The invention relates to detachable and reusable hydrophobic or super-hydrophobic micro-fluidic organ chips, which are constructed by using a hydrophobic or super-hydrophobic surface with low criticalsurface tension. Specifically, a heart chip, a liver chip, a brain chip, a tumor chip, a kidney chip, an intestinal chip, a skin chip, a fat chip, a blood vessel chip, a uterus chip, an eye chip, a nose chip, a bone chip, a periodontal chip, a pancreas islet chip, a spleen chip, a placenta chip, a lung chip, a muscle chip, a throat chip, a bone marrow chip, a diabetes chip and a multi-organ chipare constructed. The organ chips constructed by the method are detachable and can be repeatedly used, so that the application cost of the micro-fluidic organ chips is greatly reduced.