A composition against ultraviolet damage and its use in sunscreen products

Abstract

The present application relates to a kind of anti-ultraviolet damage composition and its application in sunscreen product, the anti-ultraviolet damage composition includes the following raw materials by weight parts: 5-8 parts of daphne giraldii leaf extract, 3-7 parts of green algae extract, 1-4 parts of dogwood extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of avocado butter and 5-8 parts of tea seed oil, each raw material has synergistic effect, by neutralizing the free radical induced by ultraviolet, reduce oxidative stress, protect skin cells from damage;Absorb ultraviolet, reduce the penetration of ultraviolet through skin, thereby reducing the direct damage of ultraviolet to skin;Regulate tyrosinase activity or block the melanin synthesis pathway, reduce the accumulation of melanin, help to prevent and improve the efficacy of anti-ultraviolet damage such as pigmentation, dull and other ways.

Description

Technical Field

[0001] This invention relates to the field of daily chemical technology, specifically to a composition for resisting ultraviolet damage and its application in sunscreen products. Background Technology

[0002] Ultraviolet (UV) radiation consists of short-wave UVC, medium-wave UVB, and long-wave UVA. UVA accounts for approximately 4.9% of the sun's energy and can penetrate to the dermis, causing skin aging, pigmentation, darkening, and potentially even skin cancer. UVB can penetrate the skin's surface, causing blood vessels to dilate, resulting in localized redness and heat—the most common symptoms of sunburn. To mitigate the damage caused by UV radiation, skincare products incorporate compositions that combat UV damage. These compositions form a protective layer on the skin's surface, blocking harmful rays such as UV rays and thus providing protection. Summary of the Invention

[0003] In view of this, a composition for resisting ultraviolet damage is provided to solve the above-mentioned problems.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0005] An anti-ultraviolet damage composition, by weight, comprises the following ingredients: 5-8 parts of cowhide tea extract, 3-7 parts of green algae extract, 1-4 parts of cornus extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of shea butter, and 5-8 parts of camellia seed oil.

[0006] Rhododendron chrysanthum Pall. extract: Rhododendron chrysanthum, also known as cowhide tea, contains active substances such as chlorogenic acid, quercetin, and hyperoside. Chlorogenic acid can scavenge free radicals in the body and reduce oxidative stress damage; quercetin has antioxidant effects, protecting lipids in the skin epidermis from UV degradation, thereby strengthening the skin barrier and reducing moisture loss.

[0007] Green algae extract is rich in various active ingredients, such as carotenoids, polyphenolic compounds, and polysaccharides. These active ingredients possess powerful antioxidant capabilities, scavenging free radicals induced by ultraviolet radiation and reducing oxidative damage; they can absorb ultraviolet rays, reducing their harm to skin cells; and they can enhance the skin's moisturizing ability and barrier function, promoting skin repair.

[0008] Cornus officinalis extract is rich in various active ingredients, such as ursolic acid and flavonoids. These active ingredients have significant antioxidant functions, capable of scavenging excess reactive oxygen species (ROS) induced by ultraviolet radiation, reducing oxidative stress, and inhibiting the production of ultraviolet-induced inflammatory factors, thus alleviating inflammatory responses.

[0009] Sea buckthorn extract is rich in various active ingredients, such as vitamin E and flavonoids. These active ingredients have strong antioxidant capabilities, effectively scavenging free radicals induced by ultraviolet radiation and reducing oxidative damage; they can also absorb ultraviolet radiation, protecting cell membranes from oxidative damage caused by ultraviolet radiation.

[0010] Shea butter is rich in various active ingredients, such as vitamin E, cinnamic acid esters, and unsaturated fatty acids. These active ingredients can prevent allergic reactions caused by sunlight and have a certain repairing effect on sunburn and sunspots; they also help maintain the integrity of skin cell membranes and enhance the skin's barrier function.

