Immobilized enzyme and preparation method thereof

A technology for immobilizing enzymes and lipases, applied in the fields of immobilized enzymes, immobilized enzymes and their preparation, and preparation of immobilized enzymes, which can solve the problems of lipase immobilized enzymes that need to be improved and few studies on co-immobilized lipases.
CN110004138AInactive Publication Date: 2019-07-12CHINESE ACAD OF INSPECTION & QUARANTINE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINESE ACAD OF INSPECTION & QUARANTINE
Publication Date
2019-07-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an immobilized enzyme and a preparation method thereof. The preparation method of the immobilized enzyme comprises the following steps: dissolving ferroferric oxide in a firstbuffer solution to obtain a base material solution, and adding a dopamine salt for coating treatment so as to obtain a polydopamine coated ferroferric oxide nanocomposite; dissolving nonspecific lipase and sn-1,3 specific lipase in a second buffer solution so as to obtain an enzyme buffer solution; enabling the polydopamine coated ferroferric oxide nanocomplex to be in contact with the enzyme buffer solution, and obtaining the immobilized double enzyme through covalent fixation. According to the method, the ferroferric oxide nanocomposite is used as a fixed carrier, and the sn-1,3 specific lipase and the nonspecific lipase are subjected to co-immobilization treatment to obtain the immobilized double enzyme. The preparation process is simple, the conditions are mild, meanwhile the stabilityof the prepared immobilized double enzyme is good, the catalytic effect is high, the separation and recovery are easy, the repeated use effect is good, and the enzyme is convenient for industrial production.
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Description

technical field

[0001] The present invention relates to the field of biology, specifically, optionally, an immobilized enzyme and a preparation method thereof, more specifically, a method for preparing an immobilized enzyme and an immobilized enzyme. Background technique

[0002] As a special biocatalyst, lipase can not only promote the hydrolysis of oil, but also accelerate the synthesis of oil under certain conditions and undergo transesterification. It is widely used in the fields of food, medicine and biodiesel. However, in the actual use process, the use of free enzymes has great limitations, such as enzymes are not stable enough under conditions such as heat, strong acid, strong alkali, organic solvents, etc., easily lose their catalytic activity, and there are difficulties in separation and purification, and difficult to recycle Repeated use will cause product pollution and increase production costs. Immobilization technology can solve the above problems. The immobi...

Claims

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