Thermus thermophilus and saccharomycetes combined fermentation method

A thermophilic thermophilic bacteria and combined fermentation technology, applied in the field of cosmetic fermentation raw materials, can solve the problems of unsatisfactory actual effect, loss of bacteria and enzymes, uncertain enzyme activity, etc.

Active Publication Date: 2018-09-11
HANGZHOU UMOTOR BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thermophilic bacteria are generally produced by fermentation, and the activity or yield of the enzyme is not disclosed, and the stability of this enzyme is also an important factor when it is combined with other ingredients. After all, in sunscreen products Contains enzymes, whether the enzymes are inactivated by ultraviolet light, whether they have enzymatic activity is uncertain
[0005] In the existing traditional technology centers, after the fermentation of a single strain is completed separately, and then the two fermentation broths are blended, different flora and enzymes dissolve together to produce an offset reaction, which will lose a large amount of flora and Enzymes, often the actual effect is not ideal

Method used

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  • Thermus thermophilus and saccharomycetes combined fermentation method
  • Thermus thermophilus and saccharomycetes combined fermentation method
  • Thermus thermophilus and saccharomycetes combined fermentation method

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Embodiment Construction

[0051] The present invention illustrates how to realize the present invention through specific implementation modes, and these descriptions are only descriptions of the essence of the present invention, and cannot limit the present invention in any way. The specific scope of protection is subject to the claims.

[0052] Implementation example 1: combined fermentation method of thermophilic Thermus and saccharomyces (1)

[0053] Material:

[0054] Preparation and composition of culture medium: The ratio of 20L culture medium is 60g peptone, 4g wort juice, 7g anhydrous magnesium sulfate, 2.4g potassium dihydrogen phosphate, 40g ammonium sulfate, 1g anhydrous ferric chloride, 5g sodium chloride, pH The value is adjusted and controlled with acid or alkali (hydrochloric acid or sodium hydroxide solution), water is added to 20L, and different amounts of culture solution are reduced or enlarged according to the proportion. The present invention does not specifically specify that a...

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Abstract

The invention discloses a thermus thermophilus and saccharomycetes combined fermentation method including the steps of: (1) performing one-time or multi-time fermentation to saccharomyces cerevisiae at medium temperature under acidic condition, thus reproducing the saccharomyces cerevisiae; (2) when the saccharomyces cerevisiae is in later period of logarithmic phase, increasing the temperature, inoculating the thermus thermophilus, and performing secondary fermentation under alkaline condition, thereby producing a fermentation product. By means of the method, active components in the thermusthermophilus can be significantly increased; especially, active components, which are applied to cosmetics, are increased in content or improved in activity.

Description

technical field [0001] The invention belongs to the field of cosmetic fermentation raw materials, and in particular relates to a method and application of combined fermentation of thermophilic bacteria and saccharomyces. Background technique [0002] There are more and more applications of bio-fermented raw materials in the field of cosmetics. In the past two years, enzyme cosmetics have been sought after by consumers. Fermentation is a purely biological process without any chemical ingredients added in the middle. Fermentation makes the active substances dissolve more easily. Extracting all the active ingredients through fermentation will produce new nutrients, make the nutrients more balanced, make the nutrients small molecules, more active, and can be quickly absorbed by the skin. Fermentation microorganisms themselves contain many enzymes and trace elements, which can supplement and synergize. Meet consumers' expectations for "natural, healthy and efficient" skin care ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12N1/20C12N1/18A61K8/99A61K8/9728A61Q19/00C12R1/865C12R1/01
CPCA61K8/99A61K2800/85A61Q19/00A61K8/9728C12N1/18C12N1/20C12P39/00
Inventor 郑婷高颖姚雨辰章坤鹏
Owner HANGZHOU UMOTOR BIOTECH CO LTD
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