[0011] Camellia seed oil is rich in various active ingredients, such as vitamin E, polyphenols, and unsaturated fatty acids. Ultraviolet radiation triggers the production of numerous free radicals in the skin; the active ingredients in camellia seed oil can scavenge these free radicals, reduce oxidative stress, and protect skin cells. Ultraviolet radiation can also cause skin inflammation; the active ingredients in camellia seed oil can inhibit the production of inflammatory factors and alleviate inflammation.

[0012] Furthermore, the preparation method of the cowhide tea extract includes the following steps:

[0013] S1. Crush the dried cowhide tea leaves and pass them through a 50-80 mesh sieve to obtain cowhide tea leaf powder;

[0014] S2. The cowhide and tea powder obtained in S1 is subjected to ultrasonic-assisted extraction using 10-15 times the mass of an ethanol solution with a concentration of 60-80% v / v, followed by filtration to obtain the extract.

[0015] S3. Purify the extract obtained in S2 using macroporous resin and collect the eluent;

[0016] S4. The eluent obtained in S3 is concentrated under reduced pressure and spray-dried to obtain the cowhide tea extract.

[0017] Furthermore, in step S2, the ultrasonic power is 200-300W, the ultrasonic frequency is 40-60kHz, and the extraction time is 1-2h.

[0018] Furthermore, in step S3, the macroporous resin is one of HPD300, HZ806, or AB-8.

[0019] Furthermore, step S3 specifically includes:

[0020] The pH of the extract was adjusted to 2-2.5 using hydrochloric acid solution to prepare the loading solution;

[0021] After loading the sample solution onto a macroporous resin packed column, elute sequentially with water, 30-40% v / v ethanol solution, and 70-80% v / v ethanol solution at a rate of 2-3 BV / h. Collect the 70-80% v / v ethanol solution eluent.

[0022] Furthermore, the volume ratio of the loading solution to the macroporous resin is 1:2-3; the amount of water used is 8-10 BV; the amount of 30-40% v / v ethanol solution used is 4-6 BV; and the amount of 70-80% v / v ethanol solution used is 2-3 BV.

[0023] A sunscreen product containing 1-10 wt% of the above-mentioned composition for resisting ultraviolet damage.

[0024] Furthermore, the sunscreen product also includes ethylhexyl triazine, phenylbenzimidazole sulfonic acid, and titanium dioxide.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] The composition for combating UV damage in this invention comprises extracts of cowhide and tea leaves, green algae extract, cornus officinalis extract, sea buckthorn extract, shea butter, and camellia seed oil. The composition is rich in various active ingredients, which work synergistically to neutralize free radicals induced by UV radiation, reduce oxidative stress, and protect skin cells from damage; absorb UV radiation, reducing its penetration into the skin and thus minimizing direct UV damage; and regulate tyrosinase activity or block melanin synthesis pathways, reducing melanin accumulation and helping to prevent and improve pigmentation and dullness, thereby achieving the effect of combating UV damage. Detailed Implementation

[0027] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0028] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0029] Avocado (BUTYROSPERMUM PARKI I) fruit oil: purchased from Guangzhou Rongdaxing Chemical Co., Ltd.;

[0030] Camellia oleifera seed oil: purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd.

[0031] Chlorophyta Spp. extract: purchased from Guangzhou GIGABYTE Trading Co., Ltd.;

[0032] Cornus officinalis extract: purchased from Xi'an Zhongkeda Biotechnology Co., Ltd.;

[0033] Sea buckthorn (HIPPOPHAE RHAMNOIDES) extract: purchased from Xi'an Zhongkeda Biotechnology Co., Ltd.

[0034] Example 1: Preparation method of cowhide and tea extract

[0035] Experimental Group 1:

[0036] S1. Crush the dried cowhide tea leaves and pass them through a 50-mesh sieve to obtain cowhide tea leaf powder;

[0037] S2. The cowhide tea powder obtained in S1 was subjected to ultrasonic-assisted extraction using 10 times the mass of an ethanol solution with a concentration of 60% v / v. The solution was then filtered to obtain an extract. The ultrasonic power was 200W, the ultrasonic frequency was 40kHz, and the extraction time was 1h.

[0038] S3. Adjust the pH of the extract to 2 using hydrochloric acid solution to prepare the loading solution. Load the loading solution onto an AB-8 macroporous resin packed column and elute sequentially with 8 BV of water, 4 BV of 30% v / v ethanol solution and 2 BV of 70% ethanol solution at a rate of 2 BV / h. Collect the 70% v / v ethanol solution eluent.

[0039] S4. The eluent obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50°C, and then spray-dried at an inlet air temperature of 170°C and an outlet air temperature of 100°C to obtain the cowhide tea extract.

[0040] Experimental group 2:

[0041] S1. Crush the dried cowhide tea leaves and pass them through an 80-mesh sieve to obtain cowhide tea leaf powder;

[0042] S2. The cowhide tea powder obtained in S1 was subjected to ultrasonic-assisted extraction using 15 times the mass of an 80% v / v ethanol solution. The mixture was then filtered to obtain an extract. The ultrasonic power was 300W, the ultrasonic frequency was 60kHz, and the extraction time was 2h.

[0043] S3. Adjust the pH of the extract to 2.5 using hydrochloric acid solution to prepare the loading solution. Load the loading solution onto an AB-8 macroporous resin packed column and elute sequentially with 10 BV of water, 6 BV of 40% v / v ethanol solution and 3 BV of 80% ethanol solution at a rate of 3 BV / h. Collect the 80% v / v ethanol solution eluent.

[0044] S4. The eluent obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50°C, and then spray-dried at an inlet air temperature of 170°C and an outlet air temperature of 100°C to obtain the cowhide tea extract.

[0045] Experimental group 3:

[0046] S1. Crush the dried cowhide tea leaves and pass them through a 60-mesh sieve to obtain cowhide tea leaf powder;

[0047] S2. The cowhide tea powder obtained in S1 was subjected to ultrasonic-assisted extraction using 13 times the mass of an ethanol solution with a concentration of 70% v / v. The extract was then filtered to obtain an extract with an ultrasonic power of 250W, an ultrasonic frequency of 50kHz, and an extraction time of 1.5h.

[0048] S3. The pH of the extract was adjusted to 2.3 using hydrochloric acid solution to prepare the loading solution. The loading solution was loaded onto an AB-8 macroporous resin packed column and eluted sequentially with 9 BV of water, 5 BV of 35% v / v ethanol solution and 2 BV of 75% ethanol solution at a elution rate of 3 BV / h. The 75% v / v ethanol solution eluent was collected.

[0049] S4. The eluent obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50°C, and then spray-dried at an inlet air temperature of 170°C and an outlet air temperature of 100°C to obtain the cowhide tea extract.

[0050] Experimental group 4:

[0051] S1. Crush the dried cowhide tea leaves and pass them through a 60-mesh sieve to obtain cowhide tea leaf powder;

[0052] S2. The cowhide tea powder obtained in S1 was extracted with 13 times the mass of 70% v / v ethanol solution, filtered, and the extract was obtained. The extraction time was 5 hours.

[0053] S3. The pH of the extract was adjusted to 2.3 using hydrochloric acid solution to prepare the loading solution. The loading solution was loaded onto an AB-8 macroporous resin packed column and eluted sequentially with 9 BV of water, 5 BV of 35% v / v ethanol solution and 2 BV of 75% ethanol solution at a elution rate of 3 BV / h. The 75% v / v ethanol solution eluent was collected.

[0054] S4. The eluent obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50°C, and then spray-dried at an inlet air temperature of 170°C and an outlet air temperature of 100°C to obtain the cowhide tea extract.

[0055] Experimental group 5:

[0056] S1. Crush the dried cowhide tea leaves and pass them through a 60-mesh sieve to obtain cowhide tea leaf powder;

[0057] S2. The cowhide tea powder obtained in S1 was subjected to ultrasonic-assisted extraction using 13 times the mass of an ethanol solution with a concentration of 70% v / v. The solution was filtered to obtain an extract, wherein the ultrasonic power was 250W, the ultrasonic frequency was 50kHz, and the extraction time was 3h.

[0058] S3. The pH of the extract was adjusted to 2.3 using hydrochloric acid solution to prepare the loading solution. The loading solution was loaded onto an AB-8 macroporous resin packed column and eluted sequentially with 9 BV of water, 5 BV of 35% v / v ethanol solution and 2 BV of 75% ethanol solution at a elution rate of 3 BV / h. The 75% v / v ethanol solution eluent was collected.

[0059] S4. The eluent obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50°C, and then spray-dried at an inlet air temperature of 170°C and an outlet air temperature of 100°C to obtain the cowhide tea extract.

[0060] Experimental group 6:

[0061] S1. Crush the dried cowhide tea leaves and pass them through a 60-mesh sieve to obtain cowhide tea leaf powder;

[0062] S2. The cowhide tea powder obtained in S1 was subjected to ultrasonic-assisted extraction using 13 times the mass of an ethanol solution with a concentration of 70% v / v. The extract was then filtered to obtain an extract with an ultrasonic power of 350W, an ultrasonic frequency of 80kHz, and an extraction time of 1.5h.

[0063] S3. The pH of the extract was adjusted to 2.3 using hydrochloric acid solution to prepare the loading solution. The loading solution was loaded onto an AB-8 macroporous resin packed column and eluted sequentially with 9 BV of water, 5 BV of 35% v / v ethanol solution and 2 BV of 75% ethanol solution at a elution rate of 3 BV / h. The 75% v / v ethanol solution eluent was collected.

[0064] S4. The eluent obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50°C, and then spray-dried at an inlet air temperature of 170°C and an outlet air temperature of 100°C to obtain the cowhide tea extract.

[0065] Experimental group 7:

[0066] S1. Crush the dried cowhide tea leaves and pass them through a 60-mesh sieve to obtain cowhide tea leaf powder;

[0067] S2. The cowhide tea powder obtained in S1 was subjected to ultrasonic-assisted extraction using 13 times the mass of an ethanol solution with a concentration of 70% v / v. The extract was then filtered to obtain an extract with an ultrasonic power of 250W, an ultrasonic frequency of 50kHz, and an extraction time of 1.5h.

[0068] S3. Adjust the pH of the extract to 2.3 using hydrochloric acid solution to prepare the loading solution. Load the loading solution onto an AB-8 macroporous resin packed column and elute sequentially with 9 BV of 20% v / v ethanol solution, 5 BV of 35% v / v ethanol solution, and 2 BV of 75% ethanol solution at a rate of 3 BV / h. Collect the 75% v / v ethanol solution eluent.

[0069] S4. The eluent obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50°C, and then spray-dried at an inlet air temperature of 170°C and an outlet air temperature of 100°C to obtain the cowhide tea extract.

[0070] Experimental group 8:

[0071] S1. Crush the dried cowhide tea leaves and pass them through a 60-mesh sieve to obtain cowhide tea leaf powder;

[0072] S2. The cowhide tea powder obtained in S1 was subjected to ultrasonic-assisted extraction using 13 times the mass of an ethanol solution with a concentration of 70% v / v. The extract was then filtered to obtain an extract with an ultrasonic power of 250W, an ultrasonic frequency of 50kHz, and an extraction time of 1.5h.

[0073] S3. The pH of the extract was adjusted to 4 using hydrochloric acid solution to prepare the loading solution. The loading solution was loaded onto an AB-8 macroporous resin packed column and eluted sequentially with 9 BV of water, 5 BV of 35% v / v ethanol solution and 2 BV of 75% ethanol solution at a elution rate of 3 BV / h. The 75% v / v ethanol solution eluent was collected.

[0074] S4. The eluent obtained in S3 is concentrated under reduced pressure at -0.08 MPa and 50°C, and then spray-dried at an inlet air temperature of 170°C and an outlet air temperature of 100°C to obtain the cowhide tea extract.

[0075] Experimental Example 1

[0076] The bovine hide and tea extracts obtained in experimental groups 1-8 were placed at a high temperature of 45℃ for 72 hours. The bovine hide and tea extracts from 0 days and 72 hours at high temperature were then subjected to ABTS free radical scavenging tests and UV radiation resistance measurements.

[0077] 1. ABTS free radical scavenging test method:

[0078] (1) Prepare a 7 mmol / L ABTS stock solution by adding deionized water to ABTS.

[0079] (2) Add potassium persulfate to deionized water to prepare a potassium persulfate stock solution of 140 mmol / L.

[0080] (3) Mix ABTS stock solution and potassium persulfate stock solution in a 1:1 ratio, react in the dark for 12 hours, and then dilute with anhydrous ethanol until the absorbance of the solution is 0.7±0.1 to obtain the working solution.

[0081] (4) The cowhide tea extract was diluted 10 times with 95% v / v ethanol to prepare a sample solution.

[0082] (5) Prepare the test sample by mixing the sample solution and the working solution at a mass ratio of 1:4, let it stand for 6 min, and measure the absorbance at 734 nm. Perform three parallel tests on the sample.

[0083] ABTS free radical scavenging rate = [A - (BC)] / A × 100%

[0084] In the formula, A is the blank control (using deionized water instead of the sample solution); B is the test sample; and C is the sample solution.

[0085] 2. Methods for determining resistance to ultraviolet radiation:

[0086] (1) Take the cowhide and tea extracts obtained from experimental groups 1-8, and dilute each group of cowhide and tea extracts by 50 times with ethanol solution with a volume concentration of 50% to obtain sample solutions.

[0087] (2) An ethanol solution with a volume concentration of 50% was used as a control solution.

[0088] (3) The sample solution and the control solution were scanned in the wavelength range of 280-400 nm using a UV-Vis spectrophotometer. Three parallel experiments were conducted for each experimental group, and the average absorbance values ​​in UVB (280-320 nm) and UVA (320-400 nm) were calculated.

[0089]

[0090] The ABTS free radical scavenging test results showed that the bovine hide tea extract prepared by the method of this invention had a good ABTS free radical scavenging effect. Combined with the analysis of anti-ultraviolet radiation ability, the bovine hide tea extract had a good absorption effect on ultraviolet rays in the 280-400nm wavelength range, indicating that the bovine hide tea extract had strong antioxidant capacity and could reduce the damage of ultraviolet radiation to the skin. After high temperature testing at 45℃, the antioxidant capacity of the bovine hide tea extract prepared in experimental groups 1-3 changed little. In experimental groups 4-8, the adjustment of ultrasonic parameters and resin purification extraction process led to a decrease in the ABTS free radical scavenging effect. Among them, experimental group 4 did not use ultrasonic treatment, which led to a decrease in the ABTS free radical scavenging effect and anti-ultraviolet radiation ability of the bovine hide tea extract. However, the reasonable ultrasonic parameters used in this invention to treat bovine hide tea can promote the release and dissolution of active ingredients in bovine hide tea. In experimental group 5, extending the extraction time and in experimental group 6, increasing the ultrasonic frequency, the bovine hide tea extract obtained The ABTS free radical scavenging effect and UV radiation resistance of the extract were comparable to those of experimental groups 1-3. However, compared to experimental groups 1-3, the ultrasonic parameters used in experimental groups 5-6 resulted in increased energy consumption. In experimental group 7, changing the type of eluent not only reduced the ABTS free radical scavenging effect of the prepared bovine hide and tea extract, but also significantly reduced the ABTS free radical scavenging effect after being placed at a high temperature of 45°C for 72 hours. In experimental group 8, changing the pH value of the loading solution led to a decrease in purification effect, while adjusting the pH value of the loading solution to 2-2.5 in this invention can improve the adsorption of active ingredients by the resin. Therefore, the bovine hide and tea extract obtained in experimental group 3 was selected as the raw material for the composition.

[0091] Example 2: Composition for protection against UV damage

[0092] Processing Group 1:

[0093] The composition for resisting UV damage, by weight, comprises the following ingredients: 5 parts of cowhide tea extract (experimental group 3), 3 parts of green algae extract, 1 part of cornus extract, 8 parts of sea buckthorn extract, 2.5 parts of shea butter and 5 parts of camellia seed oil.

