Fermentation conversion method and application of madecassoside

Abstract

The invention relates to a centella asiatica fermentation liquor, a preparation method and application of the centella asiatica fermentation liquor, wherein the centella asiatica fermentation liquor can be used for converting high-content madecassoside and producing high-activity asiatic acid and derivatives of the high-activity asiatic acid by utilizing a microbial conversion reaction. After centella asiatica is fermented by thermus thermophilus, the centella asiatica is converted to generate the madecassoside and asiatic acid, the content of madecassoside is increased by 200-8000 times, and the effects of the fermented centella asiatica, such as inflammation resistance, wound repair, oxidation resistance, whitening and skin cell repair capability (light damage repair and scratch repair) are also remarkably improved.

Description

technical field [0001] The invention belongs to the field of fermentation, and relates to the fermentation of centella asiatica, in particular to a preparation method for transforming rich madecassoside by using microbial transformation reaction, and the application of madecassoside fermented liquid. Background technique [0002] Centella asiatica is Centella asiatica (Latin name: Centellaasiatica (L.) Urban), also known as gotu kola, copper money grass, and horseshoe grass, which is the whole herb of the Umbelliferae plant Centella asiatica. Centella Asiatica is native to India and is now widely distributed in tropical and subtropical regions of the world. In my country, it is mainly distributed in the provinces south of the Yangtze River. The main active components of Centella asiatica whole grass are asiaticoside, madecassoside, asiatic acid and madecassic acid. Centella asiatica has long been used as a brain tonic, diuretic, leprosy, adjuvant for ulcers, and skin diseas...

AI Technical Summary

Problems solved by technology

However, this method starts from the raw material containing asiaticoside and adopts a simulated moving bed chromatographic system. The operation is complicated, the equipment requirements are high, and methyl tert-butyl ether is used, which has certain toxicity and danger. and in the process of using it, we need to pay full attention to

[0006] CN102477063 discloses a method for separating asiaticoside B and hydroxyasiaticoside in centella asiatica extract by a preparative high-performance liquid chromatograph, adding cyclodextrin in the mobile phase, and then extracting with an organic solvent to remove the asiaticoside B and hydroxyasiaticoside in the solvent Cyclodextrin to obtain pure products, the method is complicated to operate, adding β-cyclodextrin solvent will easily cause clogging of the chromatographic column, reduce the service life of the chromatographic column, the cost is high, and it is not easy for industrial production

CN102532244A discloses a method for preparing high-purity asiaticoside. Pure asiaticoside is obtained by extraction, decolorization and recrystallization. However, various organic solvents are used in the process to extract the extract of centella asiaticoside. The amount of solvent used is large, which is likely to cause secondary pollution

CN101948501A discloses a method for preparing madecassoside. The extract is subjected to macroporous resin column layer, activated carbon decolorization, crystallization, and macroporous resin re-chromatography to finally obtain madecassoside with a purity of more than 98%. Long cycle, complicated process and high cost

However, the use of microorganisms to ferment Centella asiatica to obtain madecassoside through its biotransformation has not been reported at present

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