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Green and clean fermentation technology of glutamic acid

A fermentation process, glutamic acid technology, applied in the field of green and clean fermentation process of glutamic acid, can solve the problems of large differences in cell membrane structure and irregular rules to follow

Active Publication Date: 2019-04-19
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many substances to adjust the permeability of the cell membrane, and the structure of the cell membrane varies greatly among different strains, so there is no rule to follow. Choosing an appropriate method to adjust the permeability of the cell membrane is also a technology that needs to be solved in the glutamic acid fermentation process question

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The strain used in this experiment is Corynebacterium glutamicum GDK-9 (also known as Brevibacterium flavum GDK-9, which is derived from Tianjin University of Science and Technology. The source of the strain can also be found in "Research on variable temperature control process of L-glutamic acid fermentation, Tianjin Chemical Industry Co., Ltd. year 2010").

[0032] The green clean fermentation technology of glutamic acid, it comprises the steps: Corynebacterium glutamicum GDK-9 is pressed seed liquid (OD 600nm 10) Connect to a 50L fully automatic fermenter equipped with 30L clean fermentation medium for fermentation and cultivation, the fermentation temperature is 38°C, the ventilation ratio is 1:0.7, the stirring speed is 500r / min, the dissolved oxygen is maintained at 20%, and the feed mass Glucose with a percentage concentration of 80% maintains a residual sugar of 1.5%, adding a defoamer to defoam, and the total fermentation time is 33h; wherein, the fermentation ...

Embodiment 2

[0042] The green clean fermentation technology of glutamic acid, it comprises the steps: Corynebacterium glutamicum GDK-9 is pressed seed liquid (OD 600nm 14) Connect to a 50L fully automatic fermenter equipped with 30L clean fermentation medium for fermentation and cultivation, the fermentation temperature is 37°C, the ventilation ratio is 1:0.8, the stirring speed is 300-700r / min, and the dissolved oxygen is maintained at 25%. Add glucose with a mass percentage concentration of 80% to maintain the residual sugar at 1%, add defoaming agent to defoam, and the total fermentation time is 34h; among them, the fermentation culture is 0-8h, and a high-temperature resistant ultrasonic probe is inserted in the fermenter. Finally, turn on the ultrasonic controller and perform ultrasonic treatment under certain conditions. The conditions of ultrasonic treatment are: ultrasonic power 500W, frequency 20kHZ, ultrasonic time 50s, amplitude 65%, interval 5min, and adjust by adding 25% ammoni...

Embodiment 3

[0050] Optimization of glutamic acid fermentation medium.

[0051] 1. The effect of the addition amount of bacterial proteolysis liquid on the concentration of bacteria in the fermentation broth and the output of glutamic acid. The addition amount was selected as five concentration gradients of 20, 40, 60, 80, 100, and 120 (ml / L). It was found that with Bacteria in the fermentation broth increased with the addition amount

[0052] Table 1

[0053] Addition amountml / L

Cell OD in fermentation broth 600nm

Glutamic acid production g / L

20

60.7

143.6

40

68.9

152.1

60

75.3

159.8

80

81.8

167.0

100

80.1

163.5

120

76.9

156.9

[0054] Substitute corn steep liquor (10g / L, OD 600nm 65.8, glutamic acid output 151g / L, light transmittance (T 430 ) was 0.98), the data found that with the addition of the cell proteolysis solution, the cell concentration and glutamic acid content a...

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Abstract

The invention belongs to the technical field of amino acid production, and discloses a green and clean fermentation technology of glutamic acid. The method comprises the steps that corynebacterium glutamicum is inoculated into a fermentation tank containing a clean fermentation culture medium to perform fermentation culturing, and auxiliary ultrasonic treatment is conducted. The technology has theadvantages that the fermentation efficiency is improved, and the separation difficulty of the glutamic acid is reduced.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a green and clean fermentation process of glutamic acid. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid was discovered by Li Suxun in 1856. It is a colorless crystal with umami taste, slightly soluble in water, but soluble in hydrochloric acid solution, with an isoelectric point of 3.22. A large amount exists in cereal protein, and the content in animal brain is also more. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. Sodium glutamate, commonly known as monosodium glutamate, is an important umami agent that enhances the fragrance. Sodium glutamate is widely used as a food flavoring agent, either ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/18C12R1/15
CPCC12P13/18
Inventor 李德衡赵兰坤徐庆阳马延和孙际宾刘元涛许传高户红通郑平高翠娟赵凤良孙钦波范婷婷贾召鹏
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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