[0094] Processing Group 2:

[0095] The composition for resisting UV damage, by weight, comprises the following ingredients: 8 parts of cowhide tea extract (experimental group 3), 7 parts of green algae extract, 4 parts of cornus extract, 10 parts of sea buckthorn extract, 6 parts of shea butter, and 8 parts of camellia seed oil.

[0096] Processing Group 3:

[0097] The composition for resisting UV damage comprises, by weight, the following ingredients: 6.5 parts of cowhide tea extract (experimental group 3), 5 parts of green algae extract, 2.5 parts of cornus extract, 9 parts of sea buckthorn extract, 4 parts of shea butter and 7 parts of camellia seed oil.

[0098] Processing Group 4:

[0099] The only difference from treatment group 3 is that it does not contain cowhide tea extract, and the missing amount is made up by green algae extract, cornus extract, and sea buckthorn extract in a mass ratio of 5:2.5:9.

[0100] Processing Group 5:

[0101] The only difference from treatment group 3 is that it does not contain green algae extract, and the missing amount is made up by extracts of cowhide tea leaves, cornus officinalis extract, and sea buckthorn extract in a mass ratio of 6.5:2.5:9.

[0102] Processing Group 6:

[0103] The only difference from treatment group 3 is that it does not contain Cornus officinalis extract, and the missing amount is made up by extracts of oxhide tea leaves, green algae extract, and sea buckthorn extract in a mass ratio of 6.5:5:9.

[0104] Processing Group 7:

[0105] The only difference from treatment group 3 is that it does not contain sea buckthorn extract, and the missing amount is made up by extracts of cowhide tea leaves, green algae extract, and cornus officinalis extract in a mass ratio of 6.5:5:2.5.

[0106] Experimental Example 2

[0107] Procedure for tyrosinase inhibition test:

[0108] (1) Tyrosinase and L-tyrosine solution were prepared with PBS buffer to prepare tyrosinase solution of 500 U / mL and L-tyrosine solution of 0.5 g / L, respectively.

[0109] (2) The compositions of treatment groups 1-7 were prepared into 1 wt% test samples using PBS buffer.

[0110] (3) Each treatment group was tested using 12 wells. 50 μL of tyrosine solution was added to each well, and the wells were then divided into four groups (A, B, C, and D), with three wells in each group. Group A contained 100 μL of deionized water and 20 μL of L-tyrosine solution; Group B contained 120 μL of deionized water; Group C contained 100 μL of the test sample and 20 μL of L-tyrosine solution; and Group D contained 120 μL of the test sample. The reaction was carried out at 25 °C for 10 min, and the absorbance was measured at 475 nm to calculate the tyrosinase inhibition rate.

[0111] Tyrosinase inhibition rate = (AB) - (CD) / AB × 100%

[0112] Melanin Inhibition Test:

[0113] The test was conducted in accordance with T / SHRH 036-2021 "Melanin Inhibition Test in Cosmetics - Zebrafish Embryo Test Method".

[0114] The embryo culture medium was prepared by weighing 17.5g of NaCl, 0.76g of KCl, 2.91g of CaCl2, and 2.38g of MgSO4, adding 300mL of deionized water and mixing, then adding 0.714g of HEPES, and adjusting the pH to 7.2 with 1mol / L NaOH to obtain the embryo culture medium.

[0115] The compositions of treatment groups 1-7 were prepared into 1 wt% test samples using water. Each group consisted of 30 zebrafish embryos, and each experiment was performed in triplicate. The experimental group consisted of zebrafish embryos placed in the test samples; the positive control group consisted of zebrafish embryos placed in 0.03 mg / mL phenylthionamide solution; and the blank group consisted of zebrafish embryos placed in embryo culture medium. After exposure in a 28℃ incubator for 48 hours, the melanin signal intensity of the fish embryos was detected and statistically analyzed.

[0116] Melanin inhibition rate (%) = (Average signal intensity of blank group fish embryos - Average signal intensity of experimental group fish embryos) / (Average signal intensity of blank group fish embryos - Average signal intensity of positive control group fish embryos) × 100%

[0117] name Tyrosinase inhibition rate (%) Melanin inhibition rate (%) Processing Group 1 83.4 26.2 Processing Group 2 84.1 25.7 Processing Group 3 84.5 27.1 Processing Group 4 73.4 22.4 Processing Group 5 76.8 21.9 Processing Group 6 74.6 23.8 Processing Group 7 78.2 20.5

[0118] The experimental results show that the composition of the present invention can effectively inhibit tyrosinase activity, inhibit melanin production, and prevent skin from darkening after exposure to ultraviolet radiation.

[0119] Example 3: Sunscreen Products

[0120] Sunscreen products were prepared using the composition of treatment group 3, referring to the ingredient list in the table below:

[0121]

[0122] Comparative Example 1

[0123] Based on Example 3, the composition of treatment group 3 was not added, and the missing amount was made up by an equal amount of deionized water, and the rest was the same as in Example 3.

[0124] Preparation process flow of the sunscreen products in Example 3 and Comparative Example 1:

[0125] Oil phase preparation: Add the oil phase components to the oil phase pot, heat to 70°C, stir evenly to fully dissolve and disperse the components, and form a uniform oil phase.

[0126] Preparation of aqueous phase: Add the aqueous phase components, the composition of treatment group 3 and deionized water to the aqueous phase pot, heat to 75-80℃, stir evenly to fully dissolve each component and form a homogeneous aqueous phase.

[0127] Emulsification: The aqueous phase is slowly added to the oil phase while stirring continuously, controlling the stirring speed to ensure thorough mixing of the oil and water phases and form a primary emulsion. Homogenize at 4000 rpm for 4 minutes to obtain the sunscreen.

[0128] The finished product was tested for SPF and PFA values ​​according to the test methods for sunscreen cosmetics in the "Cosmetic Safety Technical Specifications".

[0129] name PFA SPF Example 3 11 58.9 Comparative Example 1 7 42.7

[0130] Experimental results show that the sunscreen prepared from the composition of the present invention has a very good protective effect.

[0131] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for resisting ultraviolet damage, characterized in that, The composition comprises, by weight, the following raw materials: 5-8 parts of cowhide tea extract, 3-7 parts of green algae extract, 1-4 parts of cornus extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of shea butter and 5-8 parts of camellia seed oil; The preparation method of the cowhide tea extract includes the following steps: S1. Crush the dried cowhide tea leaves and pass them through a 50-80 mesh sieve to obtain cowhide tea leaf powder; S2. The cowhide and tea powder obtained in S1 is subjected to ultrasonic-assisted extraction using 10-15 times the mass of an ethanol solution with a concentration of 60-80% v / v, followed by filtration to obtain the extract. S3. Adjust the pH of the extract to 2-2.5 using hydrochloric acid solution to prepare the loading solution; after loading the loading solution onto a macroporous resin packed column, elute sequentially with water, 30-40% v / v ethanol solution and 70-80% v / v ethanol solution at a rate of 2-3 BV / h, and collect the 70-80% v / v ethanol solution eluent; S4. The eluent obtained in S3 is concentrated under reduced pressure and spray-dried to obtain the cowhide tea extract; In step S2, the ultrasonic power is 200-300W, the ultrasonic frequency is 40-60kHz, and the extraction time is 1-2h.

2. The composition for resisting UV damage according to claim 1, characterized in that, The macroporous resin used in step S3 is one of HPD300, HZ806, and AB-8.

3. The composition for resisting UV damage according to claim 1, characterized in that, The volume ratio of the loading solution to the macroporous resin is 1:2-3; the amount of water used is 8-10 BV; the amount of 30-40% v / v ethanol solution used is 4-6 BV; and the amount of 70-80% v / v ethanol solution used is 2-3 BV.

4. The use of the composition against UV damage according to any one of claims 1-3 in the preparation of sunscreen products.

5. A sunscreen product, characterized in that, A composition containing 1-10 wt% of any one of claims 1-3 for protection against UV damage.

6. The sunscreen product according to claim 5, characterized in that, It also includes ethylhexyl triazine, phenylbenzimidazole sulfonic acid, and titanium dioxide.

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