Method for preparing ecological water purification particles mainly from denitrification biological microcapsules and sustained-release carbon sources

A technology of ecological water purification and slow-release carbon source, applied in chemical instruments and methods, biological water/sewage treatment, water pollutants, etc. Significant social benefits, improved biodegradability, and low prices

Active Publication Date: 2017-12-29
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a method for preparing ecological water purification particles based on denitrification biological microcapsules and slow-release carbon sources, which can effectively solve the problem of denitrification microorganisms in micro-polluted water bodies. Insufficient, low C / N, poor biodegradability, difficult to recycle and easy to cause secondary pollution

Method used

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  • Method for preparing ecological water purification particles mainly from denitrification biological microcapsules and sustained-release carbon sources
  • Method for preparing ecological water purification particles mainly from denitrification biological microcapsules and sustained-release carbon sources
  • Method for preparing ecological water purification particles mainly from denitrification biological microcapsules and sustained-release carbon sources

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Embodiment 1

[0031] In specific implementation, the present invention specifically comprises the following steps:

[0032] (1) Preparation of nitrogen-removing microorganisms

[0033] Inoculate Pseudomonas mendoza ( Pseudomonas mendocina ), Pseudomonas putida ( Pseudomonas putida ), the inoculation amount of Pseudomonas mendoza was 1% of the weight of the liquid medium, and the inoculation amount of Pseudomonas putida was 1% of the weight of the liquid medium. Under the conditions of 30±1°C and a rotational speed of 150r / min, Shake culture for 36 hours to form denitrification microorganisms;

[0034] (2), preparation of microcapsules

[0035] Prepare chitosan solution: dissolve chitosan in 1% acetic acid solution with a mass concentration of 1% at a constant temperature of 60°C to form a chitosan solution with a chitosan mass concentration of 0.5%;

[0036] Preparation of sodium alginate solution: Dissolve sodium alginate in water with constant temperature stirring at 90°C to prep...

Embodiment 2

[0045] In specific implementation, the present invention specifically comprises the following steps:

[0046] (1) Preparation of nitrogen-removing microorganisms

[0047] Introduce microbial strain heterotrophic nitrifying-aerobic denitrifying bacteria into the liquid medium ( Paracoccus pantotrophus ), the inoculum amount is 3% of the weight of the liquid culture medium, under the conditions of 30±1°C and a rotation speed of 150r / min, shake culture for 36h to form denitrification microorganisms;

[0048] (2), preparation of microcapsules

[0049] Prepare chitosan solution: dissolve chitosan in 1% acetic acid solution with a mass concentration of 1% at a constant temperature of 60°C to form a chitosan solution with a chitosan mass concentration of 1%;

[0050] Preparation of sodium alginate solution: Dissolve sodium alginate in water with constant temperature stirring at 90°C to prepare a sodium alginate solution with a mass concentration of 2.5%, and then add emulsif...

Embodiment 3

[0059] In specific implementation, the present invention specifically comprises the following steps:

[0060] (1) Preparation of nitrogen-removing microorganisms

[0061] The microbial strain Pseudomonas mendoza ( Pseudomonas mendocina ), heterotrophic nitrifying-aerobic denitrifying bacteria ( Paracoccus pantotrophus ), the inoculum amount is 2.5% of the weight of the liquid culture medium, under the conditions of 30±1°C and 150r / min, shake culture for 36h to form denitrification microorganisms;

[0062] (2), preparation of microcapsules

[0063] Prepare chitosan solution: dissolve chitosan in 1% acetic acid solution with a mass concentration of 1% at a constant temperature of 60°C to form a chitosan solution with a chitosan mass concentration of 1%;

[0064] Preparation of sodium alginate solution: Dissolve sodium alginate in water with constant temperature stirring at 90°C to prepare a sodium alginate solution with a mass concentration of 2-3%, then add emulsifie...

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Abstract

The invention relates to a method for preparing ecological water purification particles mainly from denitrification biological microcapsules and sustained-release carbon sources and effectively solves problems of denitrification microorganism insufficiency of micropollution water, low C/N ratio, poor biodegradability, difficulty in recovery and secondary pollution. The method includes: culturing a microbial strain to obtain denitrification microorganisms; dissolving chitosan into acetic acid solution to obtain chitosan solution; dissolving sodium alginate into water, and adding span 80 to obtain sodium alginate solution; well mixing the sodium alginate solution with the denitrification microorganisms, dropwise adding CaCl2 solution to obtain calcium alginate capsules, and putting the calcium alginate capsules into the chitosan solution to obtain microcapsules through secondary solidifying; putting the microcapsules into sodium citrate solution, and performing interlayer calcium alginate liquefying to obtain biological microcapsules; well mixing hydrophilic materials, pore-forming materials, adhesives, the sustained-release carbon sources and the biological microcapsules to obtain spherical ecological water purification particles, and curing. The method has advantages of rich materials, low cost, freeness of toxicity and harms to microorganisms, recyclability and freeness of secondary pollution.

Description

technical field [0001] The invention relates to chemical industry, in particular to a method for preparing ecological water purification granules mainly based on denitrification biological microcapsules and slow-release carbon sources. Background technique [0002] Water nitrogen pollution is one of the important environmental problems. Rivers, lakes and groundwater in various parts of my country are polluted by nitrogen to varying degrees. The degradation and removal of nitrogen is an important goal of ecological restoration of polluted water bodies. The removal of nitrogen in water is mainly achieved by denitrification microorganisms, while the common sewage in my country is low C / N sewage, which has poor biodegradability and limits the conversion and removal of nitrogen. Therefore, providing a certain number of denitrification microorganisms and supplementing carbon sources has become a solution One of the effective methods of water nitrogen pollution. [0003] At present...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F1/28C02F101/38C02F101/34
CPCC02F1/281C02F3/34C02F2101/34C02F2101/38
Inventor 于鲁冀程璐璐刘露李廷梅范鹏宇刘攀龙
Owner ZHENGZHOU UNIV
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