Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof

An establishment method and a technology for the variegated jade fungus are applied in the field of establishment of the laccase transformation system in the variegated jade fungus and its breeding, which can solve the problems of slow growth rate, fast growth speed, long production cycle and the like, and achieve fast growth speed and production The effect of short cycle and heavy workload

Active Publication Date: 2010-02-10
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for establishing a laccase transformation system in the jaundice mushroom and its breeding, to solve the problem that the jaundice mushroom in the prior art does not have laccase activity, the growth rate is slow, the production cycle is long, and the antibacterial Weak problem, the new bacterial strain of Japonica japonica cultivated by the method of the present invention has excellent properties such as high laccase activity, fast growth rate, and short production cycle

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  • Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof

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Embodiment

[0015] The specific steps include:

[0016] a: Separately cultivate the Pleurotus ostreatus strain with laccase activity and the general Pleurotus ostreatus strain:

[0017] (1) Observation and comparison of mycelium morphology of Pleurotus ostreatus and Pleurotus ostreatus:

[0018] The two kinds of bacteria were respectively inoculated on a PDA plate and cultured at a constant temperature of 25°C for 4-6 days. On the second day of inoculation, the new hyphae began to germinate, and the hyphae grew thick and dense in a radial shape. A little hyphae were produced only after 4 days. The hyphae are thinner and not as dense as the hyphae of Pleurotus ostreatus. Use an inoculation needle to pick out the young hyphae of Pleurotus ostreatus and Pleurotus edulis, dye them with cotton and make a glass slide. Under an optical microscope, observe the growth of the top of the hyphae, the nuclear phase and the presence or absence of lock-like union. Observation found that the top of the myceli...

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Abstract

The invention provides hypsizigus marmoreus and a method for establishing laccase transfer system in breeding thereof, belongs to the technical field of the hypsizigus marmoreus, and solves the problems that the hypsizigus marmoreus does not have laccase activity, has slow growing speed, longer production period and weak bacterial resistance in the prior art. The Hypsizigus marmoreus new strain Jinshan No.1 has the laccase activity, and is reserved by China General Microbiological Culture Collection Center on 12 June, 2009 with the accession number of CGMCC No. 3120. Pleurotus pulmonarius withthe laccase activity and the hypsizigus marmoreus are subjected to protoplast fusion to establish the laccase transfer system in the hypsizigus marmoreus. The hypsizigus marmoreus new strain culturedby the method has excellent properties of high laccase activity, high growing speed, short production period and the like.

Description

Technical field [0001] The present invention belongs to the technical field of Candidae, and more specifically relates to a method for establishing a laccase conversion system in Candidae and its breeding. Background technique [0002] Hypsizigus marmoreus (Hypsizigus marmoreus) belongs to the Basidiomycotina subdivision, Layer Fungi, Agaricales, Tricholomaceae, and Yumushroom. It is a rare and valuable edible mushroom with high economic value; but it has a long period of cultivation and production. , The growth rate of the bacteria is slow and the ability to inhibit the miscellaneous bacteria is poor, and it is very easy to contaminate the bacteria in the production process, which reduces the utilization rate of raw materials and increases the production cost, which limits the promotion and production of Panyu mushroom to a certain extent. Laccase is a copper-containing polyphenol oxidase, belonging to the blue polycopper oxidase family. It can oxidize phenols and aromatic compo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H15/00C12N15/04C12Q1/26C12Q1/68
Inventor 胡开辉孙淑静刘建忠饶榆平陈明祥熊芳
Owner FUJIAN AGRI & FORESTRY UNIV
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