Amino-acid oxidase supported catalyst used for oxidizing amino acid to prepare ketonic acid

A supported catalyst and amino acid technology, applied in the direction of organic compound/hydride/coordination complex catalysts, physical/chemical process catalysts, chemical instruments and methods, etc., can solve the problem of low yield of ketoacids, regeneration of amino acid oxidase catalysis Reduce activity and other problems, achieve the effect of enhanced surface activity, excellent surface adsorption activity, and enhanced lipophilicity

Inactive Publication Date: 2017-09-05
MUDANJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of using amino acid oxidase to catalyze the oxidation of amino acids to prepare ketoacids has the problems of low yield of ketoacids and a significant decrease in the catalytic activity of regenerated amino acid oxidases. Therefore, it is urgent to develop new types of amino acid oxidations with strong catalytic activity and good regenerative properties to prepare ketoacids. biological enzyme catalyst

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of co-fixative: add 0.5 g of microcrystalline cellulose and 0.05 g of ultrafine magnesium oxide to 1.5 g of poloxamer, and heat up to 115-120 ° C at a heating rate of 5 ° C / min and mix for 15 min , then add 0.3g cationic polyacrylamide and 0.05g sodium alginate, continue to mix at 115-120°C for 15 minutes, transfer the resulting mixture to an environment of 0-5°C and keep it sealed for 1h, and heat up again at a rate of 5°C / min Speed ​​up to 115-120°C and keep mixing for 15 minutes, then add 10g of 60-65°C water, mix well, keep it at 60-65°C for 30 minutes, filter, and send the obtained filter residue into a spray dryer, and dry the obtained particles through ultra- Micro pulverizer into fine powder, that is, the co-fixative agent;

[0031] (2) Preparation of composite carrier: add 2.5g of ceramic micropowder, 0.3g of polyaspartic acid and 0.05g of sodium citrate to 10g of water, heat up to a reflux state at a heating rate of 5°C / min and mix for 15min,...

Embodiment 2

[0037](1) Preparation of co-fixative: add 0.5 g of microcrystalline cellulose and 0.05 g of ultrafine magnesium oxide to 2 g of poloxamer, and heat up to 115-120 ° C at a heating rate of 5 ° C / min and mix for 15 min. Then add 0.5g cationic polyacrylamide and 0.05g sodium alginate, continue to heat and mix at 115-120°C for 15min, transfer the resulting mixture to a sealed environment at 0-5°C for 1h, and heat up again at a rate of 5°C / min Heat up to 115-120°C and keep mixing for 15 minutes, then add 10g of 60-65°C water, mix well and keep it at 60-65°C for 30 minutes, filter, and send the obtained filter residue into a spray dryer, and dry the obtained particles through ultrafine Grinding machine into fine powder, that is, the co-fixative agent;

[0038] (2) Preparation of composite carrier: add 2.5g of ceramic micropowder, 0.3g of polyaspartic acid and 0.05g of sodium citrate to 10g of water, heat up to a reflux state at a heating rate of 5°C / min and mix for 15min, filter, a...

Embodiment 3

[0044] (1) Preparation of co-fixative: add 0.5 g of microcrystalline cellulose and 0.05 g of ultrafine magnesium oxide to 2 g of poloxamer, and heat up to 115-120 ° C at a heating rate of 5 ° C / min and mix for 15 min. Then add 0.5g cationic polyacrylamide and 0.05g sodium alginate, continue to heat and mix at 115-120°C for 15min, transfer the resulting mixture to a sealed environment at 0-5°C for 1h, and heat up again at a rate of 5°C / min Heat up to 115-120°C and keep mixing for 15 minutes, then add 10g of 60-65°C water, mix well and keep it at 60-65°C for 30 minutes, filter, and send the obtained filter residue into a spray dryer, and dry the obtained particles through ultrafine Grinding machine into fine powder, that is, the co-fixative agent;

[0045] (2) Preparation of composite carrier: add 2.5g of ceramic micropowder, 0.3g of polyaspartic acid and 0.05g of sodium citrate to 10g of water, heat up to a reflux state at a heating rate of 5°C / min and mix for 15min, filter, ...

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PUM

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Abstract

The invention discloses an amino-acid oxidase supported catalyst used for oxidizing amino acid to prepare ketonic acid and relates to the technical field of biological enzyme catalysts. A preparation method of the amino-acid oxidase supported catalyst includes the steps of firstly, preparing a co-immobilization agent; secondly, preparing a compound carrier; thirdly, preparing an activating carrier; fourthly, preparing the amino-acid oxidase supported catalyst. The prepared amino-acid oxidase supported catalyst can evidently increase the yield of the ketonic acid converted from the amino acid, the yield of an ketonic acid intermediate prepared from cephalosporin C is increased to above 95%, and the purity of the ketonic acid intermediate reaches above 99%.

Description

[0001] Technical field: [0002] The invention relates to the technical field of biological enzyme catalysts, in particular to an amino acid oxidase-supported catalyst for preparing ketoacids by oxidation of amino acids. [0003] Background technique: [0004] In the process of drug preparation, it is often necessary to convert the amino acid on the intermediate structure into a keto acid, which is an oxidation reaction. If chemical oxidants are used to participate in the reaction, there are problems that the degree of oxidation is not easy to control and the waste water generated by the reaction is difficult to handle, and chemical oxidants are more harmful to operators and have low safety in use. [0005] Amino acid oxidase is a special biological enzyme catalyst for the oxidation of amino acids to prepare keto acids. Under the action of amino acid oxidase, amino acids are converted into corresponding keto acids. At present, the method of using amino acid oxidase to catalyze...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/08B01J31/00
CPCB01J31/003C12N11/08C12N11/14
Inventor 孙凯王妍闫磊桂金秋蔡克瑞刘春玲齐悦卜晓波张书捷晁岳刚
Owner MUDANJIANG MEDICAL UNIV
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