Method for preparing calcium gluconate through co-immobilization of glucose oxidase and catalase

A technology of glucose oxidase and catalase, applied to biochemical equipment and methods, oxidoreductase, immobilized on/in organic carriers, etc., can solve the problems of large production fluctuations, many steps, limited output, Yield, quality improvement and other issues, to achieve the effect of no three waste pollution, high purity, and reduce production cost

Inactive Publication Date: 2017-04-26
温县兴发生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The fermentation method is affected by various factors such as the quality of the strain, expansion, contamination, process changes, and...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0018] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction. During the reaction, calcium carbonate is used to adjust the pH=6.5, and calcium gluconate is obtained after neutralization;

[0019] (3) After the pH is stabilized at 6.5, calcium gluconate is obtained by ion exchange and drying.

[0020] In the step (1), the conditions for the cross-linking and esterification reaction are as follows: the reaction time is 8 hours in total, of which 0-4 hours are at a temperature of 35°C; 4-8 hours are at a temperature of 4°C; gluconolactone...

Embodiment 2

[0023] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0024] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction. During the reaction, calcium carbonate is used to adjust the pH=6.5, and calcium gluconate is obtained after neutralization;

[0025] (3) After the pH is stabilized at 6.5, calcium gluconate is obtained by ion exchange and drying.

[0026] In the step (1), the conditions for the cross-linking and esterification reaction are as follows: the reaction time is 8 hours in total, of which 0-4 hours, the temperature is 38 ° C, and 4-8 hours, the temperature is 6 ° C; gluconolactone...

Embodiment 3

[0029] (1) Co-immobilization of glucose oxidase and catalase: take glucose oxidase / catalase (U / U) = 1:1, add gluconolactone and seaweed under the condition of pH 7.0 Calcium acid and chitosan carry out cross-linking and esterification reactions, stirring during the reaction, and preparing microspheres with a diameter of 0.1-0.12 cm after the reaction is completed;

[0030] (2) Fix the microspheres in the enzymatic hydrolysis tank, use glucose as the substrate, and carry out the immobilized enzyme reaction. During the reaction, calcium carbonate is used to adjust the pH=6.5, and calcium gluconate is obtained after neutralization;

[0031] (3) After the pH is stabilized at 6.5, calcium gluconate is obtained by ion exchange and drying.

[0032] In the step (1), the conditions for the cross-linking and esterification reactions are: the reaction time is 8 hours in total, of which 0-4 hours are at a temperature of 40°C, and 4-8 hours are at a temperature of 8°C; gluconolactone, calc...

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Abstract

The invention discloses a method for preparing calcium gluconate through co-immobilization of glucose oxidase and catalase. With glucose as a substrate, a technology adopting glucolactone, chitosan and calcium alginate for co-immobilization of glucose oxidase and catalase for catalytic oxidation of glucose is used for preparing calcium gluconate; the technology can significantly improve the enzyme activity and reduce conversion steps, the sugar conversion rate is greatly increased, energy consumption is saved, and the production cost is reduced.

Description

technical field [0001] The invention relates to a preparation method of calcium gluconate, in particular to a method for co-immobilizing glucose oxidase and catalase to prepare calcium gluconate. Background technique [0002] Calcium gluconate, the molecular formula is Ca(C 6 h 11 o 7 ) 2 , the product is white crystalline or granular powder, melting point 201°C (decomposition), odorless, tasteless, easily soluble in boiling water (20g / 100ml), slightly soluble in cold water (3g / 100ml, 20°C), insoluble in ethanol Or organic solvents such as ether. The aqueous solution is neutral (pH about 6-7). Calcium gluconate is mainly used as calcium fortifier and nutrient, buffer, curing agent and chelating agent in food. As a drug, calcium gluconate can reduce capillary permeability, increase density, maintain normal excitability of nerves and muscles, strengthen myocardial contractility, and help bone formation. It is suitable for allergic diseases, such as urticaria; eczema; sk...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C12N11/02C12N9/04C12P7/58
CPCC12N9/0006C12N11/02C12N11/04C12N11/10C12P7/58C12Y101/03004C12Y111/01006Y02P20/50
Inventor 吴丽安民安庆堂冯斌斌
Owner 温县兴发生物科技有限公司
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