Method for preparing sodium gluconate through co-immobilization of glucose oxidase and catalase

A technology of glucose oxidase and catalase, which is applied in biochemical equipment and methods, oxidoreductase, immobilized on/in organic carriers, etc., can solve the problem of high sterilization degree requirements and is not suitable for food applications and medicine, limiting yield, output, quality and other issues, to achieve the effect of not producing three wastes pollution, reducing production costs, and high purity

Inactive Publication Date: 2017-05-31
温县兴发生物科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological fermentation method has the advantages of easy process control, easy extraction of products, and high product purity. However, the degree of sterility is high and it is easily affected by external factors (bacteria quality, process conditions, climate change), which limits the yield and output. , qu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0018] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction. During the reaction, adjust the pH to 6.5 with sodium hydroxide, and obtain sodium gluconate after neutralization;

[0019] (3) After the pH was stabilized at 6.5, sodium gluconate was obtained by ion exchange and drying.

[0020] In the step (1), the conditions for the cross-linking and esterification reaction are as follows: the reaction time is 8 hours in total, of which 0-4 hours are at a temperature of 35°C; 4-8 hours are at a temperature of 4°C; gluconolactone, calcium...

Embodiment 2

[0023] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0024] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction. During the reaction, adjust the pH to 6.5 with sodium hydroxide, and obtain sodium gluconate after neutralization;

[0025] (3) After the pH was stabilized at 6.5, sodium gluconate was obtained by ion exchange and drying.

[0026] In the step (1), the conditions for the cross-linking and esterification reaction are as follows: the reaction time is 8 hours in total, of which 0-4 hours, the temperature is 38 ° C, and 4-8 hours, the temperature is 6 ° C; gluconolactone, calcium...

Embodiment 3

[0029] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0030] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction. During the reaction, adjust the pH to 6.5 with sodium hydroxide, and obtain sodium gluconate after neutralization;

[0031] (3) After the pH was stabilized at 6.5, sodium gluconate was obtained by ion exchange and drying.

[0032] In the step (1), the conditions for the cross-linking and esterification reactions are: the reaction time is 8 hours in total, of which 0-4 hours are at a temperature of 40°C, and 4-8 hours are at a temperature of 8°C; gluconolactone, calcium algin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for preparing sodium gluconate through co-immobilization of glucose oxidase and catalase. Glucose is used as a substrate, and glucolactone, calcium alginate and chitosan are adopted for co-immobilization of glucose oxidase and catalase so as to achieve catalytic oxidation of glucose, so that sodium gluconate is prepared. By the adoption of the technique, enzyme activity is improved remarkably, the number of transformation steps is reduced, the glucose conversion rate is increased greatly, energy consumption is reduced, and production cost is reduced.

Description

technical field [0001] The invention relates to a preparation method of sodium gluconate, in particular to a method for co-immobilizing glucose oxidase and catalase to prepare sodium gluconate. Background technique [0002] Gluconic acid is the oxidation product of glucose. After derivatization, a series of gluconic acid products can be obtained, including gluconic acid (sodium, calcium, magnesium, copper, manganese, ferrous), gluconolactone and other products, gluconic acid (salt) series Products are widely used in the fields of food, medicine, chemical industry, etc. Sodium gluconate has good thermal stability, no deliquescence, excellent chelation, and is also an intermediate in the synthesis of gluconic acid (salt), so its market potential is huge and its application prospects are very broad. At present, it is mainly used in medicine and food. , construction, industry, etc. The two methods of biological fermentation and heterogeneous catalytic oxidation are currently u...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N11/10C12N11/04C12N11/02C12N9/04C12P7/58
CPCC12N11/10C12N9/0006C12N11/02C12N11/04C12P7/58C12Y101/03004C12Y111/01006
Inventor 吴丽安民安庆堂冯斌斌
Owner 温县兴发生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap