Integrated solid state fermentation reactor and application thereof

A solid-state fermentation and reactor technology, applied in the field of integrated solid-state fermentation reactors, can solve the problems of restricting the rapid development of solid-state fermentation technology, lack of mass transfer and heat dissipation, and narrow application scope, and achieves remarkable drying effect, uniform heating, and avoidance of impurities. The effect of bacterial contamination

Inactive Publication Date: 2015-08-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the existing solid-state fermentation reactors lack relatively comprehensive research on in-situ sterilization, automatic control, mass transfer and heat dissipation, an

Method used

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  • Integrated solid state fermentation reactor and application thereof
  • Integrated solid state fermentation reactor and application thereof
  • Integrated solid state fermentation reactor and application thereof

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Experimental program
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Effect test

Embodiment 1

[0047] like figure 1 The solid-state fermentation belt reactor shown includes a fermenter 1, a medium preparation tank 10 and a conveyor belt system 11; wherein the fermenter 1 is a stainless steel horizontal fermenter, and the first support 3-1 at the bottom and the second The support 3-2 is fixed on the base 3. A jacket 4-1 is provided on the outer periphery of the fermenter, and the jacket 4-1 is provided with a heat exchange medium inlet 4-2 and a heat exchange medium outlet 4-3, and the heat exchange medium inlet 4-2 is arranged on the jacket 4 -1 is at the top, the heat exchange medium outlet 4-3 is located at the bottom of the jacket 4-1, and the heat exchange medium inlet 4-2 and the heat exchange medium outlet 4-3 are far apart in the horizontal direction. The heat exchange medium is water vapor and circulating cooling water.

[0048]A first movable top cover 2-1 and a second movable top cover 2-2 are arranged on both sides of the fermentation tank 1 in the horizont...

Embodiment 2

[0054] Embodiment 2: Aerobic microbial solid-state fermentation (taking Bacillus subtilis solid-state fermentation to produce gamma-polyglutamic acid agricultural fertilizer as an example)

[0055] γ-polyglutamic acid (γ-PGA) is a kind of homopolyamino acid synthesized by microorganisms. The basic skeleton is formed by linking L-glutamic acid and D-glutamic acid through γ-amide bonds. straight-chain molecules, with a relative molecular mass of up to 200×10 4 Da. Due to the existence of a large number of hydrogen bonds and hydrophilic free carboxyl groups in its structure, it has excellent moisture retention properties and has been widely used in agricultural fertilizers.

[0056] The microorganism fermented in the present embodiment is the Bacillus subtilis NX-2 ((bacteria strain preservation number: CGMCC 0833) that this laboratory has obtained authorization, directly uses microbial bacterial agent (5 * 10 10 cfu / g) inoculation, the inoculation amount is 25g / kg.

[0057] S...

Embodiment 3

[0061] Embodiment 3: aerobic microorganisms take solid-state fermentation of Bacillus subtilis phosphate-solubilizing bacteria bacterial agent as an example

[0062] Phosphorus is one of the essential nutrients for plant growth and development. Soil phosphorus deficiency directly affects the growth of crops and food production. In my country, the area of ​​phosphorus-deficient cultivated land reaches 74%, and more than 95% of the phosphorus in the soil is in an invalid state, which is difficult to be absorbed. Plants directly absorb and utilize. There are many phosphorus-solubilizing microorganisms in the soil, which can degrade these insoluble phosphates into free forms for plant absorption and utilization. Therefore, it is necessary to develop phosphorus-solubilizing microorganisms, reduce the production cost of phosphate fertilizers, solve the pollution of phosphate fertilizers and improve the utilization rate of phosphorus. Research is necessary.

[0063] The phosphate-sol...

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Abstract

The invention discloses an integrated solid state fermentation reactor. The reactor comprises, sequentially connected, a charging system, a fermentation tank and a rotary vacuum double-cone drying machine; and the charging system comprises, sequentially connected, an electric motor, a charging hopper, a conveying screw and a discharging tube, a stainless steel jacket is welded on the outer wall of the body of the fermentation tank, and the fermentation tank is also provided with a gas outlet, probes, a steam inlet, a steam outlet, a discharge opening, movable top covers and a two-fluid nozzle device. The invention also discloses an application of the reactor in solid state fermentation of aerobic, anaerobic and facultative anaerobic microbes. Compared with reactors in the prior art, the reactor has the advantages of high automation intensity, stable fermentation process, simple operation, realization of single-batch and multi-batch fermentation production, and facilitation of industrial application of solid state fermentation.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering and process engineering, and in particular relates to an integrated solid-state fermentation reactor and its application. Background technique [0002] Biotechnology is the most influential high-tech emerging industry belt in this century and the most vital economic growth chain. Bioengineering will become a pillar industry for my country's key development in the 21st century. The development of biotechnology will have great impact on various fields of human life. growing influence. [0003] Solid-state fermentation is a biological reaction process carried out by microorganisms using solid substrates as energy sources and carriers. Due to the large gas-solid surface area of ​​the medium used, microorganisms are easy to grow, and because microorganisms grow in the closest natural state, they can produce some enzymes or other metabolites that will not be produced during liquid ferme...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/16C12M1/12C12M1/04C12M1/02C12M1/00
Inventor 冯小海王程梁金丰汤宝徐虹许宗奇
Owner NANJING UNIV OF TECH
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