Method for extracting collagen peptide from grass carp scales

A technology of collagen peptides and fish scales, applied in chemical instruments and methods, animal/human proteins, biochemical equipment and methods, etc., can solve the problems of residual fishy smell, low purity of collagen, low extraction rate, etc., to achieve Small molecular weight, anti-tumor specific T lymphocyte immune response, and high clearance rate

Inactive Publication Date: 2017-12-29
浦江县欧立生物技术有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The current existing methods for extracting collagen from fish scales generally have the

Method used

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Experimental program
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Effect test

Embodiment 1

[0019] The method for extracting collagen peptides from grass carp fish scales comprises the following steps:

[0020] 1) Fish scale pretreatment: soak fresh fish scales, dry, crush, screen, decalcify, remove fish silver, pigment substances and soluble impurities;

[0021] 2) Protease co-immobilization: Take dry chitosan powder, soak it overnight with 60-80mmol / L phosphate buffer solution with pH 6.0-6.8, then add 0.7-1.2% 1-ethyl- 3-(3-methylamino)propyl carbodiimide was activated for 0.5~0.9h, then chitosan powder 1~2 times the volume of glutaraldehyde coupling reaction was added for 1~1.8h, and distilled water was used to wash for 5~8 hours. The second time, add soybean protease solution and bromelain solution, stir slowly in ice bath for 0.5~1.5h, then place in refrigerator overnight, wash thoroughly with distilled water the next day, filter and dry to obtain chitosan-immobilized compound enzyme. The concentrations of the soybean protease solution and the bromelain soluti...

Embodiment 2

[0026] The most preferred method for extracting collagen peptides from grass carp fish scales comprises the following steps:

[0027] 1) Fish scale pretreatment: soak fresh fish scales, dry, crush, screen, decalcify, remove fish silver, pigment substances and soluble impurities;

[0028]2) Co-immobilization of protease: Take dry chitosan powder, soak it overnight with 70mmol / L phosphate buffer solution with pH 6.5, then add 1-ethyl-3-(3-methyl Amino) propyl carbodiimide activation for 0.7h, then add chitosan powder 1.5 times the volume of glutaraldehyde coupling reaction for 1.5h, wash with distilled water 6 times, add 0.5~1.0mg / mL soybean protease solution and 0.8~2.0mg / mL bromelain solution, stirred slowly in an ice bath for 1h, then placed in the refrigerator overnight, rinsed thoroughly with distilled water the next day, filtered and dried to obtain chitosan-immobilized complex enzyme. The addition amount of soybean protease solution and bromelain solution is 4 times of c...

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PUM

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Abstract

The invention discloses a method for extracting a collagen peptide from grass carp scales. The method comprises steps as follows: fish scale pretreatment, protease co-immobilization, enzymolysis, ultrafiltration and freeze drying. 1-ethyl-3-(3-methylamino)propyl carbodiimide is used for activating chitosan, chitosan is coupled with glutaraldehyde to form a lipase immobilized carrier, and the hydrolysis degree, the enzymatic hydrolysis speed and the end group changing type of protein of the grass carp scales are improved through coordinated coordinated enzymatic hydrolysis of soybean protease and bromelain. The method has the benefits as follows: the obtained collagen peptide has small molecular weight and high purity and can directly reach the corium layer of skin to make skin tight, fine and smooth. The collagen peptide has a better antioxidant function, has a high scavenging rate for free radicals including DPPH., super O2<-> and .OH and has a remarkable anti-aging effect. The collagen peptide has an unexpected anti-tumor effect and has an anti-tumor specific T lymphocyte immune response while enhancing the non-specific immunity in a body.

Description

technical field [0001] The invention relates to the technical field of collagen extraction, in particular to a method for extracting collagen peptides from grass carp scales. Background technique [0002] my country is a major producer of freshwater fish in the world, and the freshwater fish production accounts for more than 60% of the world's total production. In recent years, the freshwater fish processing industry has developed rapidly and has become a new growth point of freshwater fishery. About 40% of by-products are produced during the processing of freshwater fish, including fish scales, fish heads and fish oil, etc. These leftovers are usually treated as waste or pig food cheaply, resulting in serious waste of resources and environmental pollution. Among them, fish scales are rich in nutrients and rich in collagen. The current existing methods for extracting collagen from fish scales generally have the problems of low extraction rate, low purity of collagen, and r...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/34C07K1/14C07K1/36C12P21/06C12N11/10
CPCC07K14/78C12N9/20C12N9/641C12N11/10C12P21/06C12Y301/01003C12Y304/22004C12Y304/22031
Inventor 李冰
Owner 浦江县欧立生物技术有限公司
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