Preparation method for Trichoderma harzianum degrading p-toluenesulfonic acid and industrial waste water treatment method

A technology of Trichoderma harzianum and p-toluenesulfonic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of secondary pollution, high toxicity of intermediate products, etc., and achieve the goal of reducing pollution Effect

Inactive Publication Date: 2015-12-23
JIAXING UNIV
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Problems solved by technology

Use other chemical, photochemical and physical chemical means to solve the pollution of p-toluenesulfonic acid, but this will cause secondary pollution or only transfer pollutants from one environmental system to another, such as photolysis and chemical oxidation, due to The excitation effect of light energy and chemical energy on the intermediate product makes the intermediate product sometimes more toxic than before treatment

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  • Preparation method for Trichoderma harzianum degrading p-toluenesulfonic acid and industrial waste water treatment method
  • Preparation method for Trichoderma harzianum degrading p-toluenesulfonic acid and industrial waste water treatment method
  • Preparation method for Trichoderma harzianum degrading p-toluenesulfonic acid and industrial waste water treatment method

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Embodiment Construction

[0021] The present invention will be further illustrated by specific embodiments below.

[0022] The Trichoderma harzianum that degrades p-toluenesulfonic acid according to the present invention is a filamentous fungal strain named Trichodermaharzianum 1228, which is preserved in the Chinese Type Culture Collection at Wuhan University, Luojiashan, Wuhan ( CCTCC), the preservation date is June 27, 2015, and the preservation number is CCTCCM2015412. The strain was isolated and screened from straw, and was identified as Trichoderma harzianum.

[0023] The physiological and biochemical properties of Trichodermaharzianum 1228 are:

[0024] The strain can grow at 15-40℃, and its optimum temperature is 25-30℃; at pH 4-8, it can grow with optimum pH 5-6; sucrose, mannose, glucose, Xylose and starch are conducive to the growth of mycelia, maltose and D-galactose are poor; ammonium nitrate, urea, amino acids, soybean meal and other inorganic or organic nitrogen are conducive to the growth of...

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Abstract

The invention discloses a preparation method for Trichoderma harzianum degrading p-toluenesulfonic acid. A solid fermentation method is used for preparing the Trichoderma harzianum, and the preparation method includes the steps that straw powder is added into a fermentation vat, water and a nitrogen source are added and stirred sufficiently and evenly, and the mixture is inoculated with Trichoderma harzianum strains and make full contact with the straw powder; fermentation is carried out under the natural condition, and the temperature of a fermentation substrate is controlled not to exceed 50 DEG C. When laccase activity in the substrate is not increased, fermentation is stopped, and the obtained fermentation substrate is the Trichoderma harzianum used for treating industrial waste water. An industrial waste water treatment method with the Trichoderma harzianum includes the step that the additive amount of the Trichoderma harzianum in industrial waste water accounts for 0.1-5% of the waste water amount; the pH value of the industrial waste water is 3.0-6.0, the treatment temperature is 20 DEG C-50 DEG C, and the treatment time is 2-10 h.

Description

Technical field [0001] The invention relates to the fields of environmental protection and biochemical industry, in particular to a method for preparing and treating industrial wastewater by degrading Trichoderma harzianum p-toluenesulfonate. Background technique [0002] P-toluenesulfonic acid is widely used as a stabilizer for the synthesis of medicines, pesticides, polymerization reactions and a catalyst for organic synthesis (esters, etc.); as an intermediate for medicines and coatings and as a resin curing agent, as well as an intermediate for electroplating. P-toluenesulfonic acid has a strong irritating effect on the eyes, skin, mucous membranes and upper respiratory tract; after inhalation, it can cause spasm, edema of the larynx and bronchi, chemical pneumonia or pulmonary edema. Poisoning manifestations include burning sensation, cough, wheezing, and laryngitis , Shortness of breath, headache, nausea and vomiting. The acute toxicity test shows that the LD50 of p-toluen...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/885C02F101/34
Inventor 李加友沈洁屠立冬王宏亮吴建一
Owner JIAXING UNIV
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