected when it loses water and expands when heated, and its shape resembles a leech. Vermiculite has excellent properties such as heat insulation, freezing resistance, and water absorption, and is an excellent carrier material. [0003] Polycyclic aromatic hydrocarbons (PAHs) refer to a class of compounds with two or more benze...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N1/14C12N1/20
Inventor 苏丹李培军鞠京丽尹炜
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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