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Method for preparing amino acid liquid water-soluble fertilizer through compound stepped fermentation of seaweed

An amino acid and water-soluble fertilizer technology, applied in the direction of microorganism-based methods, liquid fertilizers, biochemical equipment and methods, etc., can solve the problems of soil compaction and acidification, inability to form population effects, and inability to exert the effect of microbial agents, so as to promote plant growth. Root growth, remarkable product efficacy, stable and controllable fermentation process

Inactive Publication Date: 2017-01-25
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the severe compaction and acidification of the soil and the erratic changes in the outside temperature, the microbial agents cultivated in the greenhouse are directly put into the soil, causing a large number of deaths because they cannot adapt to the harsh soil environment. The effect of the agent

Method used

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  • Method for preparing amino acid liquid water-soluble fertilizer through compound stepped fermentation of seaweed
  • Method for preparing amino acid liquid water-soluble fertilizer through compound stepped fermentation of seaweed
  • Method for preparing amino acid liquid water-soluble fertilizer through compound stepped fermentation of seaweed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Grind the washed and dried fresh sargassum into 50-60 mesh powder.

[0042] Seaweed powder and water were loaded into a fermenter at a mass ratio of 4:100, and sterilized at 115°C for 25 minutes to obtain product A.

[0043] And prepare the seed solution:

preparation example

[0044] Example of preparation of seed liquid X: Inoculate cellulase-producing Bacillus megaterium YIC~BM1 into liquid medium, the volume ratio of Bacillus megaterium YIC~BM1 to the medium is 5:100, the fermentation temperature is 32°C, and acid Alkali regulator adjusts the pH value of the reaction to 6.5, the dissolved oxygen is controlled at 20%, the rotation speed is 200r / min, the ventilation rate is 800L / min, the pressure of the fermenter should be maintained at 0.03Mpa, and the fermentation time is 20h; after activation, follow the same operation The step is to expand the cultivation to obtain the seed liquid X;

[0045] Preparation example of seed liquid Y: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium, the volume ratio of Bacillus saccharophilus YIC~Alg3 to medium is 5:100, the fermentation temperature is 30°C, and acid-base regulator is used Adjust the pH value of the reaction to 7.2, control the dissolved oxygen at 20%, the rotation speed at 200r / min, t...

Embodiment 2

[0048] Grinding the washed and dried fresh copper algae into 50-60 mesh powder;

[0049] Seaweed powder and water were loaded into a fermenter at a mass ratio of 4:100, and sterilized at 115°C for 25 minutes to obtain product A.

[0050] And prepare the seed solution:

[0051] Example of preparation of seed liquid X: Inoculate cellulase-producing Bacillus megaterium YIC~BM1 into liquid medium, the volume ratio of Bacillus megaterium YIC~BM1 to the medium is 5:100, the fermentation temperature is 32°C, and acid Alkali regulator adjusts the pH value of the reaction to 6.5, the dissolved oxygen is controlled at 20%, the rotation speed is 200r / min, the ventilation rate is 800L / min, the pressure of the fermenter should be maintained at 0.03Mpa, and the fermentation time is 24h; after activation, follow the same operation The step is to expand the cultivation to obtain the seed liquid X;

[0052] Preparation example of seed liquid Y: inoculate Bacillus saccharophilus YIC~Alg3 into...

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Abstract

The invention relates to a method for preparing an amino acid liquid water-soluble fertilizer through compound stepped fermentation of seaweed. The method comprises the following steps: grinding the dried seaweed into powder, filling in a fermentation tank and sterilizing; respectively preparing seed solutions of two strains, separately inoculating the seed solutions in bottom material of seaweed to realize stepped fermentation so as to produce a majority of small molecule specific metabolites such as seaweed oligosaccharide which have a promotion effect on growth of crops to improve biological activity of seaweed fertilizers; carrying out pressure filtration to obtain seaweed solutions after the fermentation is finished; adding the seaweed solutions and amino acid containing organic matters in a stirring kettle according to proportions; stirring and completely dissolving to obtain the amino acid liquid water-soluble fertilizer containing the seaweed solutions. The effect of the compound stepped fermentation technology adopted by the invention is obviously better than that of single strain fermentation and common multiple-strain mixed fermentation; moreover, the fermentation efficiency is high, the time is short and the whole technological maneuverability is strong. The product of the invention combines amino acid with effective components of seaweed, and has an obvious effect on stress resistance and disease resistance.

Description

technical field [0001] The invention relates to a production method of amino acid liquid water-soluble fertilizer. Background technique [0002] Seaweed fertilizer is a new type of organic fertilizer that uses natural seaweed as raw material, undergoes special biochemical processing, extracts and retains the natural active ingredients of seaweed, refines and concentrates. Seaweed fertilizer has the effect of improving crop yield and quality, resisting pests and diseases and adverse environment, and improving soil and water conservation. Its application in agricultural production has huge market demand and good development prospects. However, in order to ensure the quality of seaweed fertilizer, two key links of seaweed raw material and extraction process must be grasped. [0003] First of all, the quality of seaweed fertilizer depends on the raw materials used. At present, the raw materials used by seaweed fertilizer manufacturers are mainly brown algae, including Ascophyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C12N1/20C12R1/11C05G3/80
CPCC05C11/00C05G3/00C12N1/20C05G3/80C05G5/20C05F11/00C05F11/08
Inventor 王学江袁蕊
Owner WUZHOUFENG AGRI SCI & TECH
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