Preparation method and application of co-immobilization mycelium pellets with function of removing various pollutants

A technology for mycelium balls and pollutants, which is applied in the field of preparation of co-immobilized mycelium balls, and can solve the problems of single degradation function and poor degradation effect of mycelium balls.

Active Publication Date: 2017-01-04
HARBIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the problem that the degradation function of existing mycelium balls is relatively single and the degradation effect is not good, and provides a method for preparing a co-immobilized mycelium ball with the function of removing various pollutants and its application

Method used

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  • Preparation method and application of co-immobilization mycelium pellets with function of removing various pollutants
  • Preparation method and application of co-immobilization mycelium pellets with function of removing various pollutants
  • Preparation method and application of co-immobilization mycelium pellets with function of removing various pollutants

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specific Embodiment approach 1

[0031] Specific embodiment one: the preparation method of the co-immobilized mycelium ball with the function of removing various pollutants in this embodiment is carried out according to the following steps:

[0032] 1. Preparation of bacterial suspension:

[0033] Aspergillus fumigatus spore suspension: take out the solid slope of Aspergillus fumigatus preserved at 4°C, scrape it off with an inoculation loop, insert it into sterile water, and make a concentration of 1×10 6 pcs / mL~9×10 6 Individual / mL of Aspergillus fumigatus spore suspension;

[0034] Bacillus cereus bacterial suspension: take out the solid slope of Bacillus cereus preserved at 4°C, scrape it off with an inoculation loop, put it into sterile water, and make a concentration of 1×10 6 A / mL~5×10 6 Individual / mL suspension of Bacillus cereus;

[0035] Two, the preparation method of composite bacteria mycelium ball:

[0036] Take the Aspergillus fumigatus spore suspension prepared in step 1 and the Bacillus c...

specific Embodiment approach 2

[0039] Specific embodiment two: this embodiment is different from specific embodiment one: the concentration of Aspergillus fumigatus spore suspension in step one is 3 * 10 6 pcs / mL~7×10 6 individual / mL. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0040] Specific embodiment three: this embodiment is different from specific embodiment one: the concentration of Aspergillus fumigatus spore suspension in step one is 5 * 10 6 individual / mL. Others are the same as in the first embodiment.

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Abstract

The invention belongs to the field of wastewater treatment, discloses a preparation method and application of co-immobilization mycelium pellets with the function of removing various pollutants, and relates to a preparation method and application of co-immobilization mycelium pellets. The problems that an existing mycelium pellet degradation function is single and the degradation effect is poor are solved. The method comprises the steps of preparing an aspergillus fumigatus spore suspension and a bacillus cereus suspension; 2, taking the aspergillus fumigatus spore suspension and the bacillus cereus suspension, inoculating the aspergillus fumigatus spore suspension and the bacillus cereus suspension into a mycelium pellet pelletizing culture medium, performing shake cultivation, and obtaining the compound mycelium pellets. The co-immobilization mycelium pellets are used for degrading lignin, cellulose, hemicellulose, dyes and heavy metal ions. The compound mycelium pellets can treat paper waste, can also be used for treating dye wastewater and heavy metal ion wastewater, and has the good treatment effect in a system with high alkaline and high temperature.

Description

technical field [0001] The invention relates to a preparation method and application of co-immobilized mycelium balls. Background technique [0002] The pulp and paper industry separates about 1.4 billion tons of cellulose from plants every year, and produces sewage containing about 50 million tons of lignin. In lignocellulose, lignin and hemicellulose are covalently bonded together to form a natural barrier that prevents cellulase from contacting cellulose. The key to utilizing cellulose is to destroy its barrier function, and hemicellulose Lignin is easy to remove, so the degradation of lignin becomes the key. The discharge of papermaking wastewater in my country accounts for 19% of the total industrial wastewater discharge, while the discharge compliance rate of wastewater is only 92%. The papermaking industry has become one of the key sources of environmental pollution in my country's industry. Printing and dyeing wastewater is a kind of refractory industrial wastewate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/16C02F3/34C12R1/085C12R1/68C02F103/16C02F103/28C02F101/30
CPCC02F3/34C02F2101/308C02F2103/16C02F2103/28C12N11/16
Inventor 燕红国巍杜霞李琳
Owner HARBIN UNIV OF SCI & TECH
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