Fermentation conversion method and application of madecassoside

A technology of madecassoside and asiaticoside, which is applied to the application of madecassoside fermentation liquid and the field of centella asiaticoside fermentation, and can solve the problems of high cost, complicated operation, and large amount of organic solvents

Active Publication Date: 2021-06-25
HANGZHOU UMOTOR BIOTECH CO LTD +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, this method starts from the raw material containing asiaticoside and adopts a simulated moving bed chromatographic system. The operation is complicated, the equipment requirements are high, and methyl tert-butyl ether is used, which has certain toxicity and danger. and in the process of using it, we need to pay full attention to
[0006] CN102477063 discloses a method for separating asiaticoside B and hydroxyasiaticoside in centella asiatica extract by a preparative high-performance liquid chromatograph, adding cyclodextrin in the mobile phase, and then extracting with an organic solvent to remove the asiaticoside B and hydroxyasiaticoside in the solvent Cyclodextrin to obtain pure products, the method is complicated to operate, adding β-cyclodextrin solvent will easily cause clogging of the chromatographic column, reduce the service life of the chromatographic c

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  • Fermentation conversion method and application of madecassoside
  • Fermentation conversion method and application of madecassoside
  • Fermentation conversion method and application of madecassoside

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[0044] The invention provides a fermentative transformation method of madecassoside. The preparation method comprises: using Centella asiatica or the crude extract of Centella asiatica or asiaticoside or a combination thereof as a raw material, by Microbial fermentation to prepare Centella asiatica fermented liquid rich in madecassoside;

[0045] Alternatively, the Centella asiaticoside fermented liquid rich in madecassoside is prepared by using the centella asiatica fermented liquid or the crude extract of centella asiatica or asiaticoside as a raw material through secondary fermentation of microorganisms;

[0046] post-processing the fermentation broth rich in madecassoside to obtain pure madecassoside;

[0047] Wherein, the microorganisms include thermophilic bacteria or a multi-bacteria composition composed of thermophilic bacteria and non-thermophilic bacteria.

[0048] Preferably, the thermophilic bacteria include one of Thermus thermophilus, Streptococcus thermophilus,...

Embodiment 1

[0057] Centella asiatica was added to the medium of Thermus thermophiles, and cultured and fermented according to the fermentation process of Thermus thermophiles (60°C, 48h). Two concentrations of Centella asiatica were selected: 5g / L, 50g / L And with the same concentration of Centella asiatica water extract (60°C, 48h), and the concentrated solution of the same concentration of Centella asiatica water extract (concentrated into a medicinal material: concentrated solution ratio of 2:1, that is, 2g of medicinal materials to obtain a concentrated solution 1g) and the pure Thermus thermophilus fermented liquid without adding Centella asiatica were analyzed and compared, the results are shown in Table 1 and figure 1 , figure 2 : (1) Comparative data of various ingredients

[0058] Table 1 Composition comparison of Centella asiatica fermented liquid and pure thermophilic Thermus fermented liquid, Centella asiatica water extract, same concentration of Centella asiatica water extra...

Embodiment 2

[0076] Add asiaticoside (addition amount 50g / L) to the culture medium of the dual-bacteria composition of thermophilic bacteria and lactic acid bacteria, first ferment the lactic acid bacteria at (28°C, 18h) according to the fermentation process, and then transfer to Fermentation of Thermus thermica (78°C, 8h) for cultivation and fermentation, adding the fermented liquid to D330 resin to decolorize at a flow rate of 2BV / H, after centrifugal filtration, to obtain Centella asiatica fermented liquid rich in madecassoside, and then The pure product of madecassoside is obtained through post-treatment processes such as conventional purification and crystallization.

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Abstract

The invention relates to a centella asiatica fermentation liquor, a preparation method and application of the centella asiatica fermentation liquor, wherein the centella asiatica fermentation liquor can be used for converting high-content madecassoside and producing high-activity asiatic acid and derivatives of the high-activity asiatic acid by utilizing a microbial conversion reaction. After centella asiatica is fermented by thermus thermophilus, the centella asiatica is converted to generate the madecassoside and asiatic acid, the content of madecassoside is increased by 200-8000 times, and the effects of the fermented centella asiatica, such as inflammation resistance, wound repair, oxidation resistance, whitening and skin cell repair capability (light damage repair and scratch repair) are also remarkably improved.

Description

technical field [0001] The invention belongs to the field of fermentation, and relates to the fermentation of centella asiatica, in particular to a preparation method for transforming rich madecassoside by using microbial transformation reaction, and the application of madecassoside fermented liquid. Background technique [0002] Centella asiatica is Centella asiatica (Latin name: Centellaasiatica (L.) Urban), also known as gotu kola, copper money grass, and horseshoe grass, which is the whole herb of the Umbelliferae plant Centella asiatica. Centella Asiatica is native to India and is now widely distributed in tropical and subtropical regions of the world. In my country, it is mainly distributed in the provinces south of the Yangtze River. The main active components of Centella asiatica whole grass are asiaticoside, madecassoside, asiatic acid and madecassic acid. Centella asiatica has long been used as a brain tonic, diuretic, leprosy, adjuvant for ulcers, and skin diseas...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P33/20A61K8/60A61K8/9789A61Q19/00A61Q19/02A61Q19/08C12R1/46C12R1/225C12R1/07C12R1/01C12R1/69
CPCC12P39/00C12P33/20A61K8/602A61K8/9789A61Q19/00A61Q19/02A61Q19/08A61Q19/004A61K2800/85
Inventor 魏建良颜贵卉姚雨辰章鹏坤全莉红王旻子张明洲
Owner HANGZHOU UMOTOR BIOTECH CO LTD
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