Laccase biosensor based on magnetic carbon nano tube and chitosan/silicon dioxide gel and preparation method and application thereof

Abstract

The invention discloses a laccase biosensor based on magnetic carbon nano tube and chitosan / silicon dioxide gel, comprising a magnetic carbon paste electrode, the electrode surface of the magnetic carbon past electrode is modified with a magnetic carbon nano tube layer, the surface of the magnetic carbon nano tube layer is absorbed with laccase, the laccase is wrapped by a chitosan-carbon dioxide composite film; the preparation method of the laccase biosensor includes the steps of material preparation, magnetic carbon nano tube modification, laccase absorption and laccase wrapping. The method for detecting pyrocatechol by applying the laccase biosensor includes the following steps: the laccase biosensor is taken as a working electrode, a three electrode system is established, the three electrode system is connected with an electrochemical work station, and reduction current amount is detected to quantitatively indicate the concentration of pyrocatechol in solution to be detected. The sensor of the invention has the advantages of low cost, simple manufacturing, long service life, high laccase activity, high detection accuracy and strong anti-interference.

Description

technical field [0001] The invention relates to the field of biosensors, in particular to a laccase biosensor and its preparation method and application. Background technique [0002] Biosensors are based on biological organic components (such as enzymes, antibodies, nucleic acids, cells, microorganisms, etc.) to specifically identify the substance to be tested, and the generated signal is converted into an electrical signal and an optical signal through a signal transducer to detect the substance to be tested A new technology in the amount of The use of biosensors to detect heavy metals, pathogenic microorganisms, and harmful organic substances in the environment has the characteristics of strong specificity, high detection sensitivity and efficiency, and low cost, so it has become a research hotspot in environmental protection. At present, researchers are working hard to improve the stability, repeatability and structural reliability of biosensors. Among them, the use of ...

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Problems solved by technology

Commonly used enzyme immobilization methods include: adsorption immobilization method, embedding immobilization method, covalent immobilization method and cross-linking immobilization method. These methods have the disadvantages that the immobilization is not firm, the cross-linking agent used in the immobilization affects the activity of the enzyme, and it is easy to use. Defects such as environmentally harmful substances

[0004] The existing method for detecting catechol concentration mainly adopts high performance liquid chromatography (HPLC), and its basic principle is to calculate its content according to the peak area produced after catechol is adsorbed in the mobile phase, but this The instrument required by the method is expensive, the operation is complicated, the sample has to undergo tedious pretreatment, and is easily affected by turbidity and light interfering substances

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