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Method for preparing zinc gluconate through co-immobilization of glucose oxidase and catalase

A technology of glucose oxidase and catalase, applied to biochemical equipment and methods, oxidoreductase, immobilized on/in organic carriers, etc., can solve high production costs, complex process routes, and decolorization of fermentation broth Difficulties and other problems, to achieve the effect of no three waste pollution, high purity, and reduced production costs

Inactive Publication Date: 2017-05-31
温县兴发生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The production of zinc gluconate by Aspergillus niger fermentation requires sterilization of sugary raw materials, which will make it difficult to decolorize the fermentation broth; the fermentation ingredients need to add a variety of nutrient salts and introduce impurities, which will increase the cost of purification
And gained zinc gluconate product purity is lower
Moreover, it is necessary to ferment calcium gluconate first, and then use calcium gluconate as an intermediate to produce zinc gluconate. The process route is complicated, and sulfuric acid and calcium gluconate need to be reacted to generate calcium sulfate and gluconic acid. The production cost is extremely high, and the adopted Additives are harmful to the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0020] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction, add zinc oxide during the reaction process, and stop the reaction when the reducing sugar content in the reaction solution decreases to 0;

[0021] (3) The above reaction solution is ion-exchanged, crystallized and dried to obtain zinc gluconate.

[0022] In the step (1), the conditions for the cross-linking and esterification reaction are as follows: the reaction time is 8 hours in total, of which 0-4 hours are at a temperature of 35°C; 4-8 hours are at a temperature of 4°C...

Embodiment 2

[0025] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0026] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction, add zinc oxide during the reaction process, and stop the reaction when the reducing sugar content in the reaction solution decreases to 0;

[0027] (3) The above reaction solution is ion-exchanged, crystallized and dried to obtain zinc gluconate.

[0028] In the step (1), the conditions for the cross-linking and esterification reaction are as follows: the reaction time is 8 hours in total, of which 0-4 hours, the temperature is 38 ° C, and 4-8 hours, the temperature is 6 ° C...

Embodiment 3

[0031] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0032] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction, add zinc oxide during the reaction process, and stop the reaction when the reducing sugar content in the reaction solution decreases to 0;

[0033] (3) The above reaction solution is ion-exchanged, crystallized and dried to obtain zinc gluconate.

[0034] In the step (1), the conditions for the cross-linking and esterification reactions are: the reaction time is 8 hours in total, of which 0-4 hours are at a temperature of 40°C, and 4-8 hours are at a temperature of 8°C; gluc...

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Abstract

The invention discloses a method for preparing zinc gluconate through co-immobilization of glucose oxidase and catalase. Glucose is used as a substrate, and glucolactone, chitosan and calcium alginate are adopted for co-immobilization of glucose oxidase and catalase so as to achieve catalytic oxidation of glucose, so that zinc gluconate is prepared. By the adoption of the technique, enzyme activity is improved remarkably, the number of transformation steps is reduced, the glucose conversion rate is increased greatly, energy consumption is reduced, and production cost is reduced.

Description

technical field [0001] The invention relates to a preparation method of zinc gluconate, in particular to a method for co-immobilizing glucose oxidase and catalase to prepare zinc gluconate. Background technique [0002] Zinc is one of the essential trace elements for the human body. It plays a variety of physiological functions for maintaining the normal metabolism of the human body. The average content in the human body is 2.3 grams, which is slightly lower than that of iron, silicon, and fluorine. When the intake increases or decreases, it will cause metabolic imbalance, and then lead to the occurrence of diseases. Zinc deficiency can lead to children's developmental delay, mental retardation, malnutrition, and anorexia. Zinc deficiency in pregnant women is prone to irritability, depression, depression, anxiety, depression and other mood swings. High zinc intake will also have adverse effects on the metabolism of blood lipids, lipoproteins and apolipoproteins. Zinc gluco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12P7/58
CPCC12N11/10C12N9/0006C12N9/0065C12P7/58C12Y101/03004C12Y111/01006
Inventor 吴丽安民安庆堂冯斌斌
Owner 温县兴发生物科技有限公司
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