Method for co-immobilization of nitrite bacteria-denitrifying bacteria

Abstract

The invention discloses a method for co-immobilization of nitrite bacteria-denitrifying bacteria, belonging to a method for co-immobilization of cells. The method comprises the following steps: (1) embedding the denitrifying bacteria by use of sodium alginate and calcium chloride first; (2) mixing immobilized globules prepared in step (1) with the nitrite bacteria and sodium alginate, and dripping into calcium chloride for second embedding; (3) inoculating the co-immobilized nitrite bacteria-denitrifying bacteria prepared in step (2) in a culture medium containing ammonia nitrogen for culturing, wherein the co-immobilized nitrite bacteria-denitrifying bacteria can be used for degradation of the ammonia nitrogen. The method has the advantages that 1, co-immobilization culture of the nitrite bacteria and the denitrifying bacteria in the same carrier is realized for the first time; 2, the method for preparing the co-immobilized globules is simple and convenient, the operation procedures are easy to control, and the prepared co-immobilized globules are easy to store and reuse; 3, due to the co-immobilization culture of the nitrite bacteria and the denitrifying bacteria, the co-immobilized nitrite bacteria-denitrifying bacteria have higher denitrification efficiency than the traditional strain which is not immobilized or immobilized simplex strain, and is easy to be separated from the water body and recyclable.

Description

technical field

[0001] The invention relates to a method for co-immobilizing cells, in particular to a method for co-immobilizing nitrosifying bacteria-denitrifying bacteria. Background technique

[0002] In recent years, many large enterprises and factories have discharged a large amount of untreated industrial sewage, such as coking wastewater, coal pressurized gasification wastewater, nitrogen fertilizer wastewater, etc., resulting in a sharp increase in nitrogen-containing compounds, resulting in serious nitrogen pollution. Causes a series of environmental problems such as eutrophication of water body and disorder of water body ecosystem, such as affecting the oxygen transfer of fish and some aquatic organisms in the water, causing their blood to bind oxygen, resulting in the death of fish and some aquatic organisms, serious Sometimes even destroy the ecological balance. Therefore, it is urgent to solve the problem of nitrogen pollution.

[0003] At present, the applic...