Method for co-immobilization of nitrite bacteria-denitrifying bacteria

A technology of denitrifying bacteria and nitrosifying bacteria, which is applied in the direction of fixing on/in the organic carrier, so as to achieve the effect of easy control of the operation procedure, simple method, and easy separation of water bodies

Inactive Publication Date: 2014-05-21
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] At present, the application of co-immobilized cell technology in nitrosifying bacteria and denitrifying bacteria needs further research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: With the strains of nitrosifying bacteria and denitrifying bacteria cultured in liquid as co-immobilization objects, immobilized cell technology is used to embed the denitrifying bacteria with sodium alginate and calcium chloride, and after embedding The immobilized pellets were mixed with nitrosating bacteria and sodium alginate, dropped into calcium chloride for the second embedding to form co-immobilized nitrosating-denitrifying bacteria, which can be used for the degradation of ammonia nitrogen.

[0025] Specific steps are as follows:

[0026] (1) Strain activation and liquid culture: The selected strains are Nitrosomonas, which are two types of nitrosifying bacteria and denitrifying bacteria Nitrosomonas sp. and aerobic pseudomonas Pseudomonas sp. After being activated by a conventional method, a liquid medium containing ammonia nitrogen is used to cultivate nitrosifying bacteria, and a liquid medium containing nitrate nitrogen is used to cultivate ...

Embodiment 2

[0032] Example 2: Co-immobilization of nitrosating-denitrifying bacteria

[0033] 1. Co-immobilization process

[0034] Select nitrosative bacteria that grow well in liquid medium, precipitate, discard the supernatant, take a certain amount of bacteria according to the ratio of nitrosative bacteria: sodium alginate = 1:1 (w / v), add sodium alginate Mix well, the final concentration of sodium alginate is 40-45g / L, use a No. 5 syringe to draw the mixture and drop it into 21g / L CaCl 2 In the solution, form small balls with a particle size of about 0.3 cm, and fix in a refrigerator at 4°C for 4 hours. The pellets were rinsed 2-3 times with deionized water. Select the denitrifying bacteria that grow well in the liquid medium, precipitate, discard the supernatant, insert 20 fixed nitrosifying bacteria pellets per gram of bacteria, mix well, and denitrifying bacteria: alginic acid Add sodium alginate at a ratio of 1:1 (w / v) and mix well. The final concentration of sodium alginate i...

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Abstract

The invention discloses a method for co-immobilization of nitrite bacteria-denitrifying bacteria, belonging to a method for co-immobilization of cells. The method comprises the following steps: (1) embedding the denitrifying bacteria by use of sodium alginate and calcium chloride first; (2) mixing immobilized globules prepared in step (1) with the nitrite bacteria and sodium alginate, and dripping into calcium chloride for second embedding; (3) inoculating the co-immobilized nitrite bacteria-denitrifying bacteria prepared in step (2) in a culture medium containing ammonia nitrogen for culturing, wherein the co-immobilized nitrite bacteria-denitrifying bacteria can be used for degradation of the ammonia nitrogen. The method has the advantages that 1, co-immobilization culture of the nitrite bacteria and the denitrifying bacteria in the same carrier is realized for the first time; 2, the method for preparing the co-immobilized globules is simple and convenient, the operation procedures are easy to control, and the prepared co-immobilized globules are easy to store and reuse; 3, due to the co-immobilization culture of the nitrite bacteria and the denitrifying bacteria, the co-immobilized nitrite bacteria-denitrifying bacteria have higher denitrification efficiency than the traditional strain which is not immobilized or immobilized simplex strain, and is easy to be separated from the water body and recyclable.

Description

technical field [0001] The invention relates to a method for co-immobilizing cells, in particular to a method for co-immobilizing nitrosifying bacteria-denitrifying bacteria. Background technique [0002] In recent years, many large enterprises and factories have discharged a large amount of untreated industrial sewage, such as coking wastewater, coal pressurized gasification wastewater, nitrogen fertilizer wastewater, etc., resulting in a sharp increase in nitrogen-containing compounds, resulting in serious nitrogen pollution. Causes a series of environmental problems such as eutrophication of water body and disorder of water body ecosystem, such as affecting the oxygen transfer of fish and some aquatic organisms in the water, causing their blood to bind oxygen, resulting in the death of fish and some aquatic organisms, serious Sometimes even destroy the ecological balance. Therefore, it is urgent to solve the problem of nitrogen pollution. [0003] At present, the applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04
Inventor 涂宝军董玉玮边阁苗敬芝徐笑笑肖杰朱雯雯
Owner XUZHOU UNIV OF TECH
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