Method for extracting collagen peptide from grass carp scales

Abstract

The invention discloses a method for extracting a collagen peptide from grass carp scales. The method comprises steps as follows: fish scale pretreatment, protease co-immobilization, enzymolysis, ultrafiltration and freeze drying. 1-ethyl-3-(3-methylamino)propyl carbodiimide is used for activating chitosan, chitosan is coupled with glutaraldehyde to form a lipase immobilized carrier, and the hydrolysis degree, the enzymatic hydrolysis speed and the end group changing type of protein of the grass carp scales are improved through coordinated coordinated enzymatic hydrolysis of soybean protease and bromelain. The method has the benefits as follows: the obtained collagen peptide has small molecular weight and high purity and can directly reach the corium layer of skin to make skin tight, fine and smooth. The collagen peptide has a better antioxidant function, has a high scavenging rate for free radicals including DPPH., super O2<-> and .OH and has a remarkable anti-aging effect. The collagen peptide has an unexpected anti-tumor effect and has an anti-tumor specific T lymphocyte immune response while enhancing the non-specific immunity in a body.

Description

technical field [0001] The invention relates to the technical field of collagen extraction, in particular to a method for extracting collagen peptides from grass carp scales. Background technique [0002] my country is a major producer of freshwater fish in the world, and the freshwater fish production accounts for more than 60% of the world's total production. In recent years, the freshwater fish processing industry has developed rapidly and has become a new growth point of freshwater fishery. About 40% of by-products are produced during the processing of freshwater fish, including fish scales, fish heads and fish oil, etc. These leftovers are usually treated as waste or pig food cheaply, resulting in serious waste of resources and environmental pollution. Among them, fish scales are rich in nutrients and rich in collagen. The current existing methods for extracting collagen from fish scales generally have the problems of low extraction rate, low purity of collagen, and r...

AI Technical Summary

Problems solved by technology

The current existing methods for extracting collagen from fish scales generally have the problems of low extraction rate, low purity of collagen, and residual fishy smell, etc.