Orderly, directional and co-immobilization enzyme membrane reactor and preparation method and application thereof

An enzyme membrane reactor and a technology for immobilizing enzymes, which are applied in the field of reactors, can solve the problems of complex preparation methods and low conversion efficiency, and achieve the effects of improving transfer efficiency, mild reaction conditions and improving catalytic reaction efficiency.
CN110540938APending Publication Date: 2019-12-06ZHEJIANG SCI-TECH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG SCI-TECH UNIV
Publication Date
2019-12-06

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Abstract

The invention discloses an orderly, directional and co-immobilization enzyme membrane reactor and a preparation method and application thereof. On the basis of a pervaporation membrane assembly, the pervaporation membrane assembly comprises a pervaporation membrane composited with a microfiltration membrane, immobilized enzymes of combination enzymes needed for a reaction are sequentially immobilized to the microfiltration membrane according to the enzyme catalysis cascade reaction sequence, the combination enzymes are any one of combination of horse radish peroxidase and glucose oxidase, combination of tyrosinase and alkaline phosphatase, combination of long-chain alcohol oxidase and omega-transaminase, and combination of ethanol dehydrogenase, cyclohexanone monooxygenase and lipase, thecatalytic reaction efficiency is advantageously improved, the immobilized enzymes are integrated on one microfiltration membrane, and the multi-step catalytic intermediate product transferring efficiency is improved; by arranging the microfiltration membrane, reacting and separating are integrated, and reaction balance advantageously moves in the favorable direction; and reaction conditions are mild, and an enzyme immobilization method is simple.
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Description

technical field

[0001] The invention belongs to the technical field of reactors, and in particular relates to an ordered and oriented co-immobilized enzyme-membrane reactor as well as a preparation method and application thereof. Background technique

[0002] Enzymes act as biocatalysts that increase the rate of a broad and diverse range of chemical reactions that occur in living organisms. It participates in important chemical reaction processes necessary for life, such as DNA replication and transcription, protein synthesis, primary and secondary metabolism, signal transduction and cellular defense mechanisms, and is an integral part of living organisms. Enzymes are widely used in biocatalysis, biosensing, and biomedical devices. However, due to the low thermal stability, poor stability of organic solvents, high cost and poor reusability of enzymes, the wide application of enzymes has been greatly hindered.

[0003] Enzyme immobilization technology is one of the effectiv...

Claims